Mycobacterium research and diagnosis

Does Infection Progression of Mycobacterium abscessus Depend on Sex, Age, and Mouse strain?

This study evaluates how sex, age, and mouse strain affect M. abscessus infection progression using a beads model, finding BALB/cJRJ mice show more sustained infection with less variability.

Carbon starvation of Mycobacterium abscessus induces a non-replicating state with extensive proteomic remodeling

This study identifies extensive proteomic changes in Mycobacterium abscessus under carbon starvation, highlighting the role of the DosRS system in non-replicating persistence.

Targeting homologous recombination repair to potentiate fluoroquinolone efficacy in Mycobacterium abscessus

This study identifies homologous recombination as a key mechanism of fluoroquinolone resistance in Mycobacterium abscessus and shows that targeting HR enhances FQ efficacy in vitro and in vivo.

Clinical isolates of Mycobacterium avium complex reveal an M. intracellulare-associated IL-17/neutrophilic pulmonary immune program in a murine disease model

The paper investigates immune responses to different Mycobacterium avium complex isolates in mice, finding that M. intracellulare induces a distinct IL-17/neutrophilic immune program compared to M. avium.

Tobramycin enhances Mycobacterium abscessus fitness through whiB7 induction

This paper shows tobramycin increases Mycobacterium abscessus survival by inducing whiB7, which enhances resistance to oxidative stress and macrophage killing.

Identification and characterization of ethR and ethA genes impacting the sensitivity of Mycobacterium abscessus to ethionamide

This study identifies EthR and EthA genes in Mycobacterium abscessus that mediate resistance to ethionamide, showing EthR is a more dominant resistance factor than NudC.

Expanding the scope of Mycobacterium abscessus reference strains to improve pulmonary disease modeling

This study identifies Mab clinical isolates that better model human pulmonary disease compared to the commonly used ATCC 19977 strain in mouse models.

NTM-DB: A Comprehensive Non-Tuberculosis Mycobacteria Genomic Database

NTM-DB provides a comprehensive genomic database for non-tuberculosis mycobacteria, offering genome assemblies, strain data, and analysis tools to support research and clinical applications.

The stress response factor SigH mediates intrinsic resistance to multiple antibiotics in Mycobacterium abscessus

This study identifies SigH as a key regulator of intrinsic antibiotic resistance in Mycobacterium abscessus through transcriptomic analysis and genetic complementation.

Diagnostic Value of Antibody Responses to Mycobacterium avium subsp. paratuberculosis-Derived Proteins PtpA and PtpB in Rheumatoid Arthritis

This study assesses anti-PtpA and anti-PtpB antibody levels as biomarkers for rheumatoid arthritis, finding higher levels in RA patients and improved diagnostic accuracy with a combined model.

Chemoproteomic elucidation of β-lactam drug targets in Mycobacterium abscessus

This study identifies β-lactam targets in Mycobacterium abscessus using chemoproteomics, revealing 17 conserved targets and demonstrating imipenem's superior inhibition over cefoxitin.

Sulbactam-Durlobactam Plus Ceftriaxone Dosing and Novel Treatment Regimens for Mycobacterium abscessus Lung Disease

This study evaluates sulbactam-durlobactam plus ceftriaxone dosing and combination regimens for Mycobacterium abscessus lung disease, finding that sulbactam-durlobactam-ceftriaxone achieves the highest microbial kill in a hollow fiber model.

Antibiotic dose-response curves can measure antibiotic activity against Mycobacterium abscessus and Mycobacterium peregrinum

This study uses dose-response curves to measure antibiotic activity against Mycobacterium abscessus and Mycobacterium peregrinum, showing that IC50 and IC75 are more consistent than MIC.

Strain-dependent disease progression and necrotizing granuloma formation induced by virulent Mycobacterium avium complex strains in a murine model

This study evaluates strain-dependent disease progression and necrotizing granuloma formation in a murine model of MAC-PD using five clinical MAC strains.

