Discover, rate, and verify biology preprints.

Optimizing 3D Spheroid Formation in Microwells via a Simple Pluronic F127 Coating

This paper evaluates the use of Pluronic F127 coating to enhance spheroid formation in microwells by reducing cell-substrate adhesion.

Comparative proteomic analysis of the composition of decellularized extracellular matrix (dECM) and dECM-based inks as compared to the native tissue

The paper compares the proteomic composition of decellularized extracellular matrix (dECM) and dECM-based inks to native tissues, finding increased collagen proportions in inks and a core matrisome shared across all dECMs.

Development of a rocking bioreactor strategy to augment pro-angiogenic factor secretion by human adipose-derived stromal cells

This study develops a rocking bioreactor system to enhance pro-angiogenic factor secretion by hASCs through dynamic culture and DAT coatings.

Re-examining the lower speed boundary of preferred coordination ratio constancy: estimator dependence and competing breakpoint regions

This paper re-examines the lower speed boundary of preferred coordination ratio constancy using published data, finding competing breakpoint regions rather than a single dominant boundary.

Systemic Nanobubbles Enable Ultrasound-Guided STING Immunotherapy in Breast Cancer

This paper develops SONATA, a nanobubble-based ultrasound-guided immunotherapy that activates the STING pathway in breast cancer, showing tumor growth inhibition and improved survival in mice.

Nanostructured Zirconia thin films as neurogliomorphic interface for neural cells of central and peripheral nervous system

This study examines how nanostructured zirconia thin films affect glial calcium signaling in astrocytes and neuron-glia co-cultures, showing enhanced glial responses compared to flat substrates.

Open-source benchmarking of dairy and dairy-free products

This study benchmarks sensory properties of dairy and dairy-free products across ten categories, finding significant gaps in mozzarella, Greek-style yogurt, and butter.

Intrinsic antifungal activity of curli nanofibers expands the design space for programmable antimicrobial biomaterials

The paper discovers that wild-type curli nanofibers have intrinsic antifungal activity and shows that fusing them with heliomicin enhances this activity against Candida albicans.

2-arm-PEG-oligocations transiently shield the liver sinusoids to mitigate off-target hepatic expression of mRNA lipid nanoparticles

This paper demonstrates that 2-arm-PEG-oligocations transiently shield liver sinusoids to reduce off-target hepatic mRNA expression and improve iLNP delivery to spleen and tumors.

Aged Tendons Have Impaired Mechanosensitivity and Lower Thresholds for Injury under Dynamic Compression

An Implantable Poly(pro-curcumin) Film for Local, Long-Lasting Neuroprotection After Spinal Cord Injury

This paper introduces an implantable poly(pro-curcumin) film that provides long-lasting neuroprotection after spinal cord injury by locally delivering curcumin, improving locomotor recovery and reducing inflammation.

Assessing the fractional contributions of static, slow and fast dynamic scatterer components to the flow index derived by continuous wave diffuse correlation spectroscopy

This study evaluates how static, slow, and fast dynamic scatterer components affect the flow index measured by cw-DCS, showing significant underestimation when static and slow components are present.

β-lactoglobulin a new whey: Computational redesign improves stability and nutritional composition

The paper uses computational methods to redesign β-lactoglobulin, improving its stability and BCAA content, with experimental validation of the results.

Focused framework sampling recovers binding-positive humanized anti-amyloid-β antibodies in a single sorting round

The paper develops a workflow combining immune yeast Fab display with focused humanization to identify binding-positive humanized anti-Aβ antibodies in a single sorting round.

Evolved microbial diversity enables combinatoric biosensing in complex environments

The paper develops a framework using evolved microbial diversity and machine learning to enable combinatoric biosensing of multiple analytes in complex environments.

A cross-modal generative model for incomplete and degradedprostate MRI with multicentre clinical validation

This paper develops MSCNet, a cross-modal generative model for reconstructing and restoring prostate MRI sequences, showing improved image quality and diagnostic performance in multicentre validation.

Directing the Chondro-Fibro Axis via Early Microenvironmental Interactions to Enable Precise and Volumetric Cartilage Repair

This study investigates how modulating the Rho-ROCK pathway in microfracture clots can reprogram cells to promote chondrogenic rather than fibrotic repair in cartilage defects.

LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs

This paper introduces LUCas, a light-controlled Cas13a system that suppresses background activity and enables precise control of RNA detection, improving assay sensitivity and enabling multiplexed viral co-infection detection.

Insect olfaction-inspired biohybrid sensor array for selective airborne pheromone detection and early monitoring of invasive Rhynchophorus ferrugineus

This paper develops a biohybrid sensor array inspired by insect olfaction to detect airborne pheromones and monitor early infestation of red palm weevils.

A Mechanical Theory for the Formation of Short Association Fibers in the Brain

This study shows mechanical forces during cortical folding guide the formation of short association fibers in the brain, explaining their U-shaped trajectories and prevalence between gyri.