J. Lippincott-Schwartz

Programmed withdrawal of cilia maintenance followed by centriole capping leads to permanent cilia loss during cerebellar granule cell neurogenesis

The paper identifies a programmed cilia deconstruction pathway in cerebellar granule cells during neurogenesis, showing decreased cilia maintenance and centriole capping prevent cilia regrowth and dysregulated signaling.

Permanent deconstruction of intracellular primary cilia in differentiating granule cell neurons

The paper investigates ciliary dynamics in differentiating granule cell neurons, finding that cilia are deconstructed intracellularly and do not reform in adult neurons.

Nanometer-scale views of visual cortex reveal anatomical features of primary cilia poised to detect synaptic spillover

This study examines primary cilia in mouse visual cortex using electron microscopy, revealing their distribution, structure, and proximity to synapses.

PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity

This study identifies FKBP8 as the tethering partner of PDZD8 at ER-mitochondria contact sites, showing their interdependence in regulating mitochondrial morphology.

Lysosomal release of amino acids at ER three-way junctions regulates transmembrane and secretory protein mRNA translation

The paper shows that lysosomal amino acid release at ER junctions regulates translation of secretome mRNAs, with Lunapark and lysosomal function playing key roles.

Host ZCCHC3 blocks HIV-1 infection and production by a dual mechanism

This study identifies ZCCHC3 as a novel anti-HIV-1 factor that acts through a dual mechanism to block viral infection and production.

Simultaneous photoactivation and high-speed structural tracking reveal diffusion-dominated motion in the endoplasmic reticulum

This study uses photoactivation and high-speed imaging with machine learning to show that proteins in the ER exhibit Brownian diffusion, with structural asymmetry influencing their movement.

Cristae formation is a mechanical buckling event controlled by the inner membrane lipidome

The paper shows that cardiolipin buffers mitochondrial inner membrane curvature and ATP synthase organization, with a mathematical model predicting snapthrough instability in cristae formation.

YAP condensates are highly organized hubs for YAP/TEAD transcription

This study shows YAP forms organized condensates under Hippo pathway and actin tension, which stabilize YAP and recruit coactivators to enhance transcription.

Motion of single molecular tethers reveals dynamic subdomains at ER-mitochondria contact sites

This study uses 3D electron microscopy and live cell imaging to reveal dynamic subdomains at ER-mitochondria contact sites and how VAPB tethers contribute to their structure and remodeling.

An open-access volume electron microscopy atlas of whole cells and tissues

The paper presents a 3D electron microscopy atlas of whole cells and tissues with high resolution and large volume, enabling new biological insights.

Vacuole phase-partitioning boosts mitochondria activity and cell lifespan through an inter-organelle lipid pipeline

The paper identifies a lipid trafficking pathway between yeast vacuoles and mitochondria that enhances mitochondrial activity and cell lifespan under glucose starvation.

Automatic whole cell organelle segmentation in volumetric electron microscopy

The paper develops an automatic pipeline for segmenting and analyzing organelles in whole cells using FIB-SEM data, enabling quantification of spatial interactions and cross-modality registration.

Deuteration improves small-molecule fluorophores

This study shows that deuteration of small-molecule fluorophores enhances their brightness, chromostability, and photostability in cellular imaging.

Image-based pooled whole genome CRISPR screening for Parkin and TFEB subcellular localization

The paper introduces AI-Photoswitchable Screening (AI-PS) to identify genes affecting subcellular localization, validating factors involved in TFEB relocation.

Label-free single-instance protein detection in vitrified cells

This paper introduces a method to detect protein conformations in vitrified cells using high-resolution template matching, revealing insights into ribosome dynamics and ligand binding.

Optimization and functionalization of red-shifted rhodamine dyes

This study optimizes red-shifted rhodamine dyes to improve their performance and enables functionalization with different chemical groups.

Transcriptional reprogramming in fused cells is triggered by plasma-membrane diminution

The paper shows that cell fusion triggers differentiation through plasma-membrane diminution, leading to transient cell starvation and activation of AMPK, which inhibits YAP1 and causes cell-cycle arrest.

Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells

This study develops a platform for 3D correlative cryogenic super-resolution and block-face EM of whole vitreously frozen cells, revealing new protein-ultrastructure relationships.

Phase separation of YAP reorganizes genome topology for long-term YAP target gene expression

This study shows YAP forms liquid-like condensates that reorganize genome topology to enable long-term target gene expression.

ER membranes exhibit phase behavior at sites of organelle contact

The paper demonstrates that ER membranes can phase separate into liquid-ordered and liquid-disordered domains using Large Intra-Cellular Vesicles generated by hypotonic treatment.

Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III

This study identifies Spastin's role in tethering lipid droplets to peroxisomes and facilitating fatty acid trafficking via ESCRT-III proteins.

Cytoplasmic self-organization established by internal lipid membranes in the interplay with either actin or microtubules

The paper demonstrates that lipid membranes can organize both microtubules and actin, even when one cytoskeletal system is paralyzed, revealing a universal principle of cytoplasmic self-organization.

mTOR-dependent phosphorylation controls TFEB nuclear export

This study identifies mTOR-dependent phosphorylation of TFEB as a mechanism controlling its nuclear export and activity during nutrient re-feeding.

Immature HIV-1 Lattice Assembly Dynamics are Regulated by Scaffolding from Nucleic Acid and the Plasma Membrane

This study investigates how HIV-1 Gag polyprotein assembly is regulated by RNA and the plasma membrane using computational and experimental methods, revealing scaffolding roles for these components.