Bobae An

Multiplexed, scalable analog recording of gene regulation dynamics over weeks using intracellular protein tapes

This paper introduces CytoTape, a tool for continuous, multiplexed recording of gene regulation dynamics over weeks at single-cell resolution, and demonstrates its use in measuring transcription factor activities.

Human brain organoids record the passage of time over multiple years in culture

This study shows human brain organoids can mature over 5 years in culture, transcriptionally aging and retaining memory of time spent in vitro.

Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context

The paper introduces PRISM, a platform that uses combinatorial protein barcodes to enable self-correcting neuron tracing with molecular context, improving tracing accuracy and handling spatial gaps.

Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues

The paper introduces umExM, a method for dense membrane labeling and expansion microscopy to achieve nanoscale visualization of membranes in tissues, demonstrating resolution down to ~35 nm.

Recording of cellular physiological histories along optically readable self-assembling protein chains

The paper introduces a method to record cellular physiological histories using self-assembling protein chains that can be optically read, demonstrating its use in neurons and mouse brain.