Discover, rate, and discuss biology preprints.

The paper develops a high-throughput system for measuring oxidative stress indicators using a 384-well plate format, reducing time and resource consumption while maintaining accuracy.

The paper presents the cryo-EM structure of HsNHE6, revealing its dimeric architecture, ion-binding site, and lipid-stabilized gate, along with insights into its disordered C-terminus and functional roles in ion exchange and disease.

This study identifies riboflavin catabolites that bind MR1, reduce its cell surface expression, and inhibit MAIT cell activation.

This study investigates psoriasin as a natural food preservative, showing its antimicrobial activity and potential for cost-effective production.

This study investigates the structure, folding kinetics, and dynamics of long poly(UG) RNAs, revealing a dynamic model for segmental register exchange and double pUG fold formation.

The paper develops an ATP-driven platform for C-terminal protein modification using a bacterial E1-like enzyme, enabling precise bioconjugation with diverse functional groups.

This study investigates the molecular response of Drosera capensis to jasmonic acid and feeding stimuli using RNASeq and metabolomics, revealing enzyme regulation and prey-dependent small molecule production.

This study shows CD163 forms multimeric complexes to recognize diverse haemoglobin-haptoglobin ligands, enabling detoxification.

The paper characterizes two GT61 enzymes in Sorghum bicolor, identifies their functions in xylan modification, and identifies a residue critical for sugar donor specificity through structural and biochemical analysis.

This paper determines the cryo-EM structure of human PGT and identifies disulfide bonds critical for its function and post-translational modifications.

This paper introduces a method using macrocyclic phage display to identify selective protease substrates, demonstrating its effectiveness with FAP and DPP4.

The paper uses anti-sense oligonucleotides to disrupt RNA interactions in bacterial ribosome assembly, revealing intermediate structures and domain consolidation during RNP formation.

The paper determines the cryo-EM structure of Ycf1 bound to oxidized glutathione and identifies a 'flex-pocket' mechanism for substrate binding and specificity.

This study identifies PRMT1 oligomerization as critical for its function in pancreatic ductal adenocarcinoma by revealing its structural basis and functional impact on RNA-binding proteins.

This paper shows that Cdc13 dimerization is required for dynamic DNA exchange on telomeres, using biochemical assays and mutants.

This study identifies functionally important structural modules of SChLAP1 that drive cancer cell proliferation and invasion in prostate cancer.

This study investigates the mechanism of C3 side chain fragmentation in cephalosporins by SME-1 class A carbapenemase using crystallography and QM/MM simulations, revealing that sp3 hybridization at C3 facilitates fragmentation while sp2 and sulfur at C3 inhibit it.

This study determines the cryo-EM structures of Aβ40 and medin fibrils in vitro, revealing polymorphic remodeling and mixed amyloid interactions.

This study shows that carbon monoxide exposure promotes growth and activity of carboxydotrophic bacteria in diverse soil ecosystems, suggesting a potential role for soils as a CO sink.

This paper characterizes a PNGaseA enzyme from Bifidobacterium bifidum that preferentially strips N-glycans from peptides over proteins, revealing its structural basis for substrate specificity.