S. Saito

The priming phosphorylation of KaiC is activated by the release of its autokinase autoinhibition

This study identifies the autoinhibition mechanism of KaiC priming phosphorylation and shows how KaiA relieves this inhibition by altering R385 interactions.

UGGT1-mediated reglucosylation of N-glycan competes with ER-associated degradation of unstable and misfolded glycoproteins

This study shows UGGT1 reglucosylation competes with EDEM-mediated degradation of misfolded glycoproteins, influencing ER protein quality control.

Indirect CRISPR screening with photoconversion revealed key factors of drug resistance with cell-cell interactions

This study uses indirect CRISPR screening with photoconversion to identify key factors of drug resistance mediated by cell-cell interactions, finding genes that reduce cancer cell apoptosis when knocked out in supporting cells.

Intranasal vaccination induced cross-protective secretory IgA antibodies against SARS-CoV-2 variants with reducing the potential risk of lung eosinophilic immunopathology

This study shows that an intranasal vaccine with CpG ODN2006 induces secretory IgA antibodies against SARS-CoV-2 variants and reduces lung eosinophilic immunopathology compared to subcutaneous vaccination.

Regulation Mechanisms of the Dual ATPase in KaiC

This study investigates how the dual ATPase activities in KaiC coordinate to regulate the cyanobacterial circadian clock through structural and biochemical analysis.

Non-glycosylated Seipin to Cause a Motor Neuron Disease Induces ER stress and Apoptosis by Inactivating the ER Calcium Pump SERCA2b

Ring-shaped multimeric structure enables the acceleration of KaiB-KaiC complex formation induced by the ADP/ATP exchange inhibition

This paper investigates how the ring-shaped hexameric structure of KaiC enables acceleration of KaiB-KaiC complex formation through cooperative binding, despite ADP/ATP exchange inhibition typically slowing processes.

The Mechanism of Neurite Outgrowth Induction by Novel Synthetic Retinobenzoic Acids

This study shows novel synthetic retinobenzoic acids induce neurite outgrowth in human neuroblastoma cells via RARβ and ERK1/2/mTOR pathways, differing from RA's mechanism.

The ATF6β-calreticulin axis protects hippocampal neurons against endoplasmic reticulum stress and excitotoxicity

This study shows that ATF6β-calreticulin protects hippocampal neurons from ER stress and excitotoxicity by maintaining Ca2+ homeostasis.

Antihypertensive effect of brain-targeted mechanical intervention with passive head motion

Passive head motion reduces blood pressure in hypertensive rats and humans by decreasing AT1R expression in RVLM astrocytes through interstitial fluid shear stress.

AMPK regulates cell shape of cardiomyocytes by modulating turnover of microtubules through CLIP-170

This study shows AMPK regulates cardiomyocyte shape by modulating microtubule turnover via CLIP-170 phosphorylation at intercalated discs.

An alternative interpretation of the slow KaiB-KaiC binding of the cyanobacterial clock proteins

This study proposes a new binding mechanism for KaiB-KaiC in cyanobacteria that explains the slowness of the circadian rhythm without requiring conformational changes in KaiB.

Sufficiency of unidirectional allostery in KaiC in generating the cyanobacterial circadian rhythm

This study shows that unidirectional allostery from C2 to C1 in KaiC is sufficient to generate the cyanobacterial circadian rhythm, as demonstrated by matching experimental data on phosphorylation and ATPase activity.