Karen Tsay

A water-pinning hotspot drives templated tau aggregation

In a minimal tau fibril model, residue 300/301 binds incoming peptide first and carries the most release-prone hydration water. Spin-labelling site 300 impairs locking and suppresses seeded growth.

tRNA-derived fragments elevated in Alzheimer's disease facilitate Tau aggregation

This study identifies tRNA-derived fragments (tRFs) as key regulators of Tau aggregation in Alzheimer's disease and shows their potential as therapeutic targets.

Structure-specific Mini-Prion Model for Alzheimer's Disease Tau Fibrils

The paper designs synthetic tau fibrils that mimic AD and CTE structures, showing strong seeding ability and catalytic effects in vitro and in cells.

DEER of Singly Labelled Proteins to Evaluate Supramolecular Packing of Amyloid Fibrils

This study introduces DEER spectroscopy to analyze the supramolecular packing of amyloid fibrils, showing that heparin-induced fibrils are mispacked while seeded aggregation improves packing order.

Localized Reconstruction of Multimodal Distance Distribution from DEER Data of Biopolymers

This paper introduces SF-SVD and WavPDS methods to accurately reconstruct distance distributions from DEER data of biopolymers, resolving broad and mixed features without prior knowledge.

Passaging Human Tauopathy Patient Samples in Cells Generates Heterogeneous Fibrils with a Subpopulation Adopting Disease Folds

The paper shows that cell-passaged tau fibrils from CBD and PSP patients generate heterogeneous fibrils with a subpopulation resembling the original disease fold.