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This study compares the efficiency of RNase HII from Bacillus subtilis and Escherichia coli in processing various ribonucleotides, finding B. subtilis is inefficient at repairing damaged rNMPs.

The paper investigates the structural changes in an engineered feruloyl esterase that enhances its ability to degrade MHET, revealing hydrogen bonding and peptide bond isomerization.

CleaveNet is an AI pipeline that designs protease substrates with high efficiency and selectivity, validated experimentally for MMP13.

This study shows that kynurenine-CKA targets C151 in Keap1 to increase Nrf2 activity and anti-inflammatory effects in macrophages.

The paper introduces Delta PSA, a metric for predicting macrocyclic peptide permeability using MD simulations, achieving lower MSE than existing methods.

This study identifies FNBP4 as a nuclear actin-binding protein that inhibits formin FMN1, revealing its dual role in regulating nuclear actin dynamics.

TubercuProbe prioritizes compound-protein interactions in Mycobacterium tuberculosis using a cross-attention graph-sequence model trained on human chemoproteomic data.

This study shows that frataxin deficiency causes tissue-specific iron homeostasis changes in FXNI151F mice, with brain iron overload and cardiac iron deficiency responses.

This paper identifies how a human AGO-miRNA-trigger complex binds ZSWIM8 for CUL3-mediated polyubiquitylation, leading to target-directed miRNA degradation.

This study determines the structure of P-Rex2 and identifies unique regulatory features compared to P-Rex1, revealing differences in autoinhibition and dynamics.

This study identifies AH001 as a potent TRPV4 inhibitor, revealing its mechanism and demonstrating its analgesic effects in pain models.

This study shows glucose directly binds to glucokinase (GK) to trigger GLP-1 secretion in intestinal L cells, independent of ATP levels, and identifies GK and KATP as key players in this process.

The paper identifies tissue-specific readers and antireaders of symmetric and asymmetric hmC modifications in DNA using proteomics and promoter probe assays in human and mouse cells.

This paper designs soluble, miniaturized MHC proteins that enable stable expression in E. coli and retain peptide and TCR binding specificity.

This study identifies POGZ as a novel component of PRC1.6, showing it represses BMP signaling during neuronal differentiation and links to ASD.

The paper identifies signal attrition in XL-MS as the main cause of poor interactome sampling, not low crosslink yield, and highlights issues with signal splitting and database search tools.

This paper designs de novo protein nanoparticles with integrated functional motifs, demonstrating their ability to form structured assemblies and elicit immune responses in mice.

This paper introduces photoacoustic fingerprinting (PAF), a method that improves molecular imaging by reframing spectral unmixing as a fingerprint recognition problem, achieving better accuracy in challenging conditions.

This paper introduces covalent macrocyclic activity-based probes (cmABPs) that selectively target non-catalytic residues in serine proteases, demonstrating their effectiveness with plasma kallikrein and urokinase plasminogen activator.

This study modifies arylmalonate decarboxylase to expand its substrate scope for synthesizing (R)-enantiomers of sterically hindered carboxylic acids with high enantiopurity.