X. Darzacq

TFEB controls syncytiotrophoblast differentiation

This study identifies TFEB as a transcriptional regulator of syncytiotrophoblast differentiation by controlling Syncytin-1 and Syncytin-2 expression in BeWo cells.

Oblique Line Scan Illumination Enables Expansive, Accurate and Sensitive Single Protein Measurements in Solution and in Living Cells

This paper introduces Oblique Line Scan (OLS) illumination to enhance single-molecule tracking in living cells and solution, enabling high-throughput and sensitive protein dynamics measurements.

Surprising Features of Nuclear Receptor Interaction Networks Revealed by Live Cell Single Molecule Imaging

The paper uses live cell imaging to show that RAR, not RXR, limits heterodimerization and chromatin binding of T2NRs, challenging previous assumptions about RXR's role.

p300 Is an Obligate Integrator of Combinatorial Transcription Factor Inputs

This study shows p300 requires multiple transcription factor interaction domains for chromatin binding and catalytic regulation, highlighting the necessity of combinatorial TF inputs for coactivator function.

A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms

This paper introduces a high-throughput single-molecule tracking platform to study protein dynamics and drug interactions, identifying novel mechanisms and therapeutics.

Proximity-assisted photoactivation (PAPA): Detecting molecular interactions in live-cell single-molecule imaging

This paper introduces PAPA, a method using rhodamine dyes to detect molecular interactions in live-cell single-molecule imaging by reactivating a fluorophore from a dark state when proximal to another.

Recovering mixtures of fast diffusing states from short single particle trajectories

This paper develops methods to infer diffusion coefficient distributions from short single particle trajectories in SPT data, addressing technical limitations in tracking fast-diffusing proteins.

Structure of the human SAGA coactivator complex

The paper presents the 2.9 Å structure of the human SAGA coactivator complex, revealing unique structural features and functional modules with implications for transcription and splicing in disease and cancer.

Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments

The paper uses single-molecule techniques to show catalysis does not enhance diffusion of alkaline phosphatase, contradicting previous FCS results attributed to substrate-induced dye blinking.

A new class of disordered elements controls DNA replication through initiator self-assembly

This study identifies a new class of disordered elements that drive liquid-liquid phase separation in DNA replication initiators in D. melanogaster.

Determining cellular CTCF and cohesin abundances to constrain 3D genome models

The paper quantifies CTCF and cohesin levels in mammalian cells to improve 3D genome models and provides a method for measuring Halo-tagged proteins.

An RNA-binding region regulates CTCF clustering and chromatin looping

The paper shows that an RNA-binding region in CTCF mediates chromatin looping and clustering, revealing two classes of CTCF loops: RBR-dependent and RBR-independent.

Guided nuclear exploration increases CTCF target search efficiency

This study shows that CTCF uses RNA-binding region-mediated trapping to increase its target search efficiency in the nucleus by ~2.5 fold.

Spot-On: robust model-based analysis of single-particle tracking experiments

Spot-On provides a bias-corrected analysis tool for single-particle tracking experiments, outperforming existing methods and demonstrating robust inference of diffusion constants and subpopulations.

A Versatile Compressed Sensing Scheme For Faster And Less Phototoxic 3D Fluorescence Microscopy

This paper introduces a 3D compressed sensing method for fluorescence microscopy that reduces light exposure and acquisition time by 5-10 fold without hardware changes.

CTCF and Cohesin Regulate Chromatin Loop Stability with Distinct Dynamics

This study shows CTCF and cohesin form a dynamic complex with distinct binding dynamics, suggesting chromatin loops constantly break and reform.