Discover, rate, and discuss biology preprints.

The paper shows that stochastic resonance enhances detection of low-level peptide signals in single-cell proteomics using TMT and a carrier proteome.

The paper develops pyrazolopyrimidine-based macrocyclic kinase inhibitors targeting AAK1, showing selectivity over related NAK family members and antiviral activity against RNA viruses.

The paper investigates discrepancies between input food composition and recovered protein signals in experimental foodcrusts, highlighting biases in residue analysis.

This study identifies and characterizes a novel exo-(N-)glycanase activity of NGLY1 on N-GlcNAc proteins in vitro, expanding its functional repertoire and linking it to clinical features of NGLY1-CDDG.

This paper introduces a Bayesian nonparametric framework for analyzing single-molecule fluorescence data, demonstrating its effectiveness through synthetic and experimental data.

The paper identifies a small molecule, BRD1732, that is ubiquitinated in cells, leading to proteasome inhibition through accumulation of ubiquitin chains.

This study uses an agentic LLM to design and evaluate 1,002 broad-spectrum antibiotic candidates targeting Enterobacteriaceae, with 774 passing ADMET benchmarks and 391 showing moderate binding to dual targets.

The paper introduces a functional assay to identify non-inhibitory enzyme ligands from bead-displayed compound libraries, applied to E3 ubiquitin ligases, and discovers a novel ligand for VHL.

The paper generalizes Michaelis-Menten theory to account for substrate heterogeneity, showing that apparent parameters depend on mean and variance of energy distributions.

This paper introduces a fluorescent assay to quantify ubiquitination kinetics in targeted protein degradation, enabling measurement of degrader and ligase affinities and ternary complex activity.

This study evaluates the effect of methanol stem-bark extract of Erythrina senegalensis on serum albumin levels in carbon tetrachloride-induced liver damage in rats, finding that it restores serum albumin and reduces liver necrosis.

The paper determines cryo-EM structures of E. histolytica ribosomes, revealing unique features like an RNA triple helix and co-evolved rRNA expansion segments.

The paper evaluates cofactor recycling and permeability in bacterial microcompartments using an in vitro assay and kinetic model of the Pdu system, showing benefits for pathway flux.

This study investigates phospho-regulation and flanking regions in autophagy-related SLiMs using optineurin-LC3B as a case, revealing structural mechanisms and disease mutation impacts.

This study develops a quantitative assay to show that Msp1 recognizes substrates via hydrophobic mismatch between the TMD and lipid bilayer.

The paper uses cryo-EM to analyze membrane proteins, revealing unexpected high-resolution structures of ArnA and AcrB instead of the intended FtsH and YidC complex.

This study identifies suitable tRNA sources for cell-free protein synthesis by evaluating tRNA pools from different E. coli strains and V. natriegens, finding that A19 tRNAs are most effective for in vitro translation.

Proteomics and ubiquitinomics screening of 100 CRBN ligands in two cell lines validated 50 novel neosubstrates and yielded selective phenyl glutarimide degraders of KDM4B, G3BP2 and VCL.

This study identifies TTLL11 as a polyglutamylase that extends primary tubulin chains, revealing a new mechanism in the tubulin code and its interaction with other tubulin modifications.

This study investigates how RNase A and RNase 1 cleave pseudouridine and its derivatives, finding that UpA is cleaved more efficiently than {Psi}pA or m1{Psi}pA.