I. E. Brodsky

Lipid Nanoparticle-Associated Inflammation is Triggered by Sensing of Endosomal Damage: Engineering Endosomal Escape without Side Effects

This paper identifies that lipid nanoparticle-induced inflammation arises from endosomal membrane damage and proposes strategies to mitigate it through galectin inhibition or ESCRT-recruiting lipids.

Sequentially activated death complexes regulate pyroptosis and IL-1β release in response to Yersinia blockade of immune signaling

This study shows that Yersinia-induced cell death and IL-1β release are driven by spatially and temporally distinct but functionally connected death complexes.

Forward genetic screening in engineered colorectal cancer organoids identifies novel regulators of metastasis

The paper uses CRISPR/Cas9 screening in colorectal cancer organoids to identify novel metastasis regulators, finding Ctnna1 and Bcl2l13 as metastasis suppressors.

Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

This study identifies caspase-1 and GSDMD as key mediators of inflammasome responses that restrict Salmonella replication in human macrophages.

Blockade of IKK signaling induces RIPK1-independent apoptosis in human cells

This study shows that IKK blockade induces RIPK1-independent apoptosis in human cells, contrasting with murine models where RIPK1 is required.

A TNF-IL-1 circuit controls Yersinia within intestinal granulomas

This study identifies a monocyte-intrinsic TNF-IL-1 circuit that controls Yersinia infection within intestinal granulomas by signaling to non-hematopoietic cells.

Yersinia Type III-Secreted Effectors Evade the Caspase-4 Inflammasome in Human Cells

This study shows Yersinia pseudotuberculosis effectors evade the caspase-4 inflammasome in human cells, unlike in mouse macrophages, by inhibiting Yersinia internalization.

Noncanonical inflammasome assembly requires caspase-11 catalytic activity and intra-molecular autoprocessing

This study shows that Casp11 catalytic activity and autoprocessing are required for noncanonical inflammasome assembly and LPS-induced speck formation in macrophages.

TNF licenses macrophages to undergo rapid caspase-1, -11, and -8-mediated cell death that restricts Legionella pneumophila infection

This study shows TNF enables macrophages to undergo rapid caspase-mediated cell death, which restricts Legionella pneumophila infection.

Inflammatory monocytes promote pyogranuloma formation to counteract Yersinia blockade of host defense

This study shows that Yersinia pseudotuberculosis induces pyogranulomas in the murine intestine by blocking phagocytosis, and that monocytes are essential for granuloma formation and immune control.

Arresting microbiome development limits immune system maturation and resistance to infection.

This study shows that delaying microbiome development during weaning impairs immune system maturation and increases susceptibility to infection.

Position-specific secondary acylation determines detection of lipid A by murine TLR4 and caspase-11

The paper investigates how position-specific secondary acyl chains on lipid A affect TLR4 and Casp11 activation, revealing distinct structural requirements for each receptor.

Human TLR4 and noncanonical inflammasome differ in their ability to respond to distinct lipid A variants

The paper compares how human TLR4 and noncanonical inflammasome sensors CASP4/5 respond to different lipid A structures, finding that acylation state differentially affects their activation.

Salmonella Typhimurium induces NAIP/NLRC4- and NLRP3/ASC-independent, caspase-1/4-dependent inflammasome activation in human intestinal epithelial cells

This study shows that human intestinal epithelial cells use caspase-1/4-dependent inflammasomes, not NAIP/NLRC4 or NLRP3/ASC, to respond to Salmonella Typhimurium.

Genetic targeting of Card19 is linked to disrupted Ninj1 expression, impaired cell lysis, and increased susceptibility to Yersinia infection

This study shows that genetic targeting of Card19 disrupts Ninj1 expression, impairs macrophage cell lysis, and increases susceptibility to Yersinia infection.