Paul W. Tillberg

This paper uses ex utero culture to study mouse embryogenesis from gastrulation to fetal development, revealing metabolically independent programs and environmental influences on metabolic transitions.

The paper develops WARP, a platform integrating neuronal activity and gene expression data in zebrafish brains, revealing functional roles of molecularly defined cell populations and their activity correlations.

SpotDMix improves mRNA spot assignment to cells in spatial transcriptomics by using a mixture model and EM algorithm, outperforming simple methods in accuracy and exclusivity.

The paper introduces WHOLISTIC, a method to image cellular activity across all organs of a vertebrate, revealing body-wide circuits and responses to stimuli.

TEMI maps biomolecule distributions in tissues at single-cell resolution, revealing metabolic heterogeneity in tumors.

This paper introduces a Bayesian method to estimate the number of fluorescent emitters in diffraction-limited spots, showing improved accuracy over existing methods.

This paper introduces cycleHCR technology for high-resolution spatial transcriptomics and protein imaging in thick tissues, demonstrating its application in mouse embryos and brain slices.

The paper introduces morphoFISH, a method combining imaging and spatial transcriptomics to identify new neuronal cell types in the mouse brain.

This paper develops improved glutamate indicators with faster activation kinetics and better synaptic localization for imaging synaptic transmission in neurons.

This study maps cell types and axon projections in the central nucleus of the amygdala using single-cell RNA-sequencing and spatial techniques, revealing distinct molecular and connectivity patterns.

The paper develops DELTA, a method to measure brain-wide synaptic protein turnover at single-synapse resolution, revealing localized plasticity in hippocampal CA1 during learning.

The paper develops a method to rapidly reconstruct neural circuits with single synapse resolution using tissue expansion and lattice light sheet microscopy, validated in Drosophila.

The paper introduces EASI-FISH, a method for spatial gene expression profiling in thick brain sections, and applies it to discover novel subregions in the lateral hypothalamus.

ExSeq enables multiplexed RNA mapping at nanoscale resolution by expanding biological specimens for in situ sequencing.

The paper shows nitric oxide acts as a cotransmitter in dopaminergic neurons to modulate memory dynamics in Drosophila.

BigStitcher reconstructs high-resolution image datasets from cleared and expanded biological samples, compensating optical distortions for single-cell analysis.