C. V. Robinson

Characterisation of membrane protein interactions by peptidisc-mediated mass photometry

This study demonstrates the use of peptidiscs with mass photometry to quantify interactions of membrane proteins in detergent-free conditions, showing its effectiveness for studying bacterial membrane complexes and antibody interactions.

Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar

The paper uses cryo-EM to determine the gas-phase structure of β-galactosidase at 2.6 Å resolution, showing structural similarity to its native state.

Conserved cardiolipin-mitochondrial ADP/ATP carrier interactions assume distinct structural and functional roles that are clinically relevant

This study investigates the role of cardiolipin interactions in the ADP/ATP carrier using yeast Aac2, showing that disrupting these interactions impairs structure and transport activity, with clinical relevance in ANT1 mutations.

Cellular production of a de novo membrane cytochrome

The paper designs and produces a synthetic diheme membrane cytochrome in bacteria that mimics natural electron transport features without natural sequence homology.

Ubiquitin-like cGAS chain formation by a super enzyme activates anti-phage response

This study identifies a bacterial E2 enzyme that regulates cGAS by mimicking the ubiquitination cascade to activate anti-phage response through poly-cGASylation.

Structural basis of DNA packaging by a ring-type ATPase from an archetypal viral system

This study describes the structural and biochemical mechanisms of DNA packaging by the HK97 bacteriophage's terminase complex, revealing similarities to other viral systems.

Multi-marker metabarcoding resolves subtle variations in freshwater condition: Bioindicators, ecological traits, and trophic interactions

This study uses DNA metabarcoding to assess freshwater ecological condition by analyzing taxonomic and trophic diversity across sites with varying habitat quality.

CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7

The paper uses cryoEM to study endogenous V-ATPase interactions with mEAK-7 in porcine kidney, revealing structural insights and functional differences compared to yeast.

Cryo-EM structures of pentameric autoinducer-2 exporter from E. coli reveal its transport mechanism

The paper reports cryo-EM structures of AI-2 exporters from E. coli, revealing their pentameric structure and elevator-type transport mechanism.

Ion currents through Kir potassium channels are gated by anionic lipids.

The paper shows anionic lipids gate potassium conduction through Kir channels by interacting with the internal gate via fatty acyl tails.

Colicin-mediated transport of DNA through the iron transporter FepA

This study shows colicin B uses the iron transporter FepA to transport DNA into E. coli, revealing a mechanism for cargo delivery through bacterial membranes.

Structural basis for silicon uptake by higher plants

This study presents the X-ray structure and molecular dynamics simulations of OsNIP2;1, revealing how silicon is taken up by plant roots through aquaporins.

Acyl Carrier Protein is essential for MukBEF action in Escherichia coli chromosome organization-segregation

The paper shows Acyl Carrier Protein (AcpP) is essential for MukBEF function in Escherichia coli chromosome organization-segregation.

Competitive binding of MatP and topoisomerase IV to the MukB dimerization hinge

The paper shows MatP and topoisomerase IV ParC compete for binding to the MukB dimerization hinge, with matS binding displacing MatP from the hinge.

Architecture of cell-cell junctions in situ reveals a mechanism for bacterial biofilm inhibition

The paper reveals the structure and function of CdrA in Pseudomonas aeruginosa biofilms, showing how it mediates cell-cell interactions and how targeting it can inhibit biofilm formation.

Insights into glycan import by a prominent gut symbiont

This study characterizes the SusCD protein complex in Bacteroides thetaiotaomicron to elucidate its role in glycan uptake, revealing structural and functional insights into ligand binding and transport dynamics.

Allosteric inhibition of the SARS-CoV-2 main protease - insights from mass spectrometry-based assays

The paper uses mass spectrometry to study SARS-CoV-2 main protease dimerization and allosteric inhibition by small molecules.

Correlating glycoforms of DC-SIGN with stability using a combination of enzymatic digestion and ion mobility MS

This study uses mass spectrometry to show that the stability of DC-SIGN is better correlated with the number of glycosylation sites than with glycan mass or length.

Pyocin S5 import into Pseudomonas aeruginosa reveals a generic mode of bacteriocin transport

The paper identifies the import mechanism of Pyocin S5 in Pseudomonas aeruginosa, revealing a generic pathway for TonB-dependent bacteriocins across Gram-negative bacteria.

Spontaneous oligomerization of BAK/BAX is suppressed by hetero-dimerization with MCL-1

This study shows that BAK/BAX spontaneous oligomerization is suppressed by MCL-1 hetero-dimerization, with BH3-only proteins disrupting these interactions to promote apoptosis.

Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF

The paper examines how nucleotide binding and ATP hydrolysis affect the architecture of the Escherichia coli SMC complex MukBEF.

State-Dependent Lipid Interactions with the A2a Receptor Revealed by MD Simulations Using In Vivo-Mimetic Membranes

This study uses MD simulations to show how specific lipids interact with the A2a receptor in different conformational states, revealing their role in regulating receptor function.

Structures of DPAGT1 explain glycosylation disease mechanisms and advance TB antibiotic design

This paper determines the structures of DPAGT1 with substrates and inhibitors, revealing catalytic mechanisms and disease-causing mutations, and designs non-toxic antibiotics for tuberculosis.

Architecture of TAF11/TAF13/TBP complex suggests novel regulatory state in General Transcription Factor TFIID function

The paper identifies a ternary TAF11/TAF13/TBP complex and reveals a novel regulatory state in TFIID function through structural and biochemical analyses.