Jonathan M. Houseley

The paper shows that multiple mechanisms suppress meiotic recombination near chromosome ends in yeast, involving both axis protein downregulation and DNA break suppression.

This paper shows that activating AMPK without inhibiting fatty acid synthesis preserves late-life fitness in yeast by modulating acetyl-CoA metabolism.

This study shows that osimertinib treatment leads to a Drug Tolerant Persister state with replication defects, genome instability, and hypersensitivity to DNA repair inhibitors.

This study identifies on-target WRN mutations as resistance mechanisms in MSI cancer cells to WRN helicase inhibitors and suggests combination therapies to prevent resistance.

This study identifies ZFP36L1 and ZFP36L2 as RNA-binding proteins that regulate the G2-M checkpoint in GC B cells to prevent replication stress and maintain genome stability.

TrAEL-seq is a method for profiling DNA replication dynamics in mammalian cells without requiring synchronization or drugs, showing replication fork speed and initiation zone effects.

This study investigates Flp-nickase-induced homologous recombination, revealing two-ended repair mechanisms and their dependence on BRCA1 and BRCA2/RAD51.

The paper shows that an early-life galactose diet in yeast reduces age-related gene dysregulation and senescence markers, suggesting dietary change can mimic caloric restriction benefits without calorie reduction.

This study shows that the Fork Protection Complex reduces DNA damage in regions prone to topological stress but increases genome instability in long replicons by promoting rapid replication.

The paper shows that DNA replication during MEK inhibition drives BRAF amplification and resistance in COLO205 cells, and that blocking cell cycle entry can suppress resistance.

This study shows that chromosome XII fragments, not ERCs, drive senescence in yeast, despite rDNA copy number increases with age.

The paper investigates how transcriptional activation of the CUP1 gene in budding yeast leads to copy number variation through replication stress and homologous recombination.

This paper introduces TrAEL-seq, a method to detect DNA replication fork stalling and double strand breaks with high resolution, and demonstrates its application in yeast and mammalian cells.

This paper introduces glyoxal fixation as a method to preserve RNA quality in flow-sorted cells stained for antigens, enabling transcriptome analysis of purified cell populations.

This study shows that transcription of the CUP1 gene in aged yeast leads to the formation of extrachromosomal DNA, which accumulates without phenotypic selection and is site-specific.

This study shows that H3K4me3 is essential for maintaining normal gene expression levels across yeast lifespan, with COMPASS mutations reducing lifespan and causing gene expression defects.