Christopher R. Sibley

The Structured RNA-binding Domains and Condensation Capacity of FUS Shape its RNA-binding Landscape and Function.

This study shows how FUS's RNA-binding and condensation properties shape its function in nuclear organization and DNA damage response through distinct and synergistic mechanisms.

Abundant capped RNAs are derived from mRNA cleavage at 3'UTR G-Quadruplexes

The paper identifies 3UTR-derived capped RNAs, showing they are abundant and originate from AGO2-mediated cleavage at G-quadruplexes, with implications for APP protein regulation.

Distinct neuroinflammatory signatures exist across genetic and sporadic ALS cohorts

The paper identifies two distinct neuroinflammatory signatures in ALS cohorts using gene expression analysis and validates their association with clinical metrics.

Random forest modelling of neuropathological features identifies microglial activation as an accurate pathological classifier of C9orf72-related amyotrophic lateral sclerosis

The paper uses random forest modelling to identify microglial activation as a key classifier for C9orf72-related ALS and shows spatial FUS staining changes predict disease status.

An improved iCLIP protocol

The paper introduces an improved CLIP protocol (iiCLIP) that produces more specific RNA binding site datasets compared to existing methods.

A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle

The paper introduces a microfluidic system for single-cell transcriptome profiling and demonstrates its ability to identify cell cycle regulators.

A systems view of spliceosomal assembly and branchpoints with iCLIP

The paper uses spliceosome iCLIP to map human spliceosome engagement with snRNAs and pre-mRNAs, identifying over 50,000 branchpoints and their associated splicing factor binding patterns.

Individual nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to determine protein-RNA interactions.

The paper describes the iCLIP method for high-throughput, nucleotide-resolution mapping of protein-RNA interactions.

Identification of Brain Expression Quantitative Trait Loci Associated with Schizophrenia and Affective Disorders in Normal Brain Tissue

This study identifies brain expression quantitative trait loci (eQTLs) associated with schizophrenia and affective disorders using genetic and transcriptomic data from human brain tissue.