Kyle E. Orwig

Extrachromosomal Circular DNA Function as Mobile Elements to Alter the Mammalian Germline Genome

This study shows that extrachromosomal circular DNA (eccDNA) in the male germline can integrate into the genome and contribute to structural variations, with diabetes increasing eccDNA levels in sperm.

Epithelial stem cells in the premenopausal human vagina

The paper identifies putative vaginal epithelial stem cells (veSCs) in the premenopausal human vagina using single-cell RNA sequencing and functional assays.

Tex11 Mutant Mouse Models of Human Azoospermia

The paper validates three TEX11 variants associated with human azoospermia by generating mouse models and observing fertility and spermatogenesis defects.

A hidden protamine PTM code in sperm generates heterogeneous chromatin states and finetunes reproductive fitness

This study shows that protamine PTMs create heterogeneous chromatin states in sperm, affecting fertility and chromatin integrity.

Functional and Clinical Implications of Extrachromosomal Circular DNA in the Human Germline

This study identifies eccDNA in human male germline and links its abundance to chronic diseases through transcriptional activity and apoptosis signaling.

Single-Cell Analysis of Human Testis Aging, and Impact of Elevated Body Mass Index

This study uses single-cell RNA-seq to analyze age-related changes in human testis and finds that dysregulation correlates with BMI in older men.

In vivo versus in silico assessment of potentially pathogenic missense variants in human reproductive genes

The paper evaluates the functional impact of missense variants in human reproductive genes using mouse models and finds that many predicted pathogenic variants do not cause detectable phenotypes.

Tcf21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration

This study identifies Tcf21+ mesenchymal progenitors in mouse testis that contribute to somatic cell development, homeostasis, and regeneration.

Single-cell RNA sequencing of human, macaque, and mouse testes uncovers conserved and divergent features of mammalian spermatogenesis

The paper uses single-cell RNA sequencing to compare spermatogenesis in human, macaque, and mouse testes, identifying conserved and divergent molecular features across species.

Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche

This study validates the differentiation of rhesus macaque primordial germ cell-like cells (rPGCLCs) using transplantation into adult gonadal niches, showing they commit to late PGCs and spermatogonia-like cells but not ENO2-positive spermatogonia.