T. Kondo

Enrichment of allelic editing outcomes by Prime Editing in iPS cells

This study compares Cas9 and Prime Editing (PE) in human iPS cells, showing PE's superior precision and demonstrating how sorting by PE expression affects allelic editing outcomes.

Virological characteristics of the SARS-CoV-2 XBB.1.5 variant

The paper analyzes the virological characteristics of the SARS-CoV-2 XBB.1.5 variant, showing reduced pathogenicity and impaired MHC expression due to mutations in ORF8 and S.

Enucleation of the C. elegans embryo revealed the mechanism of dynein-dependent spacing between microtubule asters

The paper uses enucleated C. elegans embryos to study dynein-dependent centrosome spacing mechanisms and identifies a dynein-independent movement in later cell cycles.

Microbiome ssRNA as an environmental cue to activate TLR13-dependent tissue-protective programs in CD5Lhi hepatic macrophages

The paper shows that microbiome ssRNA activates TLR13 in hepatic macrophages to drive tissue-protective responses, leading to resistance against acute liver injury in Rnaset2-/- mice.

Mouse gingival single-cell transcriptomic atlas: An activated fibroblast subpopulation guides oral barrier immunity in periodontitis

This study identifies an activated fibroblast subpopulation in mouse periodontitis that drives oral inflammation via interactions with neutrophils and ILC3s.

Apoptotic contraction drives target cell release by cytotoxic T cells

This study identifies apoptotic contraction as a mechanism driving CTL dissociation and target cell release.

Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generation

This paper develops a method to estimate crossbridge-state in cardiomyocytes using SHG polarization, showing its applicability in assessing myosin force generation and drug effects.

Charge neutralization and β-elimination cleavage mechanism of family 42 L-rhamnose-α-1,4-D-glucuronate lyase revealed using neutron crystallography

This paper uses neutron crystallography to reveal the charge neutralization and β-elimination mechanism of FoRham1, an enzyme that cleaves gum arabic.

Comprehensive analysis of the effect of mRNA sequences on translation efficiency and accuracy

The paper analyzes how mRNA sequences affect translation efficiency and accuracy, identifying key codons and bases that influence ribosome behavior and misreading rates.

Dynamic interaction of BcsD subunit in type I bacterial cellulose synthase

This study investigates the dynamic interaction of BcsD in type I bacterial cellulose synthase, showing its role in regulating cellulose crystallization through two interaction modes.

Lipid-correlated alterations in the transcriptome are enriched in several specific pathways in the postmortem prefrontal cortex of Japanese patients with schizophrenia

The paper integrates transcriptome and lipidome data from postmortem prefrontal cortex samples of schizophrenia patients to identify lipid-correlated gene expression changes in specific pathways.

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

This study identifies a compound, Dwn1, that downregulates Npas2 and accelerates wound healing with reduced scarring in mice.

Regulation Mechanisms of the Dual ATPase in KaiC

This study investigates how the dual ATPase activities in KaiC coordinate to regulate the cyanobacterial circadian clock through structural and biochemical analysis.

Elucidation of Master Allostery Essential for Circadian Clock Oscillation in Cyanobacteria

The paper identifies the structural basis for allostery in the cyanobacterial circadian clock by analyzing KaiC protein states and shows how coupling between a phospho-switch and ATPase activity drives oscillation.

WEE1 kinase is a therapeutic vulnerability in CIC-DUX4 undifferentiated sarcoma.

This study identifies WEE1 as a therapeutic target in CIC-DUX4 sarcomas by showing its dependency on WEE1 activity for survival.

Spatial heterogeneity of glioblastoma cells reveals sensitivity to NAD+ depletion at tumor edge

This study identifies clonal subpopulations in glioblastoma core and edge lesions and shows that NAD+ depletion via CD38 inhibition reduces tumor edge formation.

Brain aging-dependent glioma traits reversible by NAD+/BDNF-mediated neuronal reactivation

This study shows that aging reduces neuronal signaling in gliomas, but NAD+ and BDNF can reverse these effects, improving outcomes in older mice.

Tumor Edge-to-Core Transition Promotes Malignancy in Primary-to-Recurrent Glioblastoma Progression in a PLAGL1/CD109-mediated mechanism

The paper identifies a CD133down/CD109up signature associated with poor prognosis in glioblastoma recurrence and shows PLAGL1 mediates tumor progression through CD109 regulation.

An improved molecular tool for screening bacterial colonies using GFP expression enhanced by a Dictyostelium sequence

The paper introduces a molecular tool using TED-enhanced GFP for efficient screening of bacterial colonies without an inducer.

Localization of BCR-ABL to stress granules contributes to its oncogenic function

This study shows BCR-ABL localizes to stress granules, and this localization contributes to its oncogenic function in CML.

Pivotal involvement of the CX3CL1-CX3CR1 axis for the recruitment of M2-TAMs in skin carcinogenesis

The paper investigates the role of the CX3CL1-CX3CR1 axis in skin carcinogenesis, finding that it promotes tumor-associated macrophage recruitment and function, contributing to cancer progression.

Membrane Protrusion Formation Mediated by Rho/ROCK Signalling and Modulation of Chloride Flux

This study shows Prom1 triggers membrane protrusions via Rho/ROCK signaling and chloride efflux, independent of actin/tubulin polymerization.

Translation enhancement by a Dictyostelium gene sequence in Escherichia coli

The paper introduces a method to enhance protein expression in E. coli using a Dictyostelium gene sequence upstream of the Shine-Dalgarno sequence, showing increased green fluorescent protein production.

CDK1 dependent phosphorylation of hTERT contributes to cancer progression

The paper shows CDK1 phosphorylates hTERT at threonine 249, which is required for RdRP activity and promotes cancer progression independently of telomerase.

The CIC-DUX4 fusion oncoprotein drives metastasis and tumor growth via distinct downstream regulatory programs and therapeutic targets in sarcoma

This study identifies ETV4 and CCNE1 as targets of the CIC-DUX4 fusion oncoprotein, showing their roles in sarcoma metastasis and tumor growth, and highlights therapeutic strategies targeting the CCNE-CDK2 complex.

Impaired chromosome segregation results in sperms with excess centrosomes in emb-27APC6 mutant C. elegans

This study shows that emb-27 mutants in C. elegans have excess centrosomes in sperms, leading to zygotes with excess centrosomes and reduced spermatid numbers.

Choice between 1- and 2-furrow cytokinesis in Caenorhabditis elegans embryos with tripolar spindles

The paper investigates how Caenorhabditis elegans embryos with tripolar spindles form 1 or 2 cytokinetic furrows, finding that cell shape, cortical forces, and centrosome size determine this outcome.