wet-lab / biochemical assay

This study shows EMC facilitates membrane protein biogenesis by regulating membrane fluidity through ergosterol homeostasis in fission yeast.

The paper develops a proteomic approach to study E. coli ABC importers, revealing abundance variations, inverse correlations between substrate-binding protein affinities and permease interactions, and counterintuitive stoichiometries.

This study shows that fascin is essential for axon development, brain wiring, and function in both cultured neurons and Drosophila melanogaster.

This study identifies N2G as an F-actin bundler and kinesin-1 activator that mediates active microtubule-actin crosstalk, crucial for nuclear positioning.

This study shows that the ketogenic diet reduces colorectal tumors in mice by altering the gut microbiome to increase stearic acid, which suppresses cancer cell growth and immune responses.

This study investigates how actin-binding domain interactions with actin and catenins contribute to the formation and maturation of adherens junctions through oligomerization and reorganization.

The paper identifies CNBP as a factor required for mRNA localization to cell protrusions by forming a motor-adaptor complex with KIF1C.

This study identifies new PPM1D inhibitors using a drug discovery platform and shows their distinct biological effects compared to other DDR and p53 pathway modulators.

The paper identifies a CGRP/Ramp1 axis-mediated interaction between nociceptive neurons and gastric cancer cells that promotes tumor progression.

The paper identifies PITPβ's role in COPI vesicle fission by delivering phosphatidylcholine through membrane contacts, revealing a mechanism for lipid transfer in intracellular transport.

This study identifies lung CD4+ resident memory T cells and their interaction with airway secretory cells as key regulators of neutrophilic allergic airways disease in mice and humans.

This study uses endoluminal biopsy and single-cell proteomics to analyze immune profiles in intracranial aneurysms, identifying immune signatures linked to aneurysm stability and rupture.

This study identifies a pro-myogenic domain in CYTOR and shows that chemically modified RNA delivery improves dystrophic myotube function.

This study shows neuroblastoma cells are hypersensitive to PLK4 inhibitor RP-1664 due to dual mechanisms of centriole loss and amplification, leading to cell death.

The paper shows MAGE-A4 promotes NSCLC by recruiting IgA+ plasma cells, reducing tumor burden and enhancing T cell activity.

This study identifies TRPV4 as the dominant mechanosensor for shear-induced traction responses in endothelial cells, surpassing Piezo1 in both initial traction rise and long-term relaxation.

The paper identifies two distinct mechanosensory neuron types in Nematostella vectensis, showing structural, molecular, and developmental diversity in cnidarian mechanoreceptors.

This study identifies AnfO as a protein that prevents misincorporation of incorrect cofactors into Fe-only nitrogenase during its maturation.

This study investigates VZV gene repression in neuronal cells, finding that histone deposition is absent at quiescent viral genomes.

This study shows that phosphate limitation in Gram-negative ESKAPE pathogens triggers alternative lipid synthesis, enabling antibiotic tolerance through reduced interaction with cationic antimicrobial peptides.

The paper shows that the MinC/MinD complex enhances DNA binding, with the MinC linker playing a critical role, and links this to chromosome organization in E. coli.

This study reveals how zyxin assemblies coordinate actin nucleation and crosslinking to repair stress fibers in response to mechanical forces.

This study identifies MAIT cells as protective against sterile lung injury by promoting anti-fibrotic pathways through cDC1 accumulation.

This study identifies Mdm38/LETM1 as a protein that links ion homeostasis and proteostasis in the mitochondrial inner membrane in yeast.

This study identifies CARD-like domains in bacterial defense systems that activate gasdermin-mediated cell death against phage infection.

The paper identifies a unique population of regulatory T cells in the murine meninges that maintain brain homeostasis by suppressing interferon-gamma-producing lymphocytes and preventing hippocampal damage.

The paper shows that tumor-derived glucocorticoids increase ROR1 expression while suppressing T cell activity, and GC-resistant CAR-T cells can overcome this by preserving ROR1 expression in tumor cells.

This study shows that endothelial CTR1 senses disturbed flow by increasing mitochondrial copper, leading to cuproptosis and atherosclerosis, and identifies a therapeutic target.

This study identifies unconventionally primed TFH1 cells as essential for generating IgA+ long-lived plasma cells during mucosal viral infections.

This study shows that continued milk feeding post-weaning enhances gut barrier integrity via microbiome-driven JNK2 activation, but only if initiated during the weaning period.