fluorescence / live-cell microscopy

The paper develops biosensors to track conformational changes of GTPases in live cells, revealing dynamics of Cdc42 activity and microclusters involved in cell motility.

The paper shows that FACT facilitates Ubp10 release from nucleosomes after H2B deubiquitination, revealing release as the rate-limiting step for Ubp10 turnover.

The paper introduces SMAUG, a semi-automated tool for analyzing actin filament dynamics from TIRF microscopy images, reducing analysis time while maintaining accuracy.

SNAzzy processes and analyzes high-throughput microscopy data from Drosophila embryos to study synchronous network activity.

This study shows that evolution can restore cellular function without conserving microscopic organization, leading to novel molecular phenotypes in budding yeast.

The paper compares three F-actin probes in C. elegans, finding mStayGold offers best brightness and photostability, while mNeonGreen is suitable for photobleaching applications.

The paper develops a red fluorescent sensor, chILI, for detecting cAMP in living cells through intensity and lifetime imaging, demonstrating its use in mouse brain neurons.

This study measures how antibody-antigen interactions affect the fluorescence of Rhodamine 6G-conjugated antibodies in human serum, showing non-linear spectral changes.

This study assesses whether hydroquinone induces aneuploidy in hematopoietic stem cells using KaryoTap and FISH, finding no reproducible increase in whole-chromosome aneuploidy.

This study investigates how different molecular crowders affect the magnetic field-dependent fluorescence and recovery kinetics of MagLOV2 in E. coli lysate.

The paper presents a standardized experimental protocol and analysis framework for fluorescence-based CRISPR enzyme assays to improve reproducibility and consistency in kinetic measurements.

The paper introduces a Gateway-based cloning platform for efficient construction of rAAV vectors optimized for neuroscience applications, enabling precise optical interrogation of neural circuits with tools like ULoVE.

This study investigates how carbonosomes in Pseudomonas aeruginosa affect cell viability and antibiotic sensitivity during nutrient fluctuations and tobramycin exposure.

The paper investigates how β-tubulin isotypes TBB-1 and TBB-2 contribute to acentrosomal spindle bipolarity and function in C. elegans oocytes.

The paper shows that fluorescent proteins misreport ribosome abundance under translation inhibition, as fluorescence intensity does not track ribosome abundance during chloramphenicol treatment.

The paper uses microscopy and field theory to show transcriptional condensates exhibit size control through non-equilibrium dynamics.

The paper develops a FRET-force biosensor to measure mechanical tension on the nuclear pore complex and finds that nuclear strain, not cytoskeletal forces, primarily drives these forces.

The paper introduces non-covalent reversibly photoconvertible fluorescent proteins that change color under illumination, enabling discrimination of spectrally similar variants in live cells.

This paper provides a framework for measuring protein oligomerization equilibria using fluorescence correlation spectroscopy, demonstrating its application with PCNA under varying ionic strength conditions.

msaGUI is a no-code tool for analyzing and visualizing multispectral images through customizable workflows and exportable outputs.

The paper shows that DnaK, a bacterial chaperone, dynamically reallocates in response to proteostasis demand, with acute stress increasing client engagement frequency and lifetime.

The paper uses single-molecule techniques to reveal two distinct myosin cycles with different kinetics and stroke sizes affected by mavacamten and omecamtiv mecarbil.

QuantiTrack, a MATLAB GUI, unifies single-molecule detection, tracking, quality control and mobility analyses; for glucocorticoid receptor, hormone washout cut bound fraction, dwell times and low-mobility occupancy.

The paper introduces a split-GFP graphene grid platform for selective protein capture in cryo-EM, demonstrating improved enrichment and a 2.58 Å cryoEM reconstruction of GFP11-tagged apoferritin.

The paper introduces dual-mode Bio-CM2, a miniature mesoscope that captures fluorescence and label-free imaging simultaneously, demonstrating its use across different organisms and biological contexts.

The paper introduces a method for live-cell super-resolution microscopy using fluorogenic HaloTag ligands and BF dyes, enabling single-molecule localization and diffusivity mapping of proteins.

This paper introduces two palladium porphyrin compounds for ratiometric O2 quantification in microplates, enabling high-throughput biochemical assays by measuring fluorescence quenching.

The paper identifies the stimulus activating the fgMAPK pathway in yeast filamentous growth and links TOR pathway regulation of Gln3 to cell elongation.

The paper shows that intra-cluster receptor density (IRD) regulates TNFR1 signaling, with higher IRD activating and lower IRD impairing signaling.

The paper investigates the regulation of cortical dynein and its interaction with microtubules during mitosis in C. elegans by analyzing the effects of depleting complex components and regulators.