Justin Rhoades

Impact of preanalytical factors on liquid biopsy in the canine cancer model

This study evaluates how preanalytical factors affect liquid biopsy in canine cancer models, finding that tumor content and metrics vary by blood draw location and cancer type.

MEDUSA: Maintaining Entire DNA Duplexes for Utmost Sequencing Accuracy

MEDUSA improves sequencing accuracy by minimizing DNA duplex resynthesis during library preparation, achieving lower residual SNV frequency in genomic and cell-free DNA.

Molecular determinants of antibody-mediated priming to enhance detection of ctDNA

This paper identifies molecular features of monoclonal antibodies that enhance ctDNA detection by increasing its concentration in blood.

DirectHRD enables sensitive scar-based classification of homologous recombination deficiency (HRD)

DirectHRD is a whole-genome sequencing-based classifier that improves HRD detection sensitivity in low tumor fraction samples.

FinaleMe: Predicting DNA methylation by the fragmentation patterns of plasma cell-free DNA

FinaleMe predicts DNA methylation and tissues-of-origin from plasma whole-genome sequencing by using a non-homogeneous Hidden Markov Model.

A nanoparticle priming agent reduces cellular uptake of cell-free DNA and enhances the sensitivity of liquid biopsies

A nanoparticle priming agent increases cfDNA levels in blood, improving ctDNA detection sensitivity in tumor-bearing mice.

Duplex-Repair enables highly accurate sequencing, despite DNA damage

The paper identifies DNA strand resynthesis as a source of false mutations in sequencing and introduces Duplex-Repair to reduce this error, improving sequencing accuracy.

MAESTRO affords 'breadth and depth' for mutation testing

MAESTRO enables efficient and accurate tracking of thousands of low-frequency mutations with reduced sequencing, improving mutation testing in cancer and liquid biopsies.