Mycobacterium research and diagnosis

This study shows that combinations of dual β-lactams and β-lactamase inhibitors synergistically inhibit Mycobacterium abscessus growth, challenging the traditional view of their mechanisms.

This study develops a humanized lung-on-a-chip model to study early immune responses to Mycobacterium fortuitum infection, revealing cytokine and protease inhibitor upregulation.

This study compares the efficacy of DAR-901 and BCG vaccines in inducing immunity against Mycobacterium avium in mice, showing similar cross-reactive immunity and protection against aerosolized MAC infection.

This study evaluates the in vitro activity of imipenem/relebactam alone and in combination with other antibiotics against M. abscessus isolates from cystic fibrosis patients, finding synergistic and additive effects with several drugs.

This study investigates the interaction between Mycobacterium abscessus and Pseudomonas aeruginosa in coinfection, showing that M. abscessus inhibits immune responses, worsening infection outcomes.

The paper identifies membrane permeability and efflux pumps as key mechanisms of intrinsic antibiotic resistance in Mycobacterium abscessus and highlights potential targets for improving antibiotic efficacy.

This study identifies M cells as the primary target of Mycobacterium avium subspecies paratuberculosis (MAP) entry into the small intestinal epithelium, mediated by fibronectin-bridging interactions with β1 integrins.

The paper tests triple antimicrobial combinations against M. abscessus isolates and finds synergistic activity in several combinations, with some sensitizing resistant isolates.

The paper evaluates antibiotic regimens in a mouse model of M. avium infection, finding that a triple-drug regimen is more effective than monotherapy.

This paper develops a subunit vaccine, Burulivac, that fully protects mice against Buruli ulcer in a footpad model by inducing protective immune responses.

This study shows that disrupting MtrA or MtrB in Mycobacterium abscessus increases antibiotic susceptibility and reduces virulence by affecting cell division and permeability.

This study investigates Buruli ulcer in possums in Victoria, Australia, finding M. ulcerans in 78% of clinically affected and 65% of non-lesioned possums, with lesions mapped and systemic bacterial dissemination observed.

This study identifies 21 NTM species in suspected tuberculosis patients in Iran, highlighting significant genetic diversity in M. kansasii, M. gordonae, and M. abscessus.

The paper demonstrates the use of dual host-bacterial gene expression analysis in FFPE tissues to study pathogenesis and virulence factor regulation during respiratory infections.

This study identifies genetic changes in M. abscessus outbreak strains that lead to high imipenem resistance, suggesting carbapenem/{beta}-lactamase inhibitor combinations for treatment.

The paper develops a low-cost DNA extraction method using paramagnetic beads for environmental surveillance of Mycobacterium ulcerans in possum excreta and tissue swabs, showing cost savings and comparable DNA quality to commercial kits.

This study identifies EmbB and EmbC as regulators of Mycobacterium abscessus sensitivity to echinomycin, showing that EmbB mutations can partially compensate for EmbC loss and alter cell wall permeability.

This study examines the relationship between morphology, glycopeptidolipids, and infectivity in Mycobacterium abscessus clinical isolates using macrophage models.

This study investigates NTM occurrence in a chloraminated drinking water system, finding higher concentrations in summer first draw samples and highlighting the role of flushing and building plumbing in NTM persistence.

This study analyzes 665 Mycobacterium kansasii complex strains to identify unique and shared genomic signatures, revealing lineage splits, virulence factors, and drug resistance mechanisms.

This study shows that sulopenem combined with cefuroxime or durlobactam synergistically inhibits Mycobacterium abscessus, with BLIs enhancing antibacterial effects.

The paper identifies strain-specific genetic requirements for hypoxic growth in Mycobacterium intracellulare using transposon sequencing and validates findings in a mouse infection model.

This paper identifies M. ulcerans JKD8049 as a suitable human challenge strain for Buruli ulcer research due to its stability, antibiotic susceptibility, and ability to produce mycolactone.

This study evaluates the in vitro bactericidal activity of amoxicillin/clavulanate combined with rifampicin/clarithromycin against Mycobacterium ulcerans and compares methodologies for bacterial load quantification.

This study identifies Lsr2 as a pleiotropic transcription factor regulating gene expression and antibiotic resistance in Mycobacterium abscessus morphotypes using RNA-seq and ChIP-seq.

The paper shows that phylogenetic clustering in Mycobacterium abscessus is linked to reduced mutation rates and DNA repair gene variation, suggesting adaptation rather than transmission as the cause.

This study presents the genome of M. brumae and shows its nonpathogenic and immunogenic traits through genomic analysis.

The paper characterizes a new prophage cluster, MabR, in Mycobacterium abscessus and Mycobacterium chelonae, showing its role in antibiotic resistance and virulence.

This study identifies mutations in Mycobacterium ulcerans DNA gyrase that reduce susceptibility to fluoroquinolones in vitro.