Advanced NMR Techniques and Applications

NMR Spectral Alignment Utilizing a CryoEM Motion Correction Algorithm

This paper introduces ANSA, an automated NMR spectral alignment tool using cryo-EM motion correction, improving alignment accuracy and reducing manual effort in NMR data processing.

Homonuclear Chemical Shift Correlation in Solids Under MAS by Fast Cross-Relaxation Driven Spin Diffusion

This paper introduces a 2D NMR technique for homonuclear chemical shift correlation in solids under MAS, showing rapid spin-exchange between nearby 15N or 13C nuclei.

Multiplexed analysis of multicomponent biomolecular condensates without any tag

This paper introduces a non-invasive NMR method to study multiple components in biomolecular condensates without tags, demonstrating its effectiveness with FUS and hnRNPC1 samples.

In-cell residue-resolved NMR of micromolar α-synuclein and tau at 310K

This study uses in-cell NMR at 310K to analyze α-synuclein and tau conformations and interactions in live human cells at micromolar concentrations.

Signal-Level Witnessing of SU(1,1) Pair Dynamics in Brain Proton Spin Ensembles

The paper identifies SU(1,1) pair dynamics in brain proton spin ensembles using a symmetry-based framework and reanalysis of magnetic-resonance data.

Towards site-specific information on PET degrading enzymesusing NMR near operational temperature

This paper improves NMR spectroscopy for studying PET-degrading enzymes by recording spectra at higher temperatures, enhancing signal-to-noise ratios and enabling faster backbone assignment.

In Situ Structure Determination of a Membrane Protein in Native Cellular Membranes by Proton-Detected Solid-State NMR

This paper uses proton-detected solid-state NMR to determine the high-resolution structure of MscL in native E. coli membranes, achieving better sensitivity than 13C-detected methods.

Oral Intake of Deuterated Choline at Clinical Dose for Metabolic Imaging of Brain Tumors

This study investigates oral administration of deuterated choline for metabolic imaging of brain tumors, showing comparable tumor contrast to intravenous administration using a clinically relevant dose.

Selective Detection of a Key Region in Chemotaxis Signaling Protein Complexes by Solid-State NMR

This paper uses solid-state NMR to selectively detect rigid regions in chemotaxis signaling protein complexes, revealing conformational changes between signaling states.

A Simple Algorithm to Suppress Diagonal Peaks in High-Resolution Homonuclear Chemical Shift Correlation NMR Spectra

The paper introduces a spectral shearing method to suppress diagonal peaks in NMR spectra while preserving cross peak sensitivity.

Deconvoluting Metabolomic Flux in Living Cells with Real-time 13C J-coupling-edited Proton High-Resolution Magic Angle Spinning (HRMAS) NMR

This paper introduces a 1H NMR method to enhance 13C signal detection for studying cellular metabolism in low-volume samples, demonstrating its application in tracking metabolic reactions in Clostridioides difficile.

NMRFx: Integrated Software for NMR Data Processing, Visualization, Analysis and Structure Calculation

NMRFx is a new software tool that integrates NMR data processing, visualization, analysis, and structure calculation, with case studies demonstrating its application in various molecular systems.

Pushing Sensitivity and Specificity Limits in Native Structural Biology: 19F Multinuclear Dynamic Nuclear Polarization with Magic Angle Spinning

The paper introduces a 19F-based DNP MAS NMR method for atomic-level structural analysis of proteins in mammalian cells, demonstrating high sensitivity and specificity for detecting target proteins.

Backbone Assignment of a 28.5 kDa Class A Extended Spectrum β-Lactamase by High-Field, Carbon-Detected Solid-State NMR

The paper reports high-resolution 13C and 15N backbone chemical shift assignments for Toho-1 β-lactamase using advanced solid-state NMR techniques, confirming conserved secondary structure.

