P. Kukura

Characterisation of membrane protein interactions by peptidisc-mediated mass photometry

This study demonstrates the use of peptidiscs with mass photometry to quantify interactions of membrane proteins in detergent-free conditions, showing its effectiveness for studying bacterial membrane complexes and antibody interactions.

Cooperativity and induced oligomerisation control the interaction of SARS- CoV-2 with its cellular receptor and patient-derived antibodies

The paper investigates how multivalent interactions between SARS-CoV-2 spike protein, ACE2 receptor, and patient-derived antibodies influence viral entry and antibody inhibition.

Single-protein optical holography

This paper demonstrates single-protein mass measurement using a dark-field scattering microscope with non-common path geometry, achieving five orders of magnitude higher sensitivity than previous methods.

Myosin-5 varies its steps along the irregular F-actin track

This study reveals that myosin-5 takes variable step lengths along F-actin tracks, adapting to angular disorder in the track structure.

A quantitative description for optical mass measurement of single biomolecules

The paper develops a framework for light-based single molecule detection and quantification by simulating mass photometry experiments using Fourier optics and molecular polarizability models.

Label-free tracking and massmeasurement of single proteins onlipid bilayers

This paper introduces dynamic mass photometry to track and measure single membrane proteins, revealing that dynamin1 forms dimers rather than tetramers during oligomerization.

Single Molecule Mass Photometry Reveals Dynamic Oligomerization of Plant and Human Peroxiredoxins for Functional Conservation and Diversification

This study uses single molecule mass photometry to analyze the dynamic oligomerization of plant and human 2-CysPRXs, revealing conserved and divergent functional roles across species.

Quantifying the monomer-dimer equilibrium of tubulin with mass photometry

This study uses mass photometry to quantify the monomer-dimer equilibrium of tubulin, clarifying its energetics and kinetics.

Single molecule mass photometry of nucleic acids

This paper demonstrates that mass photometry can detect and quantify nucleic acids with high accuracy and speed, using sub-picomole samples.

The linear ubiquitin chain assembly complex generates heterotypic ubiquitin chains

This study shows LUBAC generates heterotypic ubiquitin chains with linear and oxyester-linked branches through coordinated action of HOIP and HOIL-1L.

Mass Photometry of Membrane Proteins

This study uses mass photometry to analyze membrane proteins and mimetic systems, revealing structural insights and improving nanodisc assembly characterization.

Quantifying protein-protein interactions by molecular counting with mass photometry

This paper demonstrates mass photometry's ability to quantify protein-protein interactions by counting molecules and measuring binding affinities without labels.

Visualization of myosin II filament dynamics in remodeling acto-myosin networks with interferometric scattering microscopy

This study uses interferometric scattering microscopy to visualize myosin II filament dynamics in acto-myosin networks, showing how ATP concentration affects dwell times and network remodeling.

Quantifying the heterogeneity of macromolecular machines by mass photometry

This paper demonstrates that mass photometry accurately quantifies sample heterogeneity with high resolution and minimal volume, outperforming negative stain electron microscopy in detecting proteasome assemblies.

Complementary studies of lipid membrane dynamics using iSCAT and super-resolved Fluorescence Correlation Spectroscopy

This paper compares STED-FCS and iSCAT techniques for measuring lipid diffusion on SLBs using hybrid fluorescent-gold nanoparticle tags, finding significant differences in diffusion coefficients.

Label-free visualization of type IV pili dynamics by interferometric scattering microscopy

This study uses interferometric scattering microscopy to visualize type IV pili dynamics in Pseudomonas aeruginosa, revealing crawling behavior and rapid retraction after surface attachment.

Quantitative mass imaging of single molecules in solution

This paper introduces a method to mass-image single biomolecules in solution with high precision and resolution, enabling the study of molecular interactions and assemblies.