R. J. Botelho

Control of GSK3beta nuclear localization by amino acid signaling requires GATOR1 but is mTORC1 independent

The paper shows that GATOR1 regulates GSK3β nuclear localization in response to amino acids, independent of mTORC1, and that this process supports cell survival under metabolic stress.

Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent manner

This study shows Salmonella modulates TFEB in macrophages depending on its growth phase and infection time, with stationary-phase Salmonella delaying TFEB nuclear mobilization while late-log Salmonella allows TFEB activation.

The LCLAT1/LYCAT acyltransferase is required for EGF-mediated phosphatidylinositol-3,4,5-trisphosphate generation and Akt signalling

This study shows that LCLAT1 is required for EGF-mediated PtdIns(3,4,5)P3 generation and Akt signaling in cancer and retinal cells.

Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence

This paper demonstrates that silver nanoparticles enhance lysosome fluorescence, enabling live-cell imaging with reduced phototoxicity and preserved dynamics.

AMP-activated protein kinase (AMPK) is required for recovery from metabolic stress induced by ultrasound microbubble treatment

This study investigates how AMPK mediates cellular recovery from metabolic stress caused by ultrasound microbubble treatment in breast cancer cells.

PIKfyve inhibition reveals a novel role for Inpp4b in the regulation of PtdIns(3)P and lysosome dynamics

This study identifies a novel crosstalk between Inpp4b and PIKfyve that regulates lysosome membrane phosphoinositide composition and homeostasis by restricting VPS34-dependent PtdIns(3)P production.

Reactive oxygen species prevent lysosome coalescence during PIKfyve inhibition

This study shows that reactive oxygen species (ROS) prevent lysosome coalescence during PIKfyve inhibition through distinct mechanisms depending on the ROS type.

INPP4B drives lysosome biogenesis to restrict leukemic stem cell differentiation and promote leukemogenesis

This study identifies INPP4B's role in promoting leukemic stem cell (LSC) survival by enhancing lysosome biogenesis, leading to poor AML prognosis and suggesting therapeutic targets.

Phagosome resolution regenerates lysosomes and maintains the degradative capacity in phagocytes

This study shows that phagosome resolution through clathrin-dependent fragmentation regenerates lysosomes and maintains macrophage degradative capacity after multiple rounds of phagocytosis.

Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence

This study shows that PIKfyve inhibition causes lysosome coalescence, leading to fewer enlarged lysosomes, and that TFEB-mediated biosynthesis does not drive this swelling.