Biochemical Analysis and Sensing Techniques

Glucose appetition in C57BL/6J mice: Influence of nonnutritive sweetener experience, food deprivation state and sex differences

The paper investigates how nonnutritive sweetener experience, food deprivation, and sex affect glucose appetition in mice, finding that food restriction enhances glucose licking but is not needed for flavor preference.

Cyclophosphamide induces the loss of taste bud innervation in mice.

This study shows that cyclophosphamide reduces innervation of taste buds in mice, affecting both taste receptor cells and gustatory nerve fibers.

Novel Gurmarin-like Peptides from Gymnema sylvestre and their Interactions with the Sweet Taste Receptor T1R2/T1R3

The paper identifies eight gurmarin-like peptides from Gymnema sylvestre and shows that Gur-1 and Gur-2 interact with the T1R2/T1R3 sweet taste receptor, suggesting potential for antidiabetic drug development.

Ultrastructural localization of calcium homeostasis modulator 1 in the taste buds of mouse circumvallate papillae

The paper generates a monoclonal antibody against CALHM1 and localizes it to presynaptic membranes of channel synapses in mouse taste buds using immunoelectron microscopy.

Learning and memory deficits produced by aspartame are heritable via the paternal lineage

This study shows that aspartame consumption in mice causes heritable learning and memory deficits in offspring via the paternal lineage.

A Sweeter Future: Using protein language models for exploring sweeter brazzein homologs

This study uses protein language models to design sweeter and more thermostable brazzein variants, identifying mutations that improve heat resistance and sweetness.

An intra-oral flavor discrimination task in freely moving mice

This paper introduces a novel behavioral task for flavor discrimination in mice, showing that intraoral flavor, not olfactory input, is critical for task performance.

Inhibiting a promiscuous GPCR: iterative discovery of bitter taste receptor ligands

The paper discovers new ligands for the promiscuous TAS2R14 bitter taste receptor using a mixed experimental and computational approach, identifying 10 antagonists and 200 agonists.

T1R3 subunit of the sweet taste receptor is activated by D2O in transmembrane domain-dependent manner

The paper shows D2O activates T1R3 subunits in a transmembrane domain-dependent manner, with mutations impairing this activation.

Inflammation induces bitter taste oversensitization via epigenetic changes in Tas2r gene clusters

The paper shows inflammation increases Tas2r gene accessibility via chromatin remodeling, leading to heightened bitter taste responses in mice.

Intraoral thermal processing in the gustatory cortex of awake mice

This study investigates how thermal information from the mouth is encoded in the gustatory cortex of awake mice, showing that neurons encode thermal data independently of taste and that some neurons process both taste and temperature.

Experience-dependent plasticity of gustatory insular cortex circuits and taste preferences

The paper shows that early taste experience in mice affects adult sucrose preference through changes in insular cortex circuitry and inhibitory plasticity.

Salivary Glands treated with AAV vectors encoding PYY and exendin-4 impact upon taste perception and body mass accumulation in mice.

This study investigates the impact of AAV-mediated overexpression of PYY and exendin-4 in salivary glands on taste perception and body mass in mice, finding reduced body mass accumulation and altered taste responsiveness.

Rapid structural remodeling of peripheral taste fibers is independent of taste cell turnover

The paper shows that taste ganglion neuron arbors rapidly remodel their structure independently of taste cell turnover, maintaining a stable number of arbors over time.

The impact of familiarity on cortical taste coding

This study examines how familiarity with taste stimuli affects population-level taste coding in mouse gustatory cortex, finding reduced active cells and increased response magnitude with experience.

Changes of the biophysical properties of voltage-gated Na+ currents during maturation of the sodium-taste cells in rat fungiform papillae

This study examines changes in voltage-gated Na+ currents during the maturation of sodium-taste cells in rat fungiform papillae, finding shifts in biophysical properties and the emergence of persistent currents.

Active licking shapes cortical taste coding.

This study shows that taste discrimination in mouse gustatory cortex neurons is enhanced when spike rate and timing are analyzed relative to lick timing, indicating the lick cycle is critical for taste processing.

A T1R-independent mechanism for responses to hyperosmotic sugars involves a carbonic anhydrase-sensitive mechanism in Type III receptor cells

The paper identifies a T1R-independent mechanism for hyperosmotic sugar responses in mice, mediated by carbonic anhydrase in Type III taste bud cells.

Characteristics and Impact of the rNST GABA Network on Neural and Behavioral Taste Responses

This study investigates the role of GABAergic neurons in the rNST by optogenetically activating them and observing their impact on taste responses in non-GABAergic neurons.

Possible Functional Proximity of Various Organisms Based on Taste Receptors Genomics

The paper compares sweet and bitter taste receptor genomics across 20 vertebrates to assess functional proximity and ligand binding affinity similarities.

Sweet taste receptor cells may participate in mucosal immune surveillance

The paper identifies that taste receptor cells in mice share characteristics with M cells, suggesting they participate in mucosal immune surveillance and modulate taste responses to microbial signals.

A gut sensor for sugar preference

The paper shows that neuropod cells in the gut detect caloric sugars via SGLT1 or sweet taste receptors, guiding sugar preference by signaling the brain through glutamate release.

LiCl-induced sickness modulates spontaneous activity and response dynamics in rat gustatory cortex.

This study shows that illness alters sensory cortical responses to tastes in rats, reducing response magnitudes and changing coding strategies to reflect a 'good-bad' perception of palatability.

The presynaptic active zone protein Bassoon as a marker for synapses between Type lll cells and afferent nerve fibers in taste buds

The paper identifies Bassoon as a marker for synapses between Type III cells and afferent nerve fibers in taste buds using immunocytochemistry and immunoelectron microscopy.

In-silico investigation of umami peptides with receptor T1R1/T1R3 for the discovering potential targets: A combined modeling approach

The paper identifies key residues in umami peptides and their binding to T1R1/T1R3 receptors using computational methods and proposes a decision rule for predicting umami peptides.

Taste Bud Connectome: Implications for Taste Information Processing

The paper examines neural connectivity in mouse taste buds, finding that most nerve fibers connect to a single cell type, but some branch to connect to multiple types, challenging the labeled-line hypothesis.

Lasting effects of low-calorie sweeteners on glucose regulation, sugar intake, and memory

This study shows that early-life low-calorie sweetener consumption in rodents leads to long-term metabolic, behavioral, and neural impairments without weight gain.

Overlapping Representation of Basic Tastes in the Human Gustatory Cortex

This study uses fMRI to investigate taste representation in the human gustatory cortex and finds overlapping activation patterns for basic tastes.

Functional Molecular Switches of Mammalian G Protein-Coupled Bitter-Taste Receptors

The paper improves comparative modeling of mammalian bitter-taste receptors (TAS2Rs) by re-aligning sequences and constructing homology models, validated through site-directed mutagenesis and functional assays.

Fructose appetition in "taste-blind" P2X2/P2X3 double knockout mice

The paper shows that P2X2/P2X3 double-knockout mice develop strong fructose appetition, unlike TRPM5 KO mice, suggesting distinct mechanisms for fructose taste perception.