Photoreceptor and optogenetics research

The paper identifies 13 novel algal LOV domain proteins with potential applications in opto-biotechnology for controlling translational, ribogenetic, and proteolytic processes.

The paper identifies a KCR1 variant, C29D, that maintains stable potassium selectivity and provides reliable optogenetic inhibition across species and conditions.

The paper identifies a new family of rhodopsin channels in apusomonads that absorb ultraviolet to blue light and function as anion-selective channels involved in photoavoidance.

This study shows that microbial rhodopsins can transport organic anions, expanding their known substrate range and suggesting new optogenetic applications.

The paper identifies blue-shifted channelrhodopsins from ancyromonads that enable multiplex optogenetics by conducting chloride or cation currents with improved spectral properties.

The paper shows carotenoids enhance rhodopsin light utilization by accelerating photocycles and light-harvesting in marine bacteria.

This study identifies Lys91 as the key residue determining UV sensitivity in vertebrate Opn5, showing its role in spectral tuning and retinal isomer binding.

The paper identifies leaky Chrimson expression as a source of false-positive signals in optogenetic functional connectivity experiments and proposes a control to mitigate this issue.

This study develops a data-efficient and interpretable model of optogenetic inhibition using potassium and chloride opsins to analyze how parameters and physiological conditions affect seizure suppression in CA1 neurons.

This paper introduces ChReef, an improved optogenetic tool with enhanced performance in sensory and cardiac systems, demonstrating its effectiveness in restoring visual and auditory function.

The paper presents cryo-EM structures of FArhodopsin and proteorhodopsin, showing no retinal binding despite conserved lysine and similar fold to retinal-binding rhodopsins, with mutations identified that prevent retinal binding.

This paper develops NIR imaging and optogenetic tools using Blvra-/- knockout to enhance light-controlled transcription and in vivo glucose regulation.

The paper identifies traits of bathy phytochromes and develops a far-red light-activated optogenetic system called pFREDusk.

The paper develops an all-optical system to inhibit cortical neurons and monitor responses, showing widespread suppression of sensory responses after optogenetic silencing.

The paper characterizes CryoRhodopsins from cold environments, revealing their dual functionality and structural adaptations to low temperatures.

The paper reports the cryo-EM structure of a blue-shifted channelrhodopsin from Klebsormidium nitens and develops mutants with blue-shifted action spectra.

The paper reports light energy transfer from hydroxylated carotenoids to heimdallarchaeial rhodopsins in marine archaea, showing light-harvesting capabilities in archaea similar to bacterial rhodopsins.

This paper introduces a dual-color optogenetic system that enables high-frequency neuronal activation with red light and precise suppression with blue light.

The paper introduces R-LARIAT, a red light-induced protein clustering system that inhibits protein functions in HeLa cells by trapping GFP-tagged proteins, demonstrating high efficiency and stability.

This study demonstrates optogenetic modulation of peripheral sensory nerve activity in rats, showing induction and suppression of large-diameter afferents using ChR2 and eNpHR3.0.

This paper develops optogenetic tools to induce membrane rupture of specific organelles using engineered BAX proteins with light-sensitive domains.

This paper characterizes a blue-sensitive, Gi/o-biased, and bistable ciliary opsin from a fan worm and explores its spectral and signaling properties through mutagenesis.

The paper demonstrates that echinenone-functionalized OCP requires two photons for photoprotection, revealing a sequential 2-photon absorption mechanism unique to a single biological photoreceptor.

This study develops a K+-selective channelrhodopsin for transcranial optogenetic inhibition of neuronal activity, showing reduced seizure activity in mouse models.

The paper investigates the photophysics of FRET-opsin voltage indicators and demonstrates two-photon voltage imaging in live mice using optimized protocols.

This paper describes the engineering of soluble bacteriorhodopsin analogues and reports their crystal structure, demonstrating the potential of protein engineering for developing molecular tools.

This study shows that the CRY2/CIB1 system can be used to precisely control protein localization in E. coli, enabling manipulation of cellular functions like cytokinesis.

This study uses time-resolved crystallography and MD simulations to reveal structural changes in C1C2 channelrhodopsin during activation, showing how conformational shifts enable ion conduction.

The paper introduces LOOMINA, an optogenetic system that deactivates transcriptional activation in response to blue light, offering high adaptability and compatibility with various effector proteins.

This paper develops a red/far-red light-inducible photoswitch system for optogenetic control of gene expression in mice, demonstrating its application in treating metabolic diseases.