Fungal and yeast genetics research

Deep quantitative phosphoproteomics identifies non-canonical pH-sensitive yeast phosphorylation networks

The paper identifies non-canonical pH-sensitive phosphorylation networks in yeast using deep phosphoproteomics, revealing distinct regulatory mechanisms outside of known pathways.

PYEAST - Python Enabled Automated Strain Transformation

PYEAST is a digital toolkit that automates strain transformation in S. cerevisiae using modern DNA synthesis techniques.

Convergent evolution of aerobic fermentation through divergent mechanisms acting on key shared glycolytic genes

The paper identifies convergent evolution of aerobic fermentation in yeasts through divergent genetic mechanisms affecting shared glycolytic genes.

In-situ Target Base Editing Combining with Biosensor-driven Strategy Reveals Critical Single Nucleotide Variants for Enhanced Recombinant Protein Secretion in Pichia pastoris

The paper develops a strategy combining genome editing and biosensor-assisted sorting to identify SNVs enhancing recombinant protein secretion in Pichia pastoris, achieving a high rHSA titer.

Characterizing the endopeptidase activity of Candida albicans Gpi8, a crucial subunit of the GPI transamidase

This paper studies the endopeptidase activity of C. albicans Gpi8 using steady-state kinetics and molecular dynamics simulations to understand substrate binding and cleavage mechanisms.

Heteromorphic tertiary structures of MAT1-1-1 and MAT1-2-1 protein variants simultaneously produced in pairs by Ophiocordyceps sinensis strains

The paper shows that MAT1-1-1 and MAT1-2-1 proteins from O. sinensis strains have divergent tertiary structures, supporting self-sterility and hybrid reproduction.

Establishment of a Congenic Strain for the Oyster Mushroom Reveals the Structure and Evolution of Mating-Type Loci

The paper develops a congenic strain of Pleurotus ostreatus by introgressing mating-type loci from one strain into another, revealing the multiallelic structure of these loci and their genomic organization.

A stage-resolved map of dynamic septin interactions required for infection by the rice blast fungus

The paper maps dynamic septin interactions in the rice blast fungus during infection, revealing roles beyond cytoskeletal organization in plant invasion.

The penta-EF-hand protein Pef1 of Candida albicans functions at sites of membrane perturbation to support polarized growth and membrane integrity

This paper identifies Pef1 as a fungal protein that localizes to sites of membrane disruption in Candida albicans to maintain membrane integrity and support polarized growth.

Calcineurin-responsive zinc finger 1 (Crz1) contributes to stress tolerance and virulence in the pathogenic fungus Trichosporon asahii

This study shows that Crz1 in Trichosporon asahii is involved in stress tolerance and virulence, but its role is smaller than that of calcineurin.

CTG clade-specific proteins of the RSC chromatin remodeling complex regulate cell cycle progression of a critical priority fungal pathogen, Candida albicans

This study identifies Nri1 and Nri2 as CTG clade-specific proteins of the RSC complex in C. albicans that regulate cell cycle progression and fitness.

Regulation of the cell wall integrity pathway at the contact site between mating partners in yeast

The paper shows that the Cell Wall Integrity pathway is downregulated at mating contact sites in yeast to allow cell wall degradation, with Fig2 mediating this process.

Contrasting evolutionary forces of specialization and admixture underlie the genomic and phenotypic diversity of Yarrowia lipolytica

This study examines genomic and phenotypic diversity in Yarrowia lipolytica, showing how specialization and admixture drive adaptation to different environments and industrial traits.

Cis-regulatory elements orchestrate phase-specific effector gene expression in Ustilago maydis

The paper identifies cis-regulatory elements in Ustilago maydis that control effector gene expression during different infection phases.

High levels of Cdc42 GTPase underlie an all-or-none decision to fuse

This study shows that high Cdc42 levels are required for cell fusion in yeast, with distinct regulatory mechanisms compared to polarized growth.

Dissecting the homeodomain MAT locus and engineering novel tripolar and bipolar mating systems in Cryptococcus amylolentus

This study investigates the role of HD genes in sexual reproduction and demonstrates a tetrapolar-to-bipolar transition in Cryptococcus amylolentus using CRISPR-Cas9 gene editing.

Multiple nuclei means multiple chromosome sets in Botrytis cinerea and Neurospora crassa

The paper investigates the nuclear composition of fungal spores in Botrytis cinerea and Neurospora crassa, finding evidence that multiple nuclei are mitotic copies rather than sharing a single haploid set of chromosomes.

Msc1 facilitates glucose starvation-induced remodeling of the nucleus-vacuole junction

This study identifies Msc1 as a glucose starvation-responsive factor that facilitates NVJ remodeling and transcriptional activation in yeast.

Improved adenine-HPLC method for quantifying yeast based on cellular DNA content

The paper improves the adenine-HPLC method to accurately quantify budding yeast by suppressing adenine release from non-DNA sources and correcting for background adenine levels.

Homeodomain protein Sxi1α independently controls cell-cell fusion and gene expression during sexual reproduction in Cryptococcus deneoformans

This study identifies Sxi1's inhibitory role in unisexual cell fusion and distinct transcriptional regulation from Sxi2a in Cryptococcus deneoformans.

Engineering serine metabolism to enhance AOX1 promoter self-induction in a formate dehydrogenase-deficient Komagataella phaffii

This study enhances AOX1 promoter self-induction in K. phaffii by engineering serine metabolism to increase formate production, improving recombinant protein productivity without methanol.

Genetic and environmental determinants of multicellular-like phenotypes in fission yeast

The paper identifies genetic and environmental factors influencing multicellular-like phenotypes in fission yeast through experimental and genomic analyses.

A translational checkpoint couples proline sensing to mitochondrial proline catabolism in Candida glabrata

This paper identifies a regulatory cascade linking proline catabolism to translational repression in Candida glabrata, showing its role in stress adaptation and virulence.

A CROSS-SPECIES ANALYSIS OF CELL WALL MECHANOSENSORS

The paper heterologously expresses fungal Wsc mechanosensors in fission yeast to study cross-species mechanosensing, finding that domain variations affect sensor function.

In planta-specific transcriptional regulatory circuit regulates expression of MoHTR1, a nuclear effector gene of Magnaporthe oryzae

The paper identifies an in planta-specific cis-element, IPA, that regulates the expression of the fungal effector gene MoHTR1 and other related genes, revealing its role in virulence and host immune response.

Homothallic or heterothallic? A genomic investigation into the sexual capabilities of the ascomycete fungus Clonostachys rosea

This paper investigates the sexual reproduction modes of Clonostachys rosea by analyzing MAT1 loci and linkage disequilibrium, finding both homothallic and heterothallic lineages within the species.

FAMeDB: A curated Database for the analysis of Fungal Aromatic Compound Metabolism

FAMeDB is a curated database of fungal proteins involved in aromatic compound metabolism, providing tools for omics analysis and genomic comparisons across 65 fungal species.

Quiescence improves Candida albicans survival of fungicidal drug exposure

This study shows that quiescence in Candida albicans enhances survival during antifungal drug exposure by altering cellular properties and gene expression.

Aneuploidy alleviates the cell proliferation defect caused by mutations affecting origin licensing in Saccharomyces cerevisiae

The paper shows that aneuploidy in yeast can enhance cell proliferation by extending the G1 phase, compensating for defects in DNA replication licensing.

Jen1 transport and endocytosis in yeast reveal pH-responsive strategies to prevent metabolites loss

This study investigates how pH affects Jen1 transporter endocytosis in yeast, revealing pH-dependent strategies to prevent metabolite loss.