B. Dupuy

A monoclonal antibody collection for C. difficile typing

The paper develops a monoclonal antibody collection targeting C. difficile strains to improve research on clinically relevant ribotypes.

Anti-S-layer monoclonal antibodies impact Clostridioides difficile physiology

This study identifies monoclonal antibodies targeting C. difficile's S-layer protein, revealing both protective and detrimental effects on bacterial physiology.

HRMAS 13C NMR and genome-scale metabolic modeling identify threonine as a preferred dual redox substrate for Clostridioides difficile

The TcdE holin drives toxin secretion and virulence in Clostridioides difficile.

This study shows that TcdE holin is essential for toxin secretion and virulence in C. difficile, as tcdE mutants exhibit reduced toxin release and less virulence in mice.

Central in vivo mechanisms by which C. difficile's proline reductase drives efficient metabolism, growth, and toxin production

This study shows that proline reductase in C. difficile is essential for metabolism, growth, and toxin production in vivo, as its absence delays colonization and disease progression in mice.

Extracellular succinate induces spatially organized biofilm formation in Clostridioides difficile

This study shows that extracellular succinate induces biofilm formation in C. difficile, leading to thicker, more complex biofilms associated with infection relapse.

The cell wall lipoprotein CD1687 acts as a DNA binding protein during deoxycholate-induced biofilm formation in Clostridioides difficile

This study identifies CD1687 as a DNA-binding lipoprotein required for DCA-induced biofilm formation in C. difficile by promoting cell-matrix interactions through eDNA binding.

Elucidating dynamic anaerobe metabolism with HRMAS 13C NMR and genome-scale metabolic modeling.

This study uses HRMAS 13C NMR and genome-scale metabolic modeling to analyze dynamic anaerobic metabolism in Clostridioides difficile, revealing metabolic integration of glycolytic and amino acid fermentation pathways.

c-di-AMP signaling is required for bile salts resistance and long-term colonization by Clostridioides difficile

This study identifies c-di-AMP as a key signaling molecule in Clostridioides difficile that regulates osmolyte uptake and bile salt resistance, essential for gut colonization.

Bacteroides thetaiotaomicron uses a widespread extracellular DNase to promote bile-dependent biofilm formation

This study identifies bile as a trigger for biofilm formation in Bacteroides thetaiotaomicron and shows that extracellular DNA degradation by DNase BT3563 is essential for this process.

Real-time HRMAS 13C NMR of obligately anaerobic cells identifies new metabolic targets in the pathogen Clostridioides difficile

This study uses real-time HRMAS 13C NMR to analyze metabolism in C. difficile, identifying new metabolic integration points and informing a genome-scale model.

Metabolic adaption to extracellular pyruvate triggers biofilm formation in Clostridioides difficile

This study identifies extracellular pyruvate as a trigger for biofilm formation in C. difficile in the presence of DOC, revealing a mechanism for pathogen persistence in the gut.

Identification of RNAs bound by Hfq reveals widespread RNA partners and a sporulation regulator in the human pathogen Clostridioides difficile

The paper identifies RNAs bound by Hfq in C. difficile, revealing new RNA partners and a role for one ncRNA in sporulation regulation.

Ser/Thr kinase-dependent phosphorylation of the peptidoglycan hydrolase CwlA controls its export and modulates cell division in Clostridioides difficile

This study identifies CwlA as a substrate of the STK PrkC in C. difficile, showing that phosphorylation regulates its export and modulates cell division by controlling peptidoglycan hydrolysis.

Type I toxin-antitoxin systems contribute to mobile genetic elements maintenance in Clostridioides difficile and can be used as a counter-selectable marker for chromosomal manipulation

The paper characterizes type I toxin-antitoxin systems in C. difficile prophages, showing their role in prophage stability and introducing them as counter-selectable markers for chromosomal manipