Mycobacterium research and diagnosis

Application of the ORBIT Protocol for Targeted Gene Deletion to Mycobacterium abscessus

This paper optimizes the ORBIT protocol for efficient gene deletion in Mycobacterium abscessus, enabling precise genetic manipulation for studying its physiology and pathogenesis.

Modelling the formation of skin ulcers observed in Buruli ulcer disease

This paper develops a mathematical model to study the dynamics of Buruli ulcer formation, showing that mycolactone concentration and bacterial diffusion rates influence wound expansion speed.

Reduced recombination relative to mutation characterizes dominant circulating clones of Mycobacterium abscessus

The paper analyzes genomic diversity in Mycobacterium abscessus, finding that dominant clones show increased accessory-gene gains and reduced recombination relative to mutation after establishment.

Parallel Emergence and Adaptive Evolution of Multicountry Circulating Mycobacterium abscessus Clones

The paper analyzes global M. abscessus genomes to show that recently emerged clones spread across countries and show shifts in selective pressures during expansion.

Selected clinical strains of Mycobacterium avium unlock in vivo models for NTM drug discovery programs

The paper establishes a clinically relevant M. avium model using clinical isolates and longitudinal imaging to evaluate anti-mycobacterial drugs in vivo.

Emergence of plasmid-borne erm(55)-associated macrolide resistance in Mycobacterium chelonae and other rapidly-growing non-tuberculous mycobacteria in Europe

The paper identifies plasmid-borne erm(55) macrolide resistance in M. chelonae and other RGM in Europe, showing inducible resistance and genetic similarity to known plasmids.

Transposon insertion sequencing identifies genetic determinants of intrinsic rifamycin resistance in Mycobacterium abscessus

This study uses transposon insertion sequencing to identify genes contributing to intrinsic rifamycin resistance in Mycobacterium abscessus, revealing new mechanisms and potential therapeutic strategies.

Analysis of publicly available genomic data

This study identifies genomic mutations associated with ethambutol resistance in Mycobacterium avium-intracellulare using clinical isolates and publicly available genomic data.

First characterization of Mycobacterium avium subsp. paratuberculosis strain types in cattle from Kushiro Subprefecture, eastern Hokkaido, Japan

The study characterizes Mycobacterium avium subsp. paratuberculosis strain types in cattle from Japan, finding no Type S strains and suggesting prevalence of Type C strains.

A Murine Model of Mycobacterium abscessus Encapsulated in Alginate-Beads: Advancing Toward a Chronic Infection Model

This paper develops a murine model of Mycobacterium abscessus infection using alginate-encapsulated bacteria, showing slower clearance and increased dissemination, and evaluates antibiotic efficacy against encapsulated versus free bacteria.

Novel regimens for treatment of Mycobacterium avium lung disease based on advanced in vitro systems and the mathematics of basis functions

The paper evaluates new drug regimens for Mycobacterium avium lung disease using an in vitro model and basis functions, finding that ceftriaxone-omadacycline-rifabutin outperforms the standard-of-care.

Alpha-1-antitrypsin (AAT) inhibits Mycobacterium intracellulareinduction of monocyte colony stimulating factor: another host defense function of AAT

This study shows AAT inhibits MAC-induced M-CSF but not GM-CSF in macrophages, suggesting a role in modulating host defense against mycobacteria.

Ecological Drivers of Nontuberculous Mycobacteria in Aquatic Systems: Biodiversity, Niche Competition, and Pathogen Emergence

The paper investigates ecological factors driving nontuberculous mycobacteria in aquatic systems, finding habitat type, abiotic factors, and microbial interactions influence their distribution and disease transmission.

A highly conserved two-gene operon is crucial for lipoarabinomannan localization, pathogenesis, and cell envelope function in Mycobacterium abscessus

This study identifies the lprg-mfs operon as essential for virulence, lipid distribution, and cell envelope integrity in Mycobacterium abscessus.

A mobile ESX type VII secretion system enhances intracellular persistence in globally distributed Mycobacterium abscessus

This study identifies a novel plasmid-borne ESX type VII secretion system in M. abscessus that enhances intracellular survival in macrophages.

