Berta Puig

Amyloid-β 42-carrying extracellular vesicles are associated with neurodegeneration and neuroinflammation in Alzheimer's disease

This study identifies Aβ42-carrying extracellular vesicles in plasma as biomarkers associated with neurodegeneration and neuroinflammation in Alzheimer's disease.

Enrichment of Extracellular Vesicles Subsets from Diverse Biological Sources Using Conventional and Image-Based Fluorescence Activated Sorting

This paper develops and validates an optimized workflow for isolating and characterizing extracellular vesicle subsets from diverse sources using FACS and imaging techniques.

Binding of extracellular vesicles to stretched von Willebrand factor promotes platelet activation

The paper shows that extracellular vesicles bind to stretched von Willebrand factor, promoting platelet activation and aggregation, which can trap circulating tumor cells.

Efficient enzyme-free isolation of brain-derived extracellular vesicles

This paper demonstrates that non-enzymatic methods can isolate brain-derived extracellular vesicles without compromising protein integrity, achieving high yield and purity.

Cleavage site-directed antibodies reveal the prion protein in humans is shed by ADAM10 at Y226 and associates with misfolded protein deposits in neurodegenerative diseases

This study identifies ADAM10-mediated shedding of human PrP at Y226 and shows sPrP associates with misfolded protein aggregates in neurodegenerative diseases.

Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult in vitro and in vivo

This study identifies cystatin C as a neuroprotective protein upregulated after stroke and shows that delivering it via brain-derived extracellular vesicles improves synaptic preservation in vitro and in vivo.

Release of VAMP5-positive extracellular vesicles by retinal Muller glia in vivo

The paper identifies VAMP5 as a SNARE component in extracellular vesicles released by retinal Muller glia and shows differences in their secretomes compared to neurons.

Multiplexed mRNA analysis of brain-derived extracellular vesicles upon experimental stroke in mice reveals increased mRNA content related to inflammation and recovery processes

Ligands binding to the cellular prion protein induce its protective proteolytic release with therapeutic potential in neurodegenerative proteinopathies

The paper shows that ligands binding to PrPC induce its proteolytic release, which has neuroprotective effects, and identifies mechanisms and exceptions in this process.

BRAIN-DERIVED EXTRACELLULAR VESICLES ARE HIGHLY ENRICHED IN THE PRION PROTEIN AND ITS C1 FRAGMENT: RELEVANCE FOR CELLULAR UPTAKE AND IMPLICATIONS IN STROKE

This study characterizes brain-derived extracellular vesicles (sEVs) in mice under normal and post-stroke conditions, showing increased PrP and PrP-C1 content and altered cellular uptake.