genetic perturbation / CRISPR

This study uses chromosome-level genomics and cross-species enzyme screening to enhance bakuchiol production in Yarrowia lipolytica through metabolic engineering.

The paper uses CRISPR-based lineage recording and single-cell RNA sequencing to show that mesodermal cell diversity in mouse embryos arises from spatially distinct, fate-biased progenitor pools rather than simple branching lineages.

This study shows that neuronal Aralar deficiency causes ASD-like social behavior deficits through dopaminergic and redox dysfunction, which are reversed by β-hydroxybutyrate treatment.

The paper investigates how adaptive tracking contributes to the invasion success of Drosophila suzukii by showing rapid evolution of thermal tolerance and other traits in response to environmental conditions.

This study investigates the role of cWnt signaling in planarian regeneration, showing it regulates stem cell proliferation and differentiation of neural, muscle, and parenchymal tissues during whole-body regeneration.

This paper optimizes dCas12a-based CRISPRi/a for pooled screening in Yarrowia lipolytica, identifying effective crRNA features and a high-performing effector variant.

The paper engineers a highly efficient PAMless genome editing system, eTasR, which significantly improves editing efficiency in mammalian cells and enables in vivo gene disruption.

This study shows HPV oncogenes alter DNA repair mechanisms in HNSCC, creating a vulnerability to therapies targeting RAD51 filament dynamics.

This paper develops a method to sustain Tie2 activation using engineered mRNA, showing improved endothelial stabilization compared to traditional ligands.

This study identifies S100A4 as a targetable regulator of immune suppression in glioblastoma, showing its inhibition enhances immunotherapy response and survival.

This paper identifies a SOX9 C-terminal module that recruits cBAF to enhancers, controlling cell state by remodeling chromatin.

This study shows a dynamin mutation causes neuropathy-like phenotypes by affecting microtubule stabilization through Patronin/CAMSAP interaction in Drosophila and mammalian cells.

The paper identifies PKR as a critical mediator of mitochondrial neuropathology in a mouse model of Leigh syndrome, showing its role in neuroinflammation and disease progression.

This study shows increasing PCAF-1 in C. elegans extends lifespan, preserves healthspan, and enhances stress responses by reprogramming transcription and improving proteostasis.

The paper develops a high-fidelity CRISPR-Cas13d system for RNA depletion in Plasmodium falciparum, achieving significant target mRNA reduction and functional impairment of sexual differentiation.

The paper designs and tests composite biosensor-gate circuits in soil bacteria to improve environmental synthetic biology applications.

The paper identifies RabE and RabA-mediated trafficking as distinct pathways in plant pre-invasive immunity, with RabE specific to powdery mildew and RabA to oomycete pathogens.

This study shows that cortical ER-PM contact sites maintain nuclear envelope integrity and proper mitotic nuclear segregation by preventing membrane damage and supporting nuclear dynamics.

The paper shows that glutamate and neuropeptide signaling in C. elegans males convert brief mate contact into a persistent search behavior.

This study shows non-coding RNA disrupts enhancer-promoter interactions when increased, and stabilizes them when decreased, independent of transcription initiation.

This study identifies TRIM11 as a regulator of EWSR1::ATF1 stability in clear cell sarcoma, showing its depletion leads to metabolic rewiring and cell death, with PCK1 as a potential biomarker.

This study uses gene-resolved metagenomics to analyze nitrogen transformation potential in a landfill leachate SBR microbiome, revealing gene distribution across taxa and potential nitrogen retention pathways.

This study shows KAT6 and KAT7 maintain mitotic chromatin acetylation and composition, supporting postmitotic transcriptional memory and developmental competence in stem cells.

This paper identifies a TMEM175 mutation causing short QT syndrome and sudden cardiac death by disrupting lysosomal-mitochondrial interactions and impairing mitochondrial function.

The paper uses in vivo single-cell CRISPR screens to study how tumor suppressor loss and genetic perturbations affect glioma states and immune recruitment in mice.

The paper identifies essential C1-fixing cluster genes in Clostridium autoethanogenum using gene deletions and functional analysis, revealing their roles in autotrophy and metabolism.

The paper identifies SEC16A/MAG5 as essential for ABA receptor homeostasis by mediating ER export and trafficking, impacting ABA signaling without affecting ABA levels.

This study identifies fss genes that regulate stalked cell morphogenesis and cell cycle progression independently of the flagellum in Caulobacter crescentus.

This study identifies SAMP1 as a protector of alveolar type 2 cells against DNA damage-induced senescence and shows its role in limiting pulmonary fibrosis in a mouse model.

This study demonstrates the transferability of homoserine lactone sensors between Bacillales bacteria, enabling programmable interphyla communication between Gram-negative and Gram-positive species.