Species Identification and Antimicrobial Susceptibility of Mycobacterial Isolates from Dogs and Cats

This study identifies NTM species and their antimicrobial resistance in cats and dogs, finding rapid growers dominate and resistance varies by species.

Concentration-dependent suppression of macrolide resistance in Mycobacterium avium by combination therapy: an improved in vitro time-kill assay and PK/PD modelling study

This study quantifies how combination therapy with concomitant drugs suppresses macrolide resistance in M. avium using improved time-kill assays and PK/PD modeling.

CRISPRi-mediated validation of candidate Myocobacterium abscessus drug targets during host infection

This study uses CRISPRi to validate four candidate drug targets in Mycobacterium abscessus during infection, showing varying in vivo efficacy depending on host immune status.

A bioluminescence-based chemical screen identifies a bactericidal naphthalene scaffold targeting MmpL3 in Mycobacterium abscessus

Integrated analysis of Tn-Seq and pangenome reveals core and lineage-specific essential genes in Mycobacterium avium subsp. hominissuis.

The paper integrates Tn-Seq and pan-genome analysis to compare essential genes across MAH strains, finding core and lineage-specific essential genes.

Plasmid-mediated macrolide resistance among rapidly growing mycobacteria in Japan

This study identifies a low prevalence of erm(55)-carrying plasmids in Japanese RGM isolates, conferring inducible macrolide resistance.

From colonisation to chronicity: adaptation of Mycobacterium abscessus in the cystic fibrosis lung environment

This study examines how Mycobacterium abscessus adapts during chronic infection in the CF lung, showing increased host cell attachment and intracellular survival in late isolates.

REBASTINIB, OLVEREMBATINIB, AND NAS-181 INHIBIT MYCOBACTERIUM ABSCESSUS BY DISRUPTING MYCOTHIOL HOMEOSTASIS

This study identifies rebastinib, olverembatinib, and NAS-181 as potential drugs that inhibit Mycobacterium abscessus by disrupting mycothiol homeostasis, showing efficacy in vitro and in vivo.

An unusual genetic switch controls Mycobacterium avium pathogenesis, antibiotic resistance and colony morphology

This study identifies a genetic switch involving IS1245 transposition and Erp/MarP that controls colony morphology, virulence, and antibiotic resistance in Mycobacterium avium.

Systematic and quantitative analyses of pre-clinical Mycobacterium avium Lung Disease Tools for Drug Development and Transition from Animal Models to New Approach Methodologies

The paper benchmarks preclinical tools for Mycobacterium avium lung disease drug development, finding significant variability in PK/PD target attainment and recommending HFS-MAC for antibiotic ranking and translational studies.

Divergent MIC of Clarithromycin and Azithromycin Against Clinical Isolates of Mycobacterium abscessus Complex

This study compares the in vitro activity of clarithromycin and azithromycin against M. abscessus complex isolates, finding significant resistance differences and discordance in susceptibility profiles.

Divergent Minimum Inhibitory Concentrations of Clarithromycin and Azithromycin Against Clinical Isolates of Mycobacterium abscessus Complex

This study compares the in vitro activity of clarithromycin and azithromycin against M. abscessus complex isolates, finding significant resistance differences and discordance in susceptibility profiles.

Transcriptional Regulator, Metabolic, and Pilus Biosynthesis Genes as Candidate Virulence Markers in High-Virulence Mycobacterium abscessus

This study identifies 20 conserved genes in clinical Mycobacterium abscessus isolates that are absent in environmental isolates, suggesting they may contribute to virulence and pathogenicity.

Leveraging the subgenus category to address genus over-splitting exemplified with Prescottella and other recently proposed Mycobacteriales genera.

The paper proposes using the subgenus category to resolve genus over-splitting in prokaryotic taxonomy, exemplified with Prescottella and Mycobacterium splits.

Identification of Novel Mutations Causing Clofazimine Resistance in Mycobacterium intracellulare

This study identifies mutations in the marR gene and other genes associated with clofazimine resistance in Mycobacterium intracellulare through whole-genome sequencing and complementation experiments.