Bacterial Genetics and Biotechnology

This study shows that deletion of the ravA-viaA operon in Vibrio cholerae increases tolerance to aminoglycosides and affects polymyxin B sensitivity and vancomycin resistance by modulating Cpx and Zra2 two-component systems.

This study shows that ParA's interactions with PopZ and DNA are critical for parS release during chromosome segregation in Caulobacter crescentus, with ParA-R195E causing cell death by preventing proper segregation.

This study uses transcriptomics and proteomics to analyze the thermal stress response in Pyrococcus furiosus, revealing rapid transcriptome reprogramming and persistent protein upregulation during heat shock.

This study investigates how Escherichia coli uses potassium concentration changes for chemotaxis, revealing distinct responses from two chemoreceptors and linking potassium sensing to intracellular pH changes.

The paper shows that bovine annexin A4 expression in E. coli reverses beta-lactam antibiotic-induced cytokinesis block, requiring calcium binding and membrane interaction.

The paper identifies tartrolon B as an inducer of the timABR operon in L. monocytogenes, revealing its role in sensing and detoxifying this antibiotic produced by soil bacteria.

This study identifies RcrB as a key protein conferring resistance to hypochlorous acid in uropathogenic Escherichia coli, showing its protective role against RCS but not ROS.

The paper shows that TerC family proteins are involved in metalation of enzymes during secretion via the Sec pathway, specifically for manganese-dependent enzymes.

This study investigates the role of the NtrB-NtrC two-component system in Caulobacter nitrogen metabolism and development, revealing its impact on growth, cell cycle regulation, and polysaccharide synthesis.

This study demonstrates the use of ligand-displaying E. coli cells and minicells to deliver toxic payloads via T4SSs, enhancing conjugative transfer and killing specific bacterial targets.

The paper identifies and functionally characterizes novel stress-resistance genes from extreme environment metagenomes and demonstrates their ability to enhance bacterial tolerance to multiple stress conditions.

This paper identifies bacterial SEA-like (SEAL) domains that undergo autoproteolysis and function in mechanotransduction, similar to eukaryotic SEA domains.

This paper investigates how the T4aP machine is polarly localized in M. xanthus, showing that PilQ's AMIN domains bind peptidoglycan to enable Tgl recruitment, which drives secretin multimerization.

This study investigates how ParB-DNA interactions form a dynamic partition condensate for bacterial chromosome segregation using in vitro experiments and simulations.

This study identifies the regulons of two alternative sigma factors in Chlamydia trachomatis by using CRISPR interference and RNA sequencing, showing their essential roles in late-stage development.

This paper shows that host use of metallophores to secrete copper intoxicates Staphylococcus aureus during infection.

This study investigates the cell cycle-dependent regulation of the ParABS system in Bdellovibrio bacteriovorus, revealing how ParA:ParB ratios and chromosomal context influence centromere organization during its unique non-binary division.

This study identifies FipA as a novel protein required for FlhF-mediated polar flagellum synthesis in multiple gamma-proteobacteria, independent of HubP.

The paper shows that fever-like temperature increases induce competence in Streptococcus pneumoniae via an HtrA-dependent pathway, independent of other stress signals.

The paper shows that Escherichia coli cells can enter a stress-prepared state that persists for generations but is lost upon dilution, indicating a transient, reversible cellular memory rather than genetic mutation.

This study identifies three genes, strB, rpsL, and rsmG, that control streptomycin susceptibility in Agrobacterium fabrum.

This paper visualizes competence pili in Bacillus subtilis using fluorescence microscopy, showing they are retractile, DNA-binding structures distributed along the cell's long axis.

This paper develops a leakage-free gene expression system in Fusobacterium nucleatum by combining a xylose-inducible promoter with a theophylline-responsive riboswitch, demonstrating precise control of gene expression and identifying essential genes.

This study identifies small regulatory RNAs (sRNAs) as mediators of the SloR and manganese regulons in Streptococcus mutans, linking sRNA expression to virulence gene control and metal ion homeostasis.

This study identifies ArcA as a conserved metabolic regulator in Gram-negative bacteria during bacteremia, showing its role in adapting to oxygen, iron, and membrane stress.

T9GPred predicts Type 9 secretion system, gliding motility, and associated secreted proteins in Bacteroidetes strains using curated experimental data and signal domain analysis.

This study identifies FliL as essential for flagellar motor remodeling during swarming in diverse bacteria, showing conservation across species and a specific interaction with FliF.

The paper genetically manipulates Saccharibacteria from the CPR to study their role in epibiotic growth and develops bioinformatic resources for their analysis.

This study investigates the role of the periplasm and periplasmic proteins in copper homeostasis in Gram-negative bacteria, showing that the periplasm acts as a sensor and storage for copper to adapt to varying environmental concentrations.

This study investigates the roles of DivIVA and GpsB in S. aureus cell cycle and morphogenesis, finding that GpsB regulates cell shape by affecting peptidoglycan synthesis.