Mutations in the ubiA gene are the major mechanism of ethambutol resistance in Mycobacterium avium

This study identifies ubiA gene mutations as the primary mechanism of ethambutol resistance in M. avium by analyzing clinical strains and resistant mutants.

Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessus

This study identifies an ABC-F protein in Mycobacterium abscessus that confers resistance to linezolid and other ribosome-targeting antibiotics.

Genetic analysis of Mycobacterium abscessus reveals genomic diversity linked to global plasmid distribution

The paper analyzes plasmid distribution in Mycobacterium abscessus using whole genome sequencing to show how plasmid transfer increases genomic diversity and aids in estimating bacterial contact history.

A division-associated envelope protein, MAB_2363, drives intrinsic resistance and virulence in Mycobacterium abscessus

This study identifies MAB_2363 as a division-associated protein that contributes to intrinsic resistance and virulence in Mycobacterium abscessus by affecting cell division and antibiotic susceptibility.

Lower Airway Dysbiosis in NTM+ Bronchiectasis is Associated with NET-Predominant Severe Phenotypes

This study links lower airway microbiota and neutrophilic inflammation in NTM+ bronchiectasis to severe disease phenotypes through network analysis and preclinical models.

Efficacies of sequenced monotherapies of Mycobacterium avium lung infection in mouse

This study evaluates sequential monotherapy for M. avium lung infection in mice, finding it as effective as multidrug therapy without promoting resistance.

A cross-environment comparison of nontuberculous mycobacterial diversity

This study compares nontuberculous mycobacterial diversity across surface waters, soil, and household plumbing biofilms, finding that plumbing biofilms have the highest abundance and occurrence of clinically relevant species.

Mycobacterial cell division arrest and smooth-to-rough envelope transition using CRISPRi-mediated genetic repression systems

This paper describes a CRISPRi-based protocol to assess gene silencing and envelope transitions in Mycobacterium smegmatis as a model for NTM pathogenesis.

Primary tuberculous mycobacterial granulomas provide a niche for superinfecting Mycobacterium abscessus

The paper shows that Mycobacterium abscessus grows preferentially in granulomas formed by prior Mycobacterium marinum infection in zebrafish, exploiting a nutrient-rich niche.

Distribution, Prevalence of Non-Tuberculous Mycobacteria in Hainan Island and Antibiotic Resistance of Mycobacterium abscessus

This study maps NTM distribution and antibiotic resistance in Hainan Island, identifying key species and resistance patterns to inform regional surveillance and treatment.

Defining new Buruli ulcer endemic areas in urban southeastern Australia using bacterial genomics-informed possum excreta surveys

Tigecycline pharmacodynamics in the hollow fiber system of Mycobacterium avium-complex lung disease, and the utility of MICs and time-kill studies in drug development

The paper evaluates tigecycline pharmacodynamics in MAC lung disease using time-kill studies and hollow fiber systems, finding EC50 values and suggesting inhaled tigecycline as a potential treatment.

Enhanced Activity of Apramycin and Apramycin-Based Combinations Against Mycobacteroides abscessus

This study compares the in vitro activity of apramycin and other aminoglycosides against Mycobacteroides abscessus and related species, finding apramycin more potent than amikacin.

A multiplexed, target-based phenotypic screening platform using CRISPR interference in Mycobacterium abscessus

This paper introduces a CRISPRi-based PROSPECT platform for drug discovery in M. abscessus, identifying new compound targets and revealing unexpected susceptibility to isoniazid.

GplR1, an unusual TetR-like transcription factor in Mycobacterium abscessus, controls the production of cell wall glycopeptidolipids, colony morphology, and virulence

This paper identifies GplR1, a transcription factor in Mycobacterium abscessus, as a regulator of glycopeptidolipid production, colony morphology, and virulence.

A refined low-dose murine model of Mycobacterium ulcerans infection to assess integrated immune networks in Buruli ulcer pathogenesis

This study develops a low-dose murine model of Mycobacterium ulcerans infection to analyze immune responses and identifies key cell types involved in disease progression.