Author

H. W. Virgin

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 8 Immunology· 7 Biochemistry· 1 Pharmacology & Toxicology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 16 Research Focus:host-pathogen & immune response· 15 Organism:human· 9 Organism:mouse· 7 Assay:proteomics· 5 Research Focus:drug response / drug discovery· 4 Research Focus:cell type annotation· 3 Research Focus:cell-cell communication· 1

Frequent Co-Authors

D. Corti· 10 shared A. Telenti· 8 shared E. Cameroni· 8 shared J. E. Bowen· 8 shared A. C. Walls· 7 shared D. Pinto· 7 shared D. Veesler· 7 shared A. De Marco· 6 shared

Activity by Year

2018-2022 · peak 9/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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10 shared papersImmunology

D. Corti

Mutual co-authors in cluster

Author profile →

17 papers

Recent·Most cited

Autophagy protects against high-dose Mycobacterium tuberculosis infection

S. Feng, E. M. Nehls, R. L. Kinsella, S. M. Chavez, S. K. Naik, S. R. McKee, N. Dubey, A. Samuels, A. Swain, X. Cui, S. V. Hendrix, R. Woodson, D. Kreamalmeyer, A. Smirnov, M. N. Artyomov, , C. L. Stallings

This study shows autophagy in myeloid cells protects against high-dose Mycobacterium tuberculosis infection by limiting myeloid-derived suppressor cells and promoting T cell responses, distinct from its role in low-dose infection.

bioRxiv · Nov 4, 2022Microbiology

Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape

R. Marzi, J. Bassi, C. Silacci-Fregni, I. Bartha, F. Muoio, K. Culap, N. Sprugasci, G. Lombardo, C. Saliba, E. Cameroni, A. Cassotta, J. S. Low, A. C. Walls, M. McCallum, M. A. Tortorici, , L. Piccoli

The paper examines the kinetics, avidity, and cross-reactivity of SARS-CoV-2-specific memory B cells and antibodies in infected and vaccinated individuals over 16 months, finding that MBC maturation enhances resilience to viral escape.

bioRxiv · Sep 30, 2022Immunology

Robust and durable prophylactic protection conferred by RNA interference in preclinical models of SARS-CoV-2

Y. I. Anglero-Rodriguez, F. A. Lempp, J. McIninch, M. K. Schlegel, C. R. Brown, D. J. Foster, A. B. Castoreno, T. Nguyen, M. Subramanian, M. Montiel-Ruiz, H. Kaiser, A. Sahakyan, R. Spreafico, S. S. Morskaya, J. D. Barry, , V. Jadhav

This study identifies two siRNA combinations that provide robust prophylactic protection against SARS-CoV-2 in hamsters by reducing viral load and preventing lung pathology.

bioRxiv · Mar 20, 2022 · PreprintPharmacology & Toxicology

Resilience of S309 and AZD7442 monoclonal antibody treatments against infection by SARS-CoV-2 Omicron lineage strains

J. B. Case, S. Mackin, J. Errico, Z. Chong, E. A. Madden, B. Guarino, M. A. Schmid, K. Rosenthal, K. Ren, A. Jung, L. Droit, S. A. Handley, P. J. Halfmann, Y. Kawaoka, J. E. Crowe, , M. S. Diamond

This study evaluates the effectiveness of S309 and AZD7442 monoclonal antibodies against Omicron variants in mice, showing reduced lung infection despite decreased neutralization in vitro.

bioRxiv · Mar 17, 2022Microbiology

Structural basis of SARS-CoV-2 Omicron immune evasion and receptor engagement

M. McCallum, N. Czudnochowski, L. E. Rosen, S. K. Zepeda, J. E. Bowen, J. R. Dillen, A. Powell, T. Croll, J. Nix, H. W. Virgin, D. Corti, G. Snell, D. Veesler

This study determines the structural basis of SARS-CoV-2 Omicron's immune evasion and receptor engagement through cryo-EM and X-ray crystal structures of the spike and RBD bound to antibodies and ACE2.

bioRxiv · Dec 28, 2021Biochemistry

Antibody-mediated broad sarbecovirus neutralization through ACE2 molecular mimicry

Y.-J. Park, A. De Marco, T. N. Starr, Z. Liu, D. Pinto, A. C. Walls, F. Zatta, S. K. Zepeda, J. E. Bowen, K. S. Sprouse, A. Joshi, M. Giurdanella, B. Guarino, J. Noack, R. Abdelnabi, , D. Veesler

This study identifies a broadly neutralizing antibody against sarbecoviruses that targets the ACE2 binding site and shows high resistance to escape mutations.

bioRxiv · Oct 13, 2021Immunology

Autophagy gene-dependent intracellular immunity triggered by interferon-γ

M. R. McAllaster, J. Bhushan, D. R. Balce, A. Orvedahl, A. Park, S. Hwang, M. E. Sullender, D. Sibley, H. W. Virgin

This paper identifies new ATG genes and pathways involved in IFN-γ-induced intracellular immunity against norovirus and Toxoplasma gondii.

bioRxiv · May 10, 2021Microbiology

Structural basis for broad sarbecovirus neutralization by a human monoclonal antibody

M. A. Tortorici, N. Czudnochowski, T. N. Starr, R. Marzi, A. C. Walls, F. Zatta, J. E. Bowen, S. Jaconi, J. di Iulio, Z. Wang, A. De Marco, S. Zepeda, D. Pinto, Z. Liu, M. Beltramello, , J. Bloom

This paper identifies a human monoclonal antibody that broadly neutralizes SARS-CoV-2 variants and zoonotic sarbecoviruses by targeting a conserved epitope on the spike protein.

