A. Kobayashi

Mapping adipocyte interactome networks by HaloTag enrichment mass-spectrometry

The paper introduces HaloMS, a high-throughput method for mapping protein interactions in adipocytes, identifying new transcription factor complexes and networks.

High-throughput structure determination of an intrinsically disordered protein using cell-free protein crystallization

This paper develops a cell-free protein crystallization method to determine the structure of an intrinsically disordered protein and identifies key residues stabilizing its helical conformation.

Glutamine synthetase mRNA releases sRNA from its 3'UTR to regulate carbon/nitrogen metabolic balance

The paper shows that glutamine synthetase mRNA produces an sRNA, GlnZ, which regulates carbon/nitrogen metabolism by repressing SucA expression.

Cell-free Protein Crystallization for Nanocrystal Structure Determination

The paper develops cell-free protein crystallization (CFPC) to produce high-quality protein nanocrystals for structure determination, achieving a 2.11 Å resolution structure for CipA.

Metalloproteinase-dependent and TMPRSS2-independnt cell surface entry pathway of SARS-CoV-2 requires the furin-cleavage site and the S2 domain of spike protein

This study identifies a TMPRSS2-independent metalloproteinase-dependent entry pathway for SARS-CoV-2 that requires the furin cleavage site and S2 domain of the spike protein, highlighting new therapeutic targets.

Manipulating Histone Acetylation Leads to Adverse Effects in Hemangiosarcoma Cells

This study investigates the effects of histone acetylation manipulation using HDACi and BETi on canine hemangiosarcoma cells, finding that SAHA and JQ1 induce apoptosis and anti-tumour immunity, while JQ1 inhibits proliferation.

Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages

This study identifies ISOS-1 as a syngeneic mouse model for canine hemangiosarcoma and shows that tumor-associated macrophages are M2-polarized and express PD-L1, contributing to immune evasion.

Prevalence of floating toe and its relationship with static postural stability in children: The Yamanashi adjunct study of the Japan Environment and Childrens Study (JECS-Y)

This study examines the prevalence of floating toe in children and its association with static postural stability, finding a high prevalence and a correlation between floating toe score and postural stability metrics.

KDM2B promotes cell viability by enhancing DNA damage response in canine hemangiosarcoma

This study shows KDM2B promotes canine hemangiosarcoma cell viability by enhancing DNA damage response and suggests GSK-J4 as a potential therapeutic alternative.

An empirical model to predict survival curve and relative biological effectiveness after helium and carbon ion irradiation based solely on the cell survival after photon irradiation

The paper develops an empirical model to predict ion radiosensitivity, RBE, and survival curves using photon irradiation data across a range of LET values.

PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade

This study identifies equine PD-1 and PD-L1 sequences, shows anti-bovine PD-L1 mAbs cross-react with equine PD-L1, and demonstrates PD-L1 blockade enhances Th1 cytokine production in equine immune cells.

FoxM1 drives proximal tubule proliferation during repair from acute kidney injury

This study identifies FoxM1 as a key driver of proximal tubule proliferation during repair from acute kidney injury, revealing an EGFR-FoxM1 signaling pathway.