Quantification of small molecule partitioning in a biomolecular condensate with 2D NMR spectroscopy

The paper demonstrates a method to quantify small molecule partitioning between biomolecular condensate phases using 2D NMR spectroscopy without isolating components.

Ultra-High Resolution Solid-State NMR for High Molecular Weight Proteins on GHz-Class Spectrometers

This study improves solid-state NMR resolution for high molecular weight proteins using GHz-class spectrometers and novel techniques, achieving better than 0.2 ppm resolution for proteins up to 144 kDa.

Measure Sodium Transport in Cells with NMR

This study introduces a non-invasive NMR method to measure sodium ion transport in living cells, showing its ability to distinguish physiological states and correlate with metabolic activity.

Probing site-specific RNA dynamics by solid-state NMR spectroscopy

This study uses solid-state NMR to analyze site-specific RNA dynamics through 15N T1 relaxation times, revealing correlations with structural elements.

Backbone and Sidechain 1H, 15N and 13C Resonance Assignments of a Multidrug Efflux Membrane Protein using Solution and Solid-State NMR

The paper reports NMR resonance assignments for EmrE, a multidrug transporter, using solution and solid-state NMR techniques at specific pH conditions.

ChipNMR: Hyperpolarized NMR for non-invasive metabolic flux analysis in perfused microfluidic chips

This paper introduces a microfluidic chip integrated with an NMR probe for real-time metabolic flux analysis of adherent cells, demonstrating its use with HeLa cells over 48 hours.

Autonomous adaptive optimization of NMR experimental conditions for precise inference of minor conformational states of proteins based on chemical exchange saturation transfer

The paper develops an adaptive optimization system for NMR CEST experiments to improve estimation of minor protein conformational states using Bayesian methods and MCMC.

Rational design of 19F NMR labelling sites to probe protein structure and interactions

This paper designs 19F NMR labels to improve chemical shift dispersion and enable structural analysis of proteins and their interactions.

Proton-Detected Solid-State NMR for Deciphering Structural Polymorphism and Dynamic Heterogeneity of Cellular Carbohydrates in Pathogenic Fungi

Targeted Molecular MRI of Colorectal Cancer by Antibody Functionalized Hyperpolarized Silicon Particles

This study develops hyperpolarized silicon particles functionalized with MUC1 antibody for targeted molecular MRI of colorectal cancer, showing active tumor targeting in vivo.

In situ light-driven pH modulation for NMR studies

This paper introduces a method to control pH in NMR samples using light-activated photoacids, enabling non-invasive and precise pH modulation for studying protonation states in proteins.

Mycobacterium tuberculosis CrgA Forms a Dimeric Structure with Its Transmembrane Domain Sandwiched between Cytoplasmic and Periplasmic β-Sheets, Enabling Multiple Interactions with Other Divisome Proteins

This study determines the structure of Mycobacterium tuberculosis CrgA, revealing its dimeric form and interactions with divisome proteins essential for cell division.

Exploring the Biochemical Landscape of Bacterial Medium with Pyruvate as the Exclusive Carbon Source for NMR Studies

This study investigates E. coli metabolism on pyruvate as the sole carbon source for NMR studies, revealing metabolic inefficiencies that impact isotopic labeling.

Counter-intuitive method improves yields of isotopically-labeled proteins expressed in flask-cultured Escherichia coli

The paper develops a cost-effective method for producing isotopically-labeled proteins in E. coli by optimizing nutrient use and reducing 13C-D-glucose consumption.

Quantitative Interpretation of Transverse Spin Relaxation by Translational Diffusion in Liquids Under Arbitrary Potentials

The paper provides a rigorous theoretical interpretation of the transverse spin relaxation rate {Gamma} 2, showing it is proportional to norm and offers an explicit formula for calculating norm without adjustable parameters.

Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding

This paper introduces a Hadamard encoding method for multi-dimensional solid-state NMR spectral editing of protein sidechains, enabling unambiguous detection of signals from basic amino acids and their bound water molecules.