Advanced NMR Techniques and Applications

Validated backbone assignments for natural-abundance 1H,15N spectra of commercial hen egg white lysozyme and example applications thereof

The paper provides validated 1H,15N backbone assignments for commercial hen egg white lysozyme at natural 15N abundance and demonstrates their utility in various applications.

Methyl-TROSY NMR at 1.2 GHz: exploiting resolution and magnetic field alignment for structure and dynamics of large biomolecular assemblies

This study evaluates methyl-TROSY NMR at 1.2 GHz for analyzing structure and dynamics of large biomolecular assemblies, showing improved resolution and insights into nucleosome DNA unwrapping.

Hypersensitive Detection of Neurotransmitters in Biological Media by Optically Enhanced Benchtop NMR Spectroscopy

This paper introduces optically enhanced NMR techniques to significantly improve sensitivity for detecting neurotransmitters in biological samples.

Efficient Through-Bond Propagation of Nuclear-Spin Hyperpolarization via TOCSY-Enhanced LC-Photo-CIDNP

This paper introduces a method to enhance NMR sensitivity by propagating hyperpolarization through molecular bonds using TOCSY and LC-photo-CIDNP, enabling visualization of low-concentration samples.

Expanding the Frontiers of Structural Analysis in Short RNAs by Ultra-High Field 1.3 GHz NMR

This paper demonstrates the first media-free detection of field-induced RDCs in a short RNA using 1.3 GHz NMR, showing structural insights and unique chemical shift anisotropy effects.

Makeshift: a lightweight software for accessing and analyzing NMR data and protein dynamics

Makeshift provides a lightweight Python package for accessing and analyzing NMR data, enabling reproducible and scalable analysis of biomolecular dynamics and chemical environments.

Integrated solid/solution NMR assignment allows mapping dynamics and ligand binding in a 134 kDa enzyme

This paper uses integrated solid and solution NMR to study dynamics and ligand binding in a 134 kDa enzyme, revealing structural flexibility and substrate interactions.

Nanosecond methyl dynamics in the eukaryotic RNA exosome core

This study uses NMR to measure nanosecond methyl dynamics in the RNA exosome core, showing that relaxation-violated coherence transfer experiments can quantify side chain order parameters in large protein complexes.

19F Ultrafast MAS NMR Reveals the Dynamic Basis of pH-Dependent Regulation in Proteorhodopsin

This paper uses 19F ultrafast MAS NMR to study pH-dependent dynamics in proteorhodopsin, revealing the role of specific residues in proton transport and photocycle regulation.

A deployable microfluidic platform for parallel and longitudinal hyperpolarised magnetic resonance metabolic phenotyping

This paper introduces a microfluidic platform that enables parallel and longitudinal hyperpolarised metabolic phenotyping using standard MRI, increasing throughput and reducing sample preparation.

Structure-activity of outer membrane proteins in native bacterial membrane vesicles by solid-state NMR

This paper uses solid-state NMR to analyze the structure and activity of outer membrane proteins in native bacterial vesicles, showing their functionality and interactions with host ligands.

Accelerated Measurement of Chemical Exchange Saturation Transfer by Accordion NMR Spectroscopy

This paper introduces ACCEST, a method that accelerates chemical exchange saturation transfer (CEST) measurements by using a single two-dimensional accordion-CEST spectrum, reducing experiment time significantly.

CcpNmr AnalysisDynamics: a unified framework for NMR dynamics data analysis

The paper introduces CcpNmr AnalysisDynamics, a unified framework for analyzing NMR relaxation data, enabling reproducible workflows and model-based interpretation of biomolecular dynamics.

Facilitating NMR Resonance Assignment with Metabolic Tampering

This paper introduces a method to improve NMR resonance assignment by modulating metabolic flux during protein expression, using 15N-enriched media with natural abundance media to create unique peak patterns.

The NMR Exchange Format (NEF): Specification and Applications

The paper introduces NEF, a standard for NMR data exchange, and demonstrates its application in structure determination, metabolomics, and ligand screening.

