Gavin J. Sherlock

The Candida Genome Database: New Interface and New Tools

The paper describes updates to the Candida Genome Database, including a new interface and tools for improved data access and analysis.

Capsular polysaccharide can sensitize bacteria to non-antibiotic drugs

The paper shows that the K5 capsular polysaccharide of E. coli Nissle 1917 sensitizes it to thioridazine and related antipsychotics, revealing CPS as a vulnerability under non-antibiotic drug pressure.

High-throughput DNA engineering by mating bacteria

This paper introduces SCRIVENER, a bacterial mating-based DNA assembly platform that enables high-throughput, low-error DNA construction of up to 23 kb constructs.

Experimental Evolution of Yeast Reveals Trade-offs Between Early and Late Stationary Phase

This study experimentally evolves yeast to show that mutations beneficial in early stationary phase are detrimental in late stationary phase, indicating a fitness trade-off.

Centromeres evolve progressively through selection at the kinetochore interface

The paper investigates how new centromeres evolve and become fixed in budding yeast by analyzing 138 species and 2,500 isolates, showing that kinetochore interface constraints influence centromere evolution.

Analysis and culturing of the prototypic crAssphage reveals a phage-plasmid lifestyle

This study characterizes the crAssphage lifestyle as a phage-plasmid, demonstrating its persistence in the gut microbiome without causing host lysis or chromosomal integration.

The Candida Genome Database: Annotation and Visualization Updates

The paper describes updates to the Candida Genome Database, focusing on annotation, visualization, and integration of clinical data for research on Candida species.

Saccharomyces Genome Database: Advances in Genome Annotation, Expanded Biochemical Pathways, and Other Key Enhancements

Saccharomyces Genome Database released two S288C annotation updates (R64.4.1, R64.5.1) adding ncRNAs, ORFs and uORFs, overhauled YeastPathways, and expanded search, GFF and web features.

A shift from pleiotropic to modular adaptation revealed by a high-resolution two-step adaptive walk

This study shows that early adaptive mutations in yeast are pleiotropic, while later mutations are modular, focusing on respiration over fermentation.

Arrayed in vivo barcoding for multiplexed sequence verification of plasmid DNA and demultiplexing of pooled libraries

This paper introduces an in vivo plasmid barcoding method for high-throughput sequence verification and demultiplexing of pooled libraries, demonstrating high accuracy and cost-effectiveness.

Updates to the Alliance of Genome Resources Central Infrastructure

The paper updates the Alliance of Genome Resources' central infrastructure, adding tools and enhancing data management and curation capabilities.

No evidence that synonymous mutations in yeast genes are mostly deleterious

The paper reanalyzes data from Shen et al. to argue that most synonymous mutations in yeast genes are not deleterious, challenging their original conclusions about fitness effects.

S. cerevisiae cells can grow without the Pds5 cohesin subunit

This study shows that S. cerevisiae cells can survive without Pds5 if Mcd1 levels are increased and SUMO-PCNA is elevated, revealing Pds5's role in cohesin stability and DNA replication coordination.