Fungal and yeast genetics research

Characterization of velvet DNA-binding region by Aspergillus nidulans VelB

This study identifies three conserved arginine residues in the velvet DNA-binding region of Aspergillus nidulans VelB essential for function, showing their role in electrostatic interactions and DNA recognition.

Studying Effects of PDA Media Strengths on the Growth of Colletotrichum sublineola Using MPLEx-Based Integrative Proteomics and Metabolomics Analyses

This study investigates how varying PDA media strengths affect the growth of Colletotrichum sublineola using proteomics and metabolomics, revealing nutrient limitation-induced sporulation and metabolic reprogramming.

The genetics of transcriptional responses to stress in yeast

This study identifies context-specific eQTLs and their dynamic effects on gene expression in yeast under acute environmental stress, linking genetic variation to physiological adaptation.

Multiple ammonium transporters in fission yeast are coordinated by transcriptional and localization regulation in response to nitrogen starvation

The paper investigates how nitrogen starvation regulates ammonium transporters in fission yeast through transcriptional and localization mechanisms.

Population-scale chemical response revealed by a barcoded yeast collection

The paper develops a barcoded yeast collection to map genotype-phenotype relationships across genetic diversity, revealing genetic variants linked to chemical responses.

Pharmacological stress exposes hidden allelic background effects in genetic interaction screen normalisation

The paper shows that pharmacological stress reveals hidden allelic background effects in genetic interaction screens, using fission yeast and Torin1 to demonstrate divergent fitness profiles.

Surprising regulatory plasticity for the conserved HOG pathway in diverse Saccharomyces cerevisiae strains

The paper shows that Hog1 in wild S. cerevisiae strains has a broader stress response than in lab strains, with distinct non-osmotic stress gene regulation.

Systematic characterization of the yeast secretome under diverse proteosynthetic stress conditions reveals secretion of functional ER chaperone BiP

The paper characterizes the yeast secretome under various proteosynthetic stress conditions and identifies BiP as a functional extracellular chaperone in specific stress scenarios.

Investigating the role of phosphodiesterase Pde2 in coordinating the yeast Environmental Stress Response

This study investigates how Pde2 regulates the yeast Environmental Stress Response by affecting Msn2/4 activation and target gene expression, with minimal impact on Dot6 repression.

Evolution of regulatory networks controlling plasticity in gene expression between Saccharomyces cerevisiae and Saccharomyces paradoxus

The paper analyzes RNAseq data from yeast species to study how regulatory networks controlling gene expression plasticity evolve in response to environmental changes.

Deciphering subcellular localization-dependent functions of Hog1 MAPK in Cryptococcus neoformans

This study shows that Hog1 MAPK localization in Cryptococcus neoformans determines stress adaptation, antifungal resistance, and virulence by affecting glycerol homeostasis and sexual differentiation.

Dynamic Reprogramming of Fungal Cell Walls Underlies Germination and Immune Exposure in Zygomycetous Fungal Pathogens

This study shows how the cell wall of Rhizopus delemar reprograms during germination, enhancing immune recognition, and identifies key polysaccharide changes involved.

Mating-type locus structure affects gene expression in unidirectional mating-type switching fungi

The paper shows that variations in the MAT1 locus structure in fungi affect gene expression beyond the sexual cycle, including genes unrelated to mating.

On the evolution, function and cellular fate of Neurospora crassa ACW-1 and NCW-3, proteins with different cell wall interaction mechanism

This study compares the evolutionary and functional roles of two Neurospora crassa cell wall proteins, ACW-1 and NCW-3, revealing differences in their sequence diversification patterns despite similar global evolutionary constraints.

Differential tolerance for SEA domain misfolding encodes a MAPK pathway-specific response

This paper identifies differential tolerance for SEA domain misfolding in two MAPK pathways, revealing distinct signaling mechanisms that confer pathway-specific responses to environmental stress.

