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This study examines how galactose and manganese supplementation affects Trastuzumab N-glycosylation over time in fed-batch and perfusion bioreactors, showing temporal changes in glycoform composition.

The paper develops a high-throughput synthesis-to-screening method to identify selective USP18 inhibitors from a cyanimide library.

This study shows that BEST1, a chloride channel, is directly activated by extracellular GABA, with structural and functional evidence revealing the mechanism of GABA-induced channel opening.

The paper identifies structural factors affecting CRISPR-Cas9 trans-cleavage activity, including R-loop conformation, spacer length, and HNH domain activity.

This study identifies UPF2's RNA-binding and unfolding activities, showing its role in stabilizing single-stranded RNA and supporting NMD factor interactions.

This study identifies that left-handed glycyl conformations protect against protein aggregation and enhance stability by disrupting beta-strand pairing.

The paper identifies epigenetic marks specifying human DNA replication origins through oxidation of 5-methyl-deoxycytidine by TET enzymes.

The paper shows RAP1 inhibits DNA-PK's end joining function at telomeres by forming a complex with TRF2, preventing telomere fusions.

The study shows the Y951N mutation in POLgamma impairs its ability to switch between DNA synthesis and degradation, causing mtDNA depletion without directly affecting polymerase or exonuclease activity.

This study examines how SARS-CoV-2 RNA-dependent RNA polymerase replicates past an oxidative RNA lesion, finding that it preferentially incorporates rCMP and has higher bypass efficiency for rA:8-oxo-rG than rC:8-oxo-rG.

This study shows ATP and amphiphilic molecules modulate FET protein cluster size in a concentration-dependent manner through non-specific interactions.

This study characterizes the molecular structure and enzymatic mechanism of GLT25D1/COLGALT, identifying its catalytic site and dimeric assembly as critical for collagen lysine modification.

This paper designs an enhanced interferon-lambda variant with improved stability and antiviral activity for nasal drug delivery against respiratory viruses.

The paper identifies a new evolutionarily conserved short linear F-actin binding motif and develops a computational pipeline to identify its containing proteins.

This study investigates the catalytic mechanism of methylisocitrate lyase in Coxiella burnetii, identifying key residues and structural changes involved in its function.

The paper identifies missense mutations that create nuclear export signals, leading to disease, and shows therapeutic strategies can restore function.

The paper examines the receptor-binding specificity of a bovine HPAI H5N1 virus using multiple assays and finds it preferentially binds avian-type receptors.

The paper investigates the role of the [4Fe-4S] cofactor in MUTYH DNA repair by analyzing cancer variants and solving the enzyme's crystal structure.

This paper develops a lipid screening platform to identify efficient and tunable ionizable lipids for mRNA delivery to the lungs, resulting in a lipid that enhances protein expression and shows selective lung targeting.

This study reveals the activation mechanism of human mitochondrial ClpP protease through structures showing how inhibitors induce an allosteric transition to the active state, with implications for cancer therapy.