A. Antanasijevic

Anti-Immune Complex Antibodies are Elicited During Repeated Immunization with HIV Env Immunogens

The paper identifies anti-immune complex antibodies generated through repeated HIV Env immunizations and presents their structures using polyclonal epitope mapping.

Enterovirus A71 adaptation to heparan sulfate comes with capsid stability tradeoff

This study shows that EV-A71 adapts to heparan sulfate by losing pH-dependent uncoating, which compromises capsid stability and affects viral fitness in vivo.

Influence of amino acid substitutions in capsid proteins of coxsackievirus B5 on free chlorine and thermal inactivation

This study investigates how amino acid substitutions in coxsackievirus B5 capsid proteins affect sensitivity to free chlorine and heat, finding that capsid changes have a minor role in chlorine sensitivity but reduce heat sensitivity in specific mutants.

Local structural flexibility drives oligomorphism in computationally designed protein assemblies

This study shows that structural flexibility in computationally designed protein assemblies leads to multiple architectures, expanding their functional possibilities.

Glycan heterogeneity as a cause of the persistent fraction in HIV-1 neutralization

This study investigates how glycan heterogeneity on HIV-1 Env contributes to persistent infectivity despite neutralizing antibody (NAb) treatment, showing that glycan variants affect antibody binding and neutralization efficacy.

Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies

The paper structures and characterizes Lassa virus glycoproteins from multiple lineages and identifies neutralizing antibodies that target conserved epitopes.

Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains

This study identifies cryptic transmembrane domains in designed protein nanoparticles that hinder secretion and introduces a computational tool, Degreaser, to remove them while maintaining stability, improving secretion efficiency.

Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimers as HIV-1 vaccine candidates

Glycan trimming of HIV-1 Env immunogens improves vaccine-induced neutralizing antibody responses in small animals and primates.

Lassa virus glycoprotein nanoparticles elicit a neutralizing antibody that defines a new site of vulnerability

This study develops Lassa virus glycoprotein nanoparticles that stabilize GPC trimers and elicit neutralizing antibodies targeting a new vulnerability site.

A mRNA vaccine encoding for a RBD 60-mer nanoparticle elicits neutralizing antibodies and protective immunity against the SARS-CoV-2 delta variant in transgenic K18-hACE2 mice

High-resolution structural analysis of enterovirus-reactive polyclonal antibodies in complex with whole virions

This study uses EMPEM to map polyclonal antibodies against NPEV virions, identifying cross-reactive epitopes on the virus surface.

Structural mapping of antibody landscapes to human betacoronavirus spike proteins

The paper maps epitopes of polyclonal antibodies against beta-coronavirus spike proteins in human sera, revealing cross-reactive patterns between seasonal and SARS-2 infections.

From Structure to Sequence: Identification of polyclonal antibody families using cryoEM

This paper uses cryoEM and next-generation sequencing to identify monoclonal antibodies from polyclonal sera and validate their antigen-binding properties.

Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM

The paper uses cryoEM to analyze polyclonal antibody responses to HIV Env immunogens in rhesus macaques, revealing structural differences in antibody binding and immunogenicity of engineered mutations and glycan sites.

Immunofocusing and enhancing autologous Tier-2 HIV-1 neutralization by displaying Env trimers on two-component protein nanoparticles

This study shows that displaying HIV-1 Env trimers on two-component protein nanoparticles enhances autologous Tier-2 neutralizing antibody titers in rabbits.

Structural analysis of full-length SARS-CoV-2 spike protein from an advanced vaccine candidate

The paper uses cryo-EM and glycan analysis to study the structure of a full-length SARS-CoV-2 spike protein in a vaccine candidate, revealing its stable prefusion conformation and novel trimer interactions.

Enhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike

The paper modifies HIV-1 Env trimers to increase glycan occupancy and reduce artificial glycan holes, improving vaccine antigen design.

Targeting HIV Env immunogens to B cell follicles in non-human primates through immune complex or protein nanoparticle formulations

This study shows that HIV Env immunogens can be concentrated in B cell follicles using immune complexes or protein nanoparticles, enhancing their localization in lymph nodes and potentially improving immune responses.

Structural and functional evaluation of de novo-designed, two-component nanoparticle carriers for HIV Env trimer immunogens

The paper evaluates de novo-designed two-component nanoparticles for presenting HIV Env trimers, showing improved antibody responses through multivalent display.

HIV-1 Envelope and MPER antibody structures in lipid assemblies

This study presents methods to incorporate full-length HIV-1 Env into lipid assemblies for structural analysis, revealing the quaternary epitope of an MPER antibody and Env tilting during neutralization.