wet-lab / biochemical assay

This study shows influenza virus uses its nucleoprotein to block RIG-I activation by host RNAs, thereby suppressing the antiviral response and enhancing viral replication.

The paper introduces synthetic tools to redirect and chemically control the Arg/N-degron pathway by modifying PRT1's substrate recognition and using nanobodies to induce ubiquitination.

The paper shows that glutaminolysis and mitochondrial metabolism are conditionally crucial for B cell differentiation and cytokine signaling, revealing metabolic regulation of plasma cell generation and cytokine receptor signaling.

The paper identifies an RNA-binding protein essential for female gamete development and sporozoite infectivity in Cryptosporidium by analyzing single-cell data and conducting conditional ablation experiments.

This study investigates the oligomerization and activation mechanism of the RPS4-RRS1 immune receptor complex in Arabidopsis, showing that effector recognition triggers conformational changes that enable NADase activity.

This study identifies IKKβ activation as a transient upstream signal triggered by efferocytosis that promotes tissue resolution through multiple downstream pathways.

This study identifies cyanoquinoline-based HIPK4 inhibitors with nanomolar potency and reveals their cell-type-specific effects on HIPK4 proteostasis and autophagy.

This study identifies and characterizes monoclonal antibodies from rabbit B cells that bind intestinal bacteria, demonstrating their broad specificity and potential for therapeutic applications.

This study develops human spinal cord organoids and assembloids to model oligodendrocyte development and disease states, showing they recapitulate developmental signatures and cytokine-induced dysfunction seen in multiple sclerosis.

This paper introduces an in vivo TCE platform that depletes plasma cells in autoimmune dry eye models, showing efficacy and safety without ex vivo manufacturing.

This study shows that chemotherapy, specifically CarboTaxol, synergizes with cancer vaccines and anti-PD-1 to improve tumor control and survival by expanding stem-like TCF1+CD8+ T cells.

This study identifies a genetic interaction between MCL1 and MARCHF5 in PEL cells, showing that MARCHF5 promotes cell survival by degrading NOXA, an antagonist of MCL1.

This paper identifies cereblon (CRBN) as a host factor that promotes influenza virus replication by degrading AMPK, shifting cellular metabolism to support viral growth.

The paper shows that MCA1 mechanosensitive channels mediate rapid inter-root signaling in Arabidopsis following mechanical injury.

This study identifies a novel peptidoglycan recycling pathway in mycobacteria that bypasses typical intermediates, revealing metabolic flexibility in cell wall recycling.

This paper modifies Nab-paclitaxel with receptor binders to improve tumor targeting and reduce toxicity, showing enhanced efficacy in cancer cells and xenograft models.

This study uses reverse genetics in humanized mice to show that DPP9 deficiency causes CARD8-mediated pyroptosis in human hematopoietic stem and progenitor cells, leading to pancytopenia.

This study identifies SLAP as a tumor suppressor that limits SFK activity in colonic epithelial cells, preventing tumorigenesis and hyperproliferation.

The paper investigates paralog-specific effects of DGK inhibitors on PA generation during PLC signaling in live cells, finding that BMS-502 inhibits PA production while R59022 paradoxically increases it.

This study shows midbody inheritance in HSCs delays re-entry into the next cell cycle but does not affect lineage potential.

This study shows neutrophils maintain high migration speed in capillaries of varying sizes by adapting their actomyosin cytoskeleton via the ROCK-MyoII pathway, preventing vascular obstruction.

This study identifies PRMT6-mediated arginine methylation of NADK that suppresses its kinase activity and NADP+ synthesis, revealing a regulatory mechanism involving coordination with Akt phosphorylation and implications for cancer progression.

The paper investigates the regulation of cortical dynein and its interaction with microtubules during mitosis in C. elegans by analyzing the effects of depleting complex components and regulators.

This study develops a cryogel vaccine platform that generates oxygen to overcome tumor antigen tolerance and induce durable anti-tumor immunity in prostate cancer.

The paper shows that inhibiting apoptosis of alveolar myofibroblasts (AMFs) reprograms them into ductal myofibroblasts (DMFs) in a mouse model, suggesting fate plasticity in lung mesenchymal cells.

The paper investigates the role of histone methyltransferases in DNA elimination during sea lamprey embryogenesis, finding they contribute to repressive histone mark deposition but are not essential for the elimination process itself.

This study identifies that dCas9-KRAB mislocalizes to the cytoplasm in iPSC-derived neurons, leading to KRAB-specific degradation, and shows that adding a neuronal-specific NLS restores nuclear localization and expression.

This study identifies MtvS1 and MtvS2 as regulators of the Francisella type V-A CRISPR-Cas system and shows they interact with RNA polymerase to modulate gene expression.