Antonio Virgilio Failla

MINFLUX-nanoscopy of hNAIP/NLRC4 inflammasome activation in single human macrophages

This study uses MINFLUX nanoscopy to visualize hNAIP/NLRC4 inflammasome activation in human macrophages, revealing speck formation and variability in NLRC4 disc numbers and hNAIP content.

STED-FCS in subdiffraction limit volumes reveals altered diffusion in live cell applications

This study demonstrates the application of STED-FCS in live cell assays to analyze molecular diffusion, showing its utility in studying autophagy, glycoprotein dynamics, and lipid mobility in disease contexts.

Amphisome biogenesis couples synaptic autophagy to local protein synthesis

The paper shows that amphisomes formed at presynaptic boutons during sustained synaptic activity facilitate protein degradation and local replenishment through mRNA translation.

Mutations affecting the N-terminal domains of SHANK3 point to different pathomechanisms in neurodevelopmental disorders.

The paper identifies a SHANK3 missense mutation linked to ADHD-like phenotype and shows it disrupts protein interactions critical for neurodevelopmental function.

BRAIN-DERIVED EXTRACELLULAR VESICLES ARE HIGHLY ENRICHED IN THE PRION PROTEIN AND ITS C1 FRAGMENT: RELEVANCE FOR CELLULAR UPTAKE AND IMPLICATIONS IN STROKE

This study characterizes brain-derived extracellular vesicles (sEVs) in mice under normal and post-stroke conditions, showing increased PrP and PrP-C1 content and altered cellular uptake.

SIPA1L2 controls trafficking and signaling of TrkB-containing amphisomes at presynaptic terminals

This study shows SIPA1L2 connects TrkB-containing amphisomes to dynein for retrograde trafficking and local signaling at presynaptic terminals, essential for BDNF-dependent plasticity.