H. Yamaguchi

De novo design of mini-proteins as specific binders to PACAP-38, a key mediator of migraine

This study designs mini-proteins that bind PACAP-38 with high affinity, showing improved potency and stability compared to earlier versions.

An efficient inducible model for the control of gene expression in renin cells

This paper develops an inducible mouse model to specifically control gene expression in renin cells, demonstrating efficient and targeted deletion of renin in adult mice without developmental effects.

Label-free ghost cytometry for manufacturing of cell therapy products

This paper introduces label-free ghost cytometry (LF-GC) for cell therapy manufacturing, showing it can quantify cell count, viability, and identify cell subsets and impurities without staining.

Dose-response relationship for the resistance of human insulin to degradation by insulin-degrading enzyme

The paper investigates how human insulin resistance to degradation by IDE varies with dose and identifies structural features of the insulin hexamer that influence degradation kinetics without zinc.

Calaxin stabilizes the docking of outer arm dyneins onto ciliary doublet microtubule in vertebrates

The paper investigates the role of Calaxin in stabilizing outer arm dynein docking onto ciliary microtubules in vertebrates using zebrafish and structural analysis.

Engineering the substrate specificity of toluene degrading enzyme XylM using biosensor XylS and machine learning

This paper uses a biosensor and machine learning to engineer XylM for improved substrate specificity, achieving 15 times higher productivity than the wild-type.

NF-κB Decoy ODN-Loaded Poly lactic-co-glycolic Acid Nanospheres Inhibit Alveolar Ridge Resorption

This study investigates the therapeutic effect of NF-κB decoy ODN-loaded PLGA nanospheres in reducing alveolar ridge resorption after tooth extraction in rats.

Reconstitution of prenyltransferase activity on nanodiscs by components of the rubber synthesis machinery of the Para rubber tree and guayule

The paper reconstitutes prenyltransferase activity using proteins from Para rubber tree and guayule in nanodiscs, showing that HRT1 and HRBP form an active complex.

A pipeline for rapidly generating genetically engineered mouse models of pancreatic cancer using in vivo CRISPR-Cas9 mediated somatic recombination

This paper develops a CRISPR-Cas9-based pipeline to generate pancreatic cancer mouse models with complex genotypes using AAV-delivered sgRNAs in adult mice.

Evolutionary history of phycoerythrin pigmentation in the water bloom-forming cyanobacterium Microcystis aeruginosa

This study investigates the genomic basis and evolutionary history of phycoerythrin pigmentation in Microcystis aeruginosa, revealing horizontal gene transfer and ecological adaptation as key factors.

Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly

The paper studies zebrafish PIH proteins to determine their roles in axonemal dynein assembly and cilia/flagella motility, finding that each PIH protein is associated with specific dynein subtypes.