bioRxiv · Apr 7, 2021 · PreprintImmunology

Antibodies to the SARS-CoV-2 receptor-binding domain that maximize breadth and resistance to viral escape

T. N. Starr, N. Czudnochowski, F. Zatta, Y.-J. Park, Z. Liu, A. Addetia, D. Pinto, M. Beltramello, P. Hernandez, A. J. Greaney, R. Marzi, W. G. Glass, I. Zhang, A. S. Dingens, J. E. Bowen, , Veesler

This study identifies antibodies against SARS-CoV-2 that balance neutralization potency and breadth, with some showing high resistance to viral escape.

bioRxiv · Apr 6, 2021 · PreprintMicrobiology

Membrane lectins enhance SARS-CoV-2 infection and influence the neutralizing activity of different classes of antibodies

F. A. Lempp, L. Soriaga, M. Montiel-Ruiz, F. Benigni, J. Noack, Y.-J. Park, S. Bianchi, A. C. Walls, J. E. Bowen, J. Zhou, H. Kaiser, M. Agostini, M. Meury, E. Dellota, S. Jaconi, , D. Corti

This study identifies membrane lectins that enhance SARS-CoV-2 infection and show how different antibodies neutralize infection through distinct mechanisms.

bioRxiv · Apr 3, 2021 · PreprintMicrobiology

SARS-CoV-2 immune evasion by variant B.1.427/B.1.429

M. McCallum, J. Bassi, A. De Marco, A. Chen, A. C. Walls, J. Di Iulio, M. A. Tortorici, M.-J. Navarro, C. Silacci-Fregni, C. Saliba, M. Agostini, D. Pinto, K. Culap, S. Bianchi, S. Jaconi, , D. Veesler

This study evaluates how the B.1.427/B.1.429 SARS-CoV-2 variant evades neutralizing antibodies through mutations in the RBD and NTD, showing reduced neutralization by both vaccine-induced and infection-derived antibodies.

bioRxiv · Mar 31, 2021 · PreprintImmunology

The dual function monoclonal antibodies VIR-7831 and VIR-7832 demonstrate potent in vitro and in vivo activity against SARS-CoV-2

A. L. Cathcart, C. Havenar-Daughton, F. A. Lempp, D. Ma, M. Schmid, M. L. Agostini, B. Guarino, J. Di iulio, L. Rosen, H. Tucker, J. Dillen, S. Subramanian, B. Sloan, S. Bianchi, J. Wojcechowskyj, , C. M. Hebner

This paper evaluates the in vitro and in vivo efficacy of dual-action monoclonal antibodies VIR-7831 and VIR-7832 against SARS-CoV-2, showing potent neutralization and reduced viral load in a hamster model.

bioRxiv · Mar 9, 2021 · PreprintImmunology

N-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2

M. McCallum, A. De Marco, F. Lempp, M. A. Tortorici, D. Pinto, A. C. Walls, M. Beltramello, A. Chen, Z. Liu, F. Zatta, S. Zepeda, J. di Iulio, J. E. Bowen, M. Montiel-Ruiz, J. Zhou, , D. Veesler

The paper identifies a conserved antigenic supersite on the SARS-CoV-2 N-terminal domain recognized by neutralizing antibodies and shows its vulnerability to mutations in variants.

bioRxiv · Jan 14, 2021Immunology

Neutralizing antibody and soluble ACE2 inhibition of a replication-competent VSV-SARS-CoV-2 and a clinical isolate of SARS-CoV-2.

J. B. Case, P. W. Rothlauf, R. E. Chen, Z. Liu, H. Zhao, A. S. Kim, L.-M. Bloyet, Q. Zeng, S. Tahan, L. Droit, M. X. G. Ilagan, M. A. Tartell, G. K. Amarasinghe, J. P. Henderson, S. Miersch, , S. P. J. Whelan

This study develops two neutralization assays for SARS-CoV-2 using VSV and a clinical isolate, showing high concordance between monoclonal, polyclonal antibodies, and ACE2-Fc in neutralizing activity.

bioRxiv · May 18, 2020Microbiology

Structural and functional analysis of a potent sarbecovirus neutralizing antibody

D. Pinto, Y.-J. Park, M. Beltramello, A. C. Walls, M. A. Tortorici, S. Bianchi, S. Jaconi, K. Culap, F. Zatta, A. De Marco, A. Peter, B. Guarino, R. Spreafico, E. Cameroni, J. B. Case, , D. Corti

This study identifies and characterizes a potent neutralizing antibody, S309, against SARS-CoV-2 and related sarbecoviruses, showing its efficacy in preventing viral entry and potential for therapeutic use.

bioRxiv · Apr 7, 2020Immunology

Identification of anti-norovirus genes in mouse and human cells using genome-wide CRISPR activation screening

R. Orchard, M. E. Sullender, B. F. Dunlap, D. R. Balce, J. G. Doench, H. W. Virgin

This study identifies 57 host genes that inhibit murine norovirus replication in mouse or human cells using CRISPR activation screening, revealing novel antiviral mechanisms.

bioRxiv · Jun 18, 2018Microbiology

High throughput screen identifies IFN-γ-dependent inhibitors of Toxoplasma gondii growth

J. B. Radke, K. L. Carey, S. Shaw, S. R. Metkar, C. Mulrooney, J. P. Gale, J. A. Bittker, R. Hilgraf, E. Comer, S. L. Schreiber, H. W. Virgin, J. R. Perez, L. D. D. Sibley

This study identifies IFN-γ-dependent inhibitors of Toxoplasma gondii growth through a high-throughput screen and characterizes their mechanisms of action.

bioRxiv · Jun 2, 2018Microbiology