Comparison of HDO production from Glucose as a marker of Glucose metabolism

The paper compares HDO production from two glucose isotopes in vitro and in vivo, finding [2,3,4,6,6-2H5]glucose produces comparable HDO to [2H7]glucose with lower cost and simpler spectra.

Higher Magnetic Field NMR Renders Resolution Enhancement on Ganglioside GD3 Catalyzed Abeta42 Aggregates

This study shows that high-field SSNMR improves resolution and sensitivity for analyzing heterogeneous Aβ42 aggregates catalyzed by GD3.

Chemical control of CSA geometry enables relaxation-optimized 19F-13C NMR probes

This paper demonstrates how controlling the CSA tensor geometry in 19F-13C NMR probes can suppress transverse relaxation, enabling optimized probes for large biomolecular systems.

Homonuclear Chemical Shift Correlation in Solids Under MAS by Fast Cross-Relaxation Driven Spin Diffusion

This paper introduces a 2D NMR technique for homonuclear chemical shift correlation in solids under MAS, showing rapid spin-exchange between nearby 15N or 13C nuclei.

Signal-Level Witnessing of SU(1,1) Pair Dynamics in Brain Proton Spin Ensembles

The paper identifies SU(1,1) pair dynamics in brain proton spin ensembles using a symmetry-based framework and reanalysis of magnetic-resonance data.

In Situ Structure Determination of a Membrane Protein in Native Cellular Membranes by Proton-Detected Solid-State NMR

This paper uses proton-detected solid-state NMR to determine the high-resolution structure of MscL in native E. coli membranes, achieving better sensitivity than 13C-detected methods.

Deconvoluting Metabolomic Flux in Living Cells with Real-time 13C J-coupling-edited Proton High-Resolution Magic Angle Spinning (HRMAS) NMR

This paper introduces a 1H NMR method to enhance 13C signal detection for studying cellular metabolism in low-volume samples, demonstrating its application in tracking metabolic reactions in Clostridioides difficile.

NMRFx: Integrated Software for NMR Data Processing, Visualization, Analysis and Structure Calculation

NMRFx is a new software tool that integrates NMR data processing, visualization, analysis, and structure calculation, with case studies demonstrating its application in various molecular systems.

Measure Sodium Transport in Cells with NMR

This study introduces a non-invasive NMR method to measure sodium ion transport in living cells, showing its ability to distinguish physiological states and correlate with metabolic activity.

Probing site-specific RNA dynamics by solid-state NMR spectroscopy

This study uses solid-state NMR to analyze site-specific RNA dynamics through 15N T1 relaxation times, revealing correlations with structural elements.

ChipNMR: Hyperpolarized NMR for non-invasive metabolic flux analysis in perfused microfluidic chips

This paper introduces a microfluidic chip integrated with an NMR probe for real-time metabolic flux analysis of adherent cells, demonstrating its use with HeLa cells over 48 hours.

Targeted Molecular MRI of Colorectal Cancer by Antibody Functionalized Hyperpolarized Silicon Particles

This study develops hyperpolarized silicon particles functionalized with MUC1 antibody for targeted molecular MRI of colorectal cancer, showing active tumor targeting in vivo.

Structural Model of Bacteriophage P22 Scaffolding Protein in a Procapsid by Magic-Angle Spinning NMR

This study determines the structure of bacteriophage P22 scaffolding protein within its procapsid using magic-angle spinning NMR, revealing a trimer of dimers with both helical and disordered regions.

Long-lived coherences for magnetic interactions in proteins

This paper demonstrates long-lived coherences (LLCs) in proteins, enabling stereospecific detection of magnetic interactions through nuclear spin transitions in glycine residues.

Workflow for Protein N-terminal Acetylation and C-terminal Amidation Application using CcpNmr Analysis v2.4 Platform and Aria2.3 Structure Calculation Software

The paper presents a workflow combining CCPNMR2.4 and ARIA2.3 to handle N-terminal acetylation and C-terminal amidation in protein structure calculation, showing no significant difference from existing methods.