Brigitte N. Gomperts

Modeling cell-cell interactions to advance drug discovery in Idiopathic Pulmonary Fibrosis

This study develops a 3D co-culture organoid model of IPF to investigate epithelial-fibroblast interactions and test anti-fibrotic drugs.

Transcriptional and spatial profiling of fibroblasts from human lungs highlights CTHRC1+ cells as fibrogenic signaling hubs in fibrosis

The paper integrates single-cell RNA sequencing and spatial transcriptomics to identify CTHRC1+ fibroblasts as key fibrogenic cells in IPF, showing how culture affects their transcriptional profile.

Bidirectional fibrogenic cross-talk revealed in a human iPSC-derived epithelial-mesenchymal co-culture model of pulmonary fibrosis

The paper establishes a human iPSC-derived co-culture model of pulmonary fibrosis that reveals bidirectional fibrogenic epithelial-mesenchymal crosstalk and identifies potential druggable pathways.

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site-Specific Integration of CFTR and Mutation-Agnostic Disease Rescue

This paper develops lipid nanoparticles to deliver CRISPR/Cas9 for site-specific CFTR gene integration in CF airway cells, achieving 3-3.5% gene editing and 80% Cl- current restoration.

Investigation of Double-Stranded DNA Donors and CRISPR-Cas9 RNP for Universal Correction of Mutations Causing Cystic Fibrosis in Human Airway Cells

This study demonstrates that low levels of CFTR integration can restore chloride ion currents in human airway cells using optimized nonviral CRISPR-Cas9 RNP and double-stranded DNA donors.

High Throughput Screening with a Primary Human Mucociliary Airway Model Identifies a Small Molecule with Anti-SARS-CoV-2 Activity

This study uses a primary human stem cell-based airway model to screen 2100 compounds and identifies a small molecule effective against SARS-CoV-2 and its Omicron variant.

Loss of cell junctional components and matrix alterations drive cell desquamation and fibrotic changes in Idiopathic Pulmonary Fibrosis

The study identifies loss of cell junctions and matrix dysregulation as drivers of epithelial desquamation and fibrosis in IPF distal bronchioles.

Dedifferentiated early postnatal lung myofibroblasts redifferentiate in adult disease

The paper identifies dedifferentiated early postnatal lung myofibroblasts that persist into adulthood and redifferentiate in response to injury.