B. Macek

Toward industrial C8 production: Oxygen intrusion drives renewable n caprylate production from ethanol and acetate via intermediate metabolite production

This study identifies oxygen's role in enabling renewable n-caprylate production from ethanol and acetate via intermediate metabolite synthesis in bioreactors.

Mechanical Strain Activates Planar Cell Polarity Signaling to Coordinate Vascular Cell Dynamics

This study identifies Vangl1 as a key mediator of flow-dependent cell dynamics in vascular remodeling through planar cell polarity signaling.

The T4bSS of Legionella features a two-step secretion pathway with an inner membrane intermediate for secretion of transmembrane effectors

This study investigates the secretion mechanism of transmembrane effector proteins by Legionella T4bSS, revealing a two-step process involving an inner membrane intermediate.

Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1

This study develops nanobodies to monitor Drp1, a mitochondrial fission factor, using proteomics, microscopy, and live-cell imaging techniques to study its dynamics in disease models.

ER-anchored protein sorting controls the fate of two proteasome activators for intracellular organelle communication during proteotoxic stress

The paper identifies ERAPS as a system controlling proteasome activator fate during proteotoxic stress, revealing a trade-off between proteasome function and energy metabolism.

Diurnal rhythm causes metabolic crises in the cyanobacterial mutants of c-di-AMP signalling cascade

The paper investigates the role of c-di-AMP and SbtB in cyanobacterial diurnal metabolism, finding that their disruption leads to metabolic dysregulation and diurnal lethality.

The Arabidopsis U1 snRNP regulates mRNA 3'-end processing

This study identifies U1 snRNP-associated proteins in Arabidopsis and shows their role in regulating mRNA 3'-end processing through premature cleavage and alternative polyadenylation site selection.

Nitrite secretion by cyanobacteria is controlled by the small protein NirP1

This study identifies NirP1 as a regulator of nitrite secretion in cyanobacteria under carbon limitation, revealing its role in coordinating carbon and nitrogen metabolism.

Horizontal transfer of bacteriocin biosynthesis genes requires metabolic adaptation to improve compound production and cellular fitness

This study shows that transferring bacteriocin biosynthesis genes between Staphylococcus aureus strains requires metabolic adaptation to enhance compound production and cellular fitness.

Proteins released into the plant apoplast by the obligate parasitic protist Albugo selectively repress phyllosphere-associated bacteria

The paper identifies three Albugo proteins that selectively inhibit gram-positive bacteria in the phyllosphere, suggesting their potential as biocontrol agents.

A network of cytosolic (co)chaperones promotes the biogenesis of mitochondrial signal-anchored outer membrane proteins

This study identifies cytosolic (co)chaperones that interact with signal-anchored mitochondrial proteins and facilitate their biogenesis.

A nanobody-based toolset to monitor and modify the mitochondrial GTPase Miro1

This study develops Miro1-specific nanobodies as tools for monitoring and modifying Miro1 function in cells, with applications in imaging and targeted degradation.

The Arabidopsis SAC9 Enzyme is enriched in a cortical population of early endosomes and restricts PI(4,5)P2 at the Plasma Membrane

The paper shows SAC9 restricts PI(4,5)P2 at the plasma membrane, preventing its ectopic accumulation in intracellular compartments and affecting clathrin-mediated endocytosis and SH3P2 localization.

Two circadian oscillators in one cyanobacterium

The paper identifies a new KaiA homolog, KaiA3, in Synechocystis sp. PCC 6803, which forms a second circadian oscillator alongside the standard KaiB3-KaiC3 system.

A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component

This study shows that the bacterial effector XopL suppresses plant autophagy by degrading SH3P2, revealing a novel pathogen strategy to evade host defense.

Discovery of a novel small protein factor involved in the coordinated degradation of phycobilisomes in cyanobacteria

The paper identifies NblD, a small protein essential for phycobilisome degradation in cyanobacteria during nitrogen starvation, showing it interacts with β-phycocyanin and is required for coordinated dismantling.

Analysis of Amino Acid Variants in Malignant Melanoma Cells Resistant to BRAF inhibition

The paper uses proteogenomics to analyze amino acid variants in BRAF inhibitor-resistant melanoma cells, identifying PTM changes and predicting effective therapies.

An Integrated Workflow for Enhanced Taxonomic and Functional Coverage of the Mouse Faecal Metaproteome

The paper evaluates sample preparation and analysis strategies for mouse faecal metaproteomics, showing that protocol changes affect results and that Unipept improves taxonomic annotation.

The Novel PII-Interacting Regulator PirC (Sll0944) Identifies 3-Phosphoglycerate Mutase (PGAM) as Central Control Point of Carbon Storage Metabolism in Cyanobacteria

This study identifies PirC as a PII-interacting regulator that inhibits PGAM, controlling carbon flux towards glycogen or polyhydroxybutyrate in cyanobacteria under nitrogen limitation.

Proteome dynamics during antibiotic persistence and resuscitation

This study uses stable isotope labeling to track proteome dynamics during antibiotic persistence and resuscitation in E. coli, revealing widespread protein synthesis and key roles for ribosome-associated proteins.

Analysis of a photosynthetic cyanobacterium rich in internal membrane systems via gradient profiling by sequencing (Grad-seq)

The paper uses Grad-seq to analyze the complexome of Synechocystis 6803, identifying potential RNA chaperones and novel RNA-protein complexes.

An interaction network of RNA-binding proteins involved in Drosophila oogenesis

The paper identifies 26 new RNA-binding protein interactions in Drosophila oogenesis using immunoprecipitation and mass spectrometry, linking nuclear and cytoplasmic mRNA regulation.

The BIR2/BIR3-interacting Phospholipase D gamma 1 negatively regulates immunity in Arabidopsis

The study identifies PLDγ1 as a negative regulator of plant immunity that interacts with BIR2 and BIR3 receptor-like kinases.

beta-actin mRNA interactome mapping by proximity biotinylation

This paper introduces RNA-BioID, a method to map the interactome of endogenous mRNAs, and identifies new RNA binding proteins interacting with β-actin mRNA.

Chlorosis as a developmental program in cyanobacteria: the proteomic fundament for survival and awakening

The paper uses proteomics to study the transition of cyanobacteria from a dormant chlorotic state to vegetative growth, identifying key protein modifications and their roles in survival.