Environmental Regulation and Gene-by-Environment Interaction Influence RAP1 Activity and its Impact on Gene Expression

This study investigates how RAP1 activity and gene-by-environment interactions affect gene expression in yeast across different growth conditions.

Effects of Chitosan as a Permeabilizing Agent in Different Yeast Species. Studying Enzymes in situ.

This study examines chitosan's permeabilizing effects on three yeast species, showing strain-dependent antifungal activity and its utility for in situ enzyme activity measurement.

Structural evolution of a fungal cell wall protein family for β-glucan-binding and cell separation

This study determines the structure and function of fungal SUN domain proteins, revealing their role in β-glucan binding and cell separation through conserved residues and domain fusion.

High-resolution, genotype-free mapping of genetic variation with CRI-SPA-Map

The paper introduces CRI-SPA-Map, a genetic mapping strategy in yeast that identifies causal genetic variants for phenotypic differences without genotyping.

Vsb1, Ypq1 and Ypq2 control dynamic cationic amino acid storage in the yeast vacuole

Zaremba et al. characterize Vsb1 as the main yeast vacuolar lysine importer and Ypq1/2 as bidirectional vacuolar transporters of lysine and arginine. Their study highlights the role of vacuolar transporters in regulating cationic amino acid homeostasis under fluctuating nutrient availability.

A conserved Pho4-Pho84 axis ubiquitously maintains intracellular phosphate homeostasis in yeast

The paper identifies Pho4-Pho84 as a conserved mechanism for maintaining intracellular phosphate homeostasis in yeast under phosphate-replete conditions.

Mechanosensation Promotes Local Cell Wall Repair

This study shows that mechanosensors detect cell wall defects in fission yeast, triggering polarity regulators to repair the wall.

Gene loss, repression, amplification, and horizontal acquisition shape galactose/melibiose metabolism in fission yeast

The paper examines natural variation in galactose and melibiose metabolism in fission yeast, identifying gene loss, repression, amplification, and horizontal gene transfer as mechanisms shaping metabolic diversity.

Yeast MoClo secretion and surface display toolkit 2.0: improvements and applications for analysis of protein-protein interactions and whole-cell biocatalysis

The paper improves and applies a yeast secretion and display toolkit for analyzing protein interactions and whole-cell biocatalysis.

High extracellular osmolarity promotes yeast thermotolerance through osmotic modulation and glycerol-dependent adaptation.

This study shows that high extracellular osmolarity improves yeast thermotolerance by modulating osmotic balance and enhancing glycerol accumulation under heat stress.

Functional characterisation of an essential neo-chromosome III in Sc2.0 strain reveals opportunities and challenges for genome minimisation in Sc3.0

The paper engineers an essential neo-chromosome III in yeast to enable genome minimisation by relocating essential genes, demonstrating stability and cross-species complementation.

Met32 governs transcriptional control of sulfur metabolic flexibility and resistance to reactive sulfur species in the human fungal pathogen Candida albicans

This study identifies Met32 as a master regulator of sulfur utilization and resistance to reactive sulfur species in C. albicans, highlighting its role in fungal virulence.

High-throughput screening reveals mechanisms of environmental control of germination in a fungal thermophile

This study identifies genetic mechanisms controlling temperature-dependent germination in the fungal thermophile Thermothelomyces thermophilus through insertional mutagenesis and validation of zinc transporter role.

The pangenome of Aspergillus fumigatus highlights the dynamics of gene gain-loss over evolutionary timescales in a human fungal pathogen

This paper quantifies gene gain and loss dynamics in Aspergillus fumigatus over a century using a global pangenome, revealing slow genome evolution and lineage-specific resistance mechanisms.

A MAP kinase cascade modulates expression of late G1 phase cyclins in budding yeast

This study identifies a TORC2-Pkc1-MAP kinase signaling pathway that modulates Cln2 protein expression in budding yeast, linking nutrient availability to bud growth.