Neil P. King

This paper designs protein assemblies that switch between structural states using engineered interfaces, demonstrating temperature-responsive transitions from capsids to 2D arrays.

Chaperone, a DataFrame-based framework and agent harness for protein design tools, cut tokens 86% and GPU-hours 64% while raising pass rate from 6% to 12%.

This paper develops a computational method to design glycoproteins with controlled N-linked glycan composition by encoding glycan location and structure into amino acid sequences.

This study identifies two antibody classes that neutralize pneumovirus by targeting a conserved epitope, revealing distinct mechanisms of action through structural and functional analyses.

This study shows that lymphatic networks create spatially distinct environments in draining lymph nodes that influence B cell differentiation, affinity selection, and antibody isotype, which can be manipulated through vaccine design.

The paper shows that the repetitive structure of the Plasmodium CSP protein affects B cell memory, leading to reduced humoral immunity over time.

The paper examines how RTS,S/AS01 vaccine induces transient protective B cell responses against malaria, which are later replaced by non-protective memory B cells, explaining its limited long-term efficacy.

The paper identifies molecular interactions between influenza HAs and MHC-II, showing avian MHC-II enables more efficient entry than human MHC-II for most H5 HAs and reveals structural and functional similarities across different HA subtypes.

This paper improves the immunogenicity of E. coli FimH by displaying it on I53-50 nanoparticles, achieving comparable antibody responses to higher doses of monomeric FimH with reduced adjuvant needs.

This study shows that sustained delivery of a SARS-CoV-2 subunit vaccine in an adjuvanted hydrogel depot improves antibody responses in nonhuman primates compared to bolus vaccination.

This study demonstrates that Staphylococcus epidermidis can be engineered to elicit potent immune responses when applied topically or intranasally, with nasal colonization inducing stronger systemic and mucosal immunity.

The paper redesigns TALE proteins to assemble linear protein-DNA complexes on double-stranded DNA templates, enabling controlled length and application in antigen display.

This study shows that nanoparticle vaccines with oligomannose glycans boost B cell responses and protection against malaria sporozoites compared to other glycan types.

This paper designs synthetic fusogens using machine learning, showing they enable efficient cell-cell and intracellular membrane fusion with higher activity than native SNARE complexes.

This paper designs de novo protein nanoparticles with integrated functional motifs, demonstrating their ability to form structured assemblies and elicit immune responses in mice.

This paper designs self-assembling protein nanoparticle vaccines using AlphaFold2 predictions, demonstrating atomic-level accuracy and potent immune responses in mice.

This paper develops an mRNA-launched nanoparticle vaccine that combines protein nanoparticle and mRNA vaccine advantages, showing enhanced antibody and T cell responses in mice.

This paper describes a designed IL-21 mimic, 21h10, which shows potent antitumor activity in multiple models and reduces toxicity through TNF blockade.

This study identifies human antibodies that broadly inhibit influenza neuraminidase by mimicking its substrate through structural convergence and water-mediated interactions.

This paper presents a computational strategy to design bifaceted protein nanomaterials with two distinct sides, enabling anisotropic functionalization and controlled structural variation.

Stabilizing H5 HA mutations enhance neutralizing antibody responses by shifting epitope targeting toward the receptor binding site in vaccinated mice.

This study shows RSV F evolution can escape some monoclonal antibodies but does not strongly reduce neutralization by human polyclonal sera, and introduces a tool to measure neutralization against RSV strains.

This study uses deep mutational scanning to identify functional and antigenic effects of mutations in rabies glycoprotein, revealing escape mutations for antibodies and constrained regions for vaccine development.

This paper designs multivalent RBD nanoparticle vaccines that overcome immune imprinting and provide broad protection against SARS-CoV-2 variants and sarbecoviruses.

This paper introduces WRAPs, designed proteins that solubilize membrane proteins without detergents, enabling structural studies and vaccine development.

This study designs protein minibinders that act as agonists for TLR3, showing nanomolar binding and biological activity in cell lines.

This study develops a multivalent nanoparticle vaccine against EBOV and SUDV, showing protection in rodents from lethal infection.

This paper identifies an unknown contaminant in protein samples using Cryo-EM and AI tools, leading to improved purification protocols.

This study develops MERS-CoV vaccines using a protein nanoparticle platform, showing they induce strong neutralizing antibodies and protect mice against MERS-CoV challenge.

This study uses deep mutational scanning to analyze H5 hemagglutinin mutations and their impact on pandemic risk factors in influenza viruses.