W. J. Chazin

A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α-primase

This study presents cryo-EM structures of DNA polymerase α-primase complexes to elucidate the mechanism of primer synthesis and its regulation.

Structure of RADX and mechanism for regulation of RAD51 nucleofilaments

This paper investigates the structure and mechanism of RADX, a negative regulator of RAD51 nucleofilaments, showing it caps and restricts RAD51 filament growth to regulate replication fork reversal.

Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase

This study investigates RNA primer synthesis and handoff in human DNA polymerase α-primase, showing that flexibility and distributive synthesis are key to primer generation and transfer.

XPA tumor variants lead to defects in NER that sensitize cells to cisplatin

This study identifies XPA tumor variants that impair NER, increasing cisplatin sensitivity, and provides mechanistic insights for variant effect prediction.

Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection

This study shows that the commensal bacterium Bacteroides thetaiotaomicron acquires iron using siderophores from Salmonella, evading host sequestration by lipocalin-2 and allowing Salmonella to bypass nutritional immunity.

Two Interaction Surfaces between XPA and RPA Organize the Preincision Complex in Nucleotide Excision Repair

The paper investigates two interaction surfaces between XPA and RPA in the preincision complex of nucleotide excision repair, showing their distinct roles in complex assembly and NER activity.

An active learning framework improves tumor variant interpretation

This study applies active learning to improve variant interpretation for tumor genomes, showing better performance than traditional methods in predicting variant effects on cancer treatment.

Neutrophil-associated responses to Vibrio cholerae infection in a natural host model

This study investigates the role of calprotectin and metal limitation in inhibiting Vibrio cholerae growth in zebrafish, showing their potential as therapeutic targets.

The zinc transporter ZnuABC is critical for the virulence of Chromobacterium violaceum and contributes to diverse zinc-dependent physiological processes

Molecular basis and functional consequences of the interaction between the Base Excision Repair DNA glycosylase NEIL1 and RPA

This study identifies two molecular interaction points between NEIL1 and RPA using biophysical methods and shows that disrupting this interaction does not impair NEIL1's activity against oxidative DNA damage in vitro but reveals a role in nucleotide excision repair in vivo.

Identification of ubiquitin Ser57 kinases regulating the oxidative stress response in yeast

This study identifies Ser57 ubiquitin kinases in yeast and human cells that regulate the oxidative stress response by phosphorylating ubiquitin at Ser57.