Masato Ohtsuka

A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency

The paper shows that miR-195 can rescue B cell development in EBF1-deficient progenitor cells by enabling CD19 expression and recombination processes.

Revisiting the in vivo functions of coilin in mice: Requirement for snRNA modifications and formation of novel condensates in elongating spermatids

This study investigates the role of coilin in mice by generating coilin mutants, revealing its involvement in snRNA modifications and the formation of novel condensates in spermatids.

Akr1b7 functions as a master regulator in ovarian aging

This study identifies Akr1b7 as a key regulator in ovarian aging, showing its role in oocyte maturation and estrous cycle regulation in mice.

Delivering mRNAs to mouse tissues using the SEND system

This study demonstrates the SEND process in vivo using genetically engineered mouse models, showing potential for using SEND-VLPs as a gene therapy tool for delivering mRNAs to tissues.

Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology

The paper re-evaluates the efficiency of a CRISPR-Cas9 method for generating conditional knockout alleles in mice, finding very low success rates and high rates of undesired alleles.

Alopecia areata susceptibility variant identified by MHC risk haplotype sequencing reproduces symptomatic patched hair loss in mice

The paper identifies a susceptibility variant in CCHCR1 associated with alopecia areata and shows its effect in mice with patched hair loss.

Identification of PTBP1 responsible for caspase dependent YRNA cleavage

The paper identifies PTBP1 as responsible for caspase-dependent YRNA cleavage during apoptosis, revealing its role in ASR biogenesis.

In situ genome editing method suitable for routine generation of germline modified animal models

This paper improves the GONAD method for in situ genome editing, achieving efficiency comparable to microinjection for generating germline modified animal models.

An Efficient CRISPR Protocol For Generating Conditional And Knock-In Mice Using Long Single-Stranded DNA Donors

This paper introduces an efficient CRISPR protocol using long single-stranded DNA donors to generate conditional and knock-in mice with higher success rates than existing methods.

Easi-CRISPR: Efficient germline modification with long ssDNA donors

Easi-CRISPR enables efficient germline modification with long ssDNA donors, achieving up to 100% efficiency in generating knock-in and floxed alleles in mice.