J. E. Corn

SP110 sequestration of SP100 protects against toxic filaments during innate immune signaling

The paper identifies SP110 as a negative regulator of type 1 interferon-driven cell death by counteracting SP100's toxic filament formation through CARD domain interactions.

Epitope Engineered Human Haematopoietic Stem Cells are Shielded from CD123-targeted Immunotherapy

This paper shows that epitope-engineered human hematopoietic stem cells are protected from CD123-targeted immunotherapy while maintaining functionality, offering a strategy for tumor-selective therapy.

Comprehensive Interrogation of Synthetic Relationships in the Human DNA Damage Response

The paper maps genetic interactions of DDR genes using CRISPRi screening, identifies key interactions with therapeutic potential, and provides insights into genome maintenance mechanisms.

C-terminal amides mark proteins for degradation via SCF/FBXO31

The paper identifies C-terminal amides as signals for protein degradation via SCF/FBXO31, showing that oxidative stress generates these marks and a mutation disrupts this process, leading to toxicity.

BCAT1 inhibition affects CD8+ T cell activation, exhaustion, and tumoral immunity by altering iron homeostasis

This study shows BCAT1 inhibition alters iron homeostasis, affecting CD8+ T cell activation, exhaustion, and tumor immunity, with potential as a cancer therapy.

Human ATG3 contains a non-canonical LIR motif crucial for its enzymatic activity in autophagy

The paper identifies a non-canonical LIR motif in ATG3 essential for its enzymatic activity in autophagy, showing its role in LC3 lipidation and thioester formation.

Genome editing to model and reverse a prevalent mutation associated with myeloproliferative neoplasms

This study uses CRISPR-Cas9 to model and reverse the JAK2 V617F mutation in human hematopoietic cells, revealing subtle phenotypes not seen with transgenic overexpression.

A genome-wide screen for ER autophagy highlights key roles of mitochondrial metabolism and ER- resident UFMylation

The paper identifies 200 factors involved in human ER-phagy through CRISPRi screening and reveals mitochondrial metabolism and ER-resident UFMylation as key regulators.

The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells

The paper identifies the CUL5 ubiquitin ligase complex as a mediator of resistance to MCL1 and CDK9 inhibitors in lung cancer cells by regulating pro-apoptotic proteins.

In vitro transcribed guide RNAs trigger an innate immune response via the RIG-I pathway

The paper shows that in vitro transcribed guide RNAs trigger a strong innate immune response via the RIG-I pathway when used in CRISPR-Cas9 RNP editing of primary cells.

Disabling Cas9 by an anti-CRISPR DNA mimic

This study shows that AcrIIA4 inhibits Cas9 by binding to Cas9-sgRNA complexes, reducing off-target edits while allowing on-target gene editing in human cells.

Flexibility and design: conformational heterogeneity along the evolutionary trajectory of a redesigned ubiquitin

This study shows that increasing flexibility in designed proteins can help escape local minima during evolution, using redesigned ubiquitin variants with improved binding affinity to USP7.