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Rapid Electrochemical Biosensing of Listeria monocytogenes Using Rationally Designed Host-Pathogen Interface Peptides

This paper develops a biosensor using host-pathogen interaction peptides to rapidly detect Listeria monocytogenes with high selectivity and sensitivity.

Balancing performance and complexity of dual-wedge prism-based spectroscopic single-molecule localization microscopy

This study compares DWP-based sSMLM configurations to balance localization and spectral precision with experimental complexity.

Engineered cell-like vesicles instruct keratocyte differentiation for corneal biofabrication and regeneration

The paper uses growth factor-loaded vesicles to direct keratocyte differentiation in 3D corneal constructs without repeated soluble growth factor supplementation.

Programmable Allosteric DNAzyme Coupled with CRISPR/Cas12a System for Multiplexed and Sensitive Detection of Extracellular Vesicle Derived MicroRNAs

This paper develops a CRISPR/Cas12a-based system for sensitive and multiplexed detection of EV-derived microRNAs using DNAzyme and machine learning for cancer classification.

Mapping 3D cellular mechanical activity with matrix-embedded DNA force-history probes

This paper introduces DNA-based force-history probes to map 3D cellular mechanical activity over time, enabling visualization of stress patterns in breast cancer spheroids.

Characterizing the Assembly and Functional Properties of Gene-Length Mixed DNA Monolayers on Electrodes for Cell-Free Expression

This study investigates gene-length DNA monolayers on gold electrodes for cell-free protein expression, showing tunable surface density and partial functional retention under certain conditions.

Photoinitiator-free visible-light bioprinting: minimising radical-induced oxidative stress and DNA damage in gelatin hydrogels

This paper introduces a photoinitiator-free gelatin hydrogel system that enables visible-light-induced crosslinking, reducing oxidative stress and DNA damage in cell culture.

Sex-Specific Remodeling Phenotypes of the Tricuspid Valve Leaflets in an Ovine Model of Functional Tricuspid Regurgitation

This study investigates sex-specific tricuspid valve leaflet remodeling in an ovine model of functional tricuspid regurgitation, revealing distinct structural, mechanical, and transcriptional responses across sexes and castration status.

Supramolecular Site-Specific Antibody Drug Conjugates Outperform Cysteine-Conjugated Analogs in Pancreatic Peritoneal Carcinomatosis

This paper develops a supramolecular ADC conjugation method that improves drug loading and efficacy in pancreatic cancer compared to cysteine-conjugated analogs.

Evolution-inspired multi-objective Bayesian optimization for protein engineering

This paper introduces EvoMOBO, a multi-objective Bayesian optimization framework for protein engineering, showing improved performance in enzyme tasks compared to wild-type variants.

An image-based framework for in silico trials of ablation strategies in scar-related ventricular tachycardia

This paper presents an image-based framework to simulate and compare ablation strategies for scar-related ventricular tachycardia using patient-specific models.

Improved recombinant protein production and scale-up fermentation of Aspergillus oryzae hyphal-dispersion hydrophobin-deficient strain

This study develops a hyphal-dispersion mutant of Aspergillus oryzae with reduced hyphal adhesion and improved enzyme production and bioreactor performance.

Assessing the Clinical Utility of Finite Element Analysis Using Post-operative CT-Derived Models: A Material Comparison of Multi-level Spinal Fusion Constructs

This study evaluates the clinical utility of finite element analysis (FEA) in assessing stresses in spinal fusion constructs using post-operative CT data and compares different implant materials.

Unlocking Your Programmable and Creative RNA Sequence Designer with RDiffusion

RDiffusion is a generative transformer model for RNA sequence design that outperforms baselines in success rate and diversity, and was used to design therapeutic miRNA mimics for osteoarthritis.

A biofilm-derived peptide as an underwater adhesive

The paper identifies a biofilm-derived peptide from Vibrio cholerae that adheres to surfaces and functions as a wet adhesive and flocculant, with potential for industrial and biomedical applications.

Optimizing CRISPR/Cas9 genome editing in primary human hematopoietic cells to advance studies into HIV biology

This study develops a CRISPR/Cas9 platform for editing primary human HSPCs to investigate HIV host factors and their impact on viral infection in vitro and in vivo.

Expanded stoichiometric model of chondrocyte metabolism: response to cyclical shear and compressive loading

This study develops a stoichiometric model of chondrocyte metabolism to examine how cyclical shear and compressive loading affect matrix protein synthesis, revealing sex-dependent differences and nitrogen limitations.

Differential Tissue Coupled Powering for Battery-Free Injectable Electroceuticals

This paper introduces a wireless powering method for injectable neural implants, demonstrating effective energy delivery and chronic stability in vivo.

Three-dimensional vascular microenvironments uncover endothelial plasticity during TGF-β2-driven vascular remodeling

This study shows that TGF-β2 induces endothelial plasticity and EndoMT in 3D vascular networks, revealing distinct responses compared to 2D cultures.

Data-efficient distal engineering of fluorinase using zero-shot models

This study uses zero-shot models to predict activity-enhancing mutations in fluorinase, leading to improved catalytic efficiency and stability in engineered variants.