N. J. Stonehouse

Mechanism of enterovirus VP0 maturation cleavage based on the structure of a stabilised assembly intermediate.

The paper determines the molecular mechanism of VP0 cleavage in enteroviruses using structural and bioinformatic analysis of a provirion intermediate.

Insights into polyprotein processing and RNA-protein interactions in foot-and-mouth disease virus genome replication.

This study investigates how mutations at the 3B3-3C junction of FMDV affect polyprotein processing and replication, showing that altered processing impacts enzyme function and RNA interaction.

Amino acid substitutions in norovirus VP1 dictate cell tropism via an attachment process dependent on membrane mobility.

This study identifies an amino acid substitution in norovirus VP1 that enhances cellular attachment and alters tropism by affecting receptor recruitment and membrane mobility.

Production of antigenically stable enterovirus A71 virus-like particles in Pichia pastoris as a vaccine candidate.

This study produces antigenically stable EVA71 virus-like particles in Pichia pastoris, showing they retain native antigenic conformation and elicit neutralising antibodies comparable to inactivated virus vaccines.

Processing of the hepatitis E virus polyprotein can be mediated by a cellular protease

This paper investigates hepatitis E virus polyprotein processing and finds that it relies on host proteases rather than viral proteases for replication.

Development of Enterovirus anti-viral agents that target the viral 2C protein

This study develops broad-spectrum anti-viral compounds targeting the conserved EV 2C protein, showing low IC50 values and suggesting a role for 2C in capsid assembly.

Protease-independent production of poliovirus virus-like particles in Pichia pastoris: Implications for efficient vaccine development and insights into capsid assembly.

This study produces protease-independent poliovirus VLPs in Pichia pastoris, showing they maintain native antigenicity and thermostability, suggesting potential for improved vaccine development.

Production and characterisation of stabilised PV-3 virus-like particles using Pichia pastoris

This paper produces and characterizes stabilized PV-3 virus-like particles using Pichia pastoris, showing improved thermostability and antigen ratio compared to wild-type PV-3.

A conserved glutathione binding site in poliovirus is a target for antivirals and vaccine stabilisation

This study identifies a conserved glutathione binding site in poliovirus VLPs that can be targeted by antivirals and used to stabilize vaccines.

VelcroVax: a bolt-on vaccine platform technology improves antibody titres against a viral glycoprotein in mice

VelcroVax is a vaccine platform that enhances antibody titres against a viral glycoprotein in mice by combining a modified VLP with a SUMO-tagged antigen.

Positive strand RNA viruses differ in the constraints they place on the folding of their negative strand

The paper investigates differences in conformational constraints on negative strand RNA folding among positive strand RNA viruses, finding significant variation in ribozyme activity and duplex formation.

Structural insight into Pichia pastoris fatty acid synthase

The paper determines the structure of P. pastoris fatty acid synthase using cryoEM, identifying structural differences from S. cerevisiae FAS and resolving the mobile ACP domain.

Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication

This study identifies higher-order structures of the foot-and-mouth disease virus RdRp and their role in RNA replication through cryo-EM and mutagenesis.

The Chikungunya virus nsP3 macro domain inhibits activation of the NF-κB pathway

This study shows that the CHIKV nsP3 macro domain inhibits NF-κB activation by blocking TNF-mediated signaling, with effects dependent on ADP-ribosyl hydrolase activity.

High-Resolution Cryo-EM Reveals Dynamics in the Murine Norovirus Capsid

This study uses cryo-EM to reveal the dynamic conformational changes in murine norovirus capsids, showing flexibility in protruding domains that impact virion stability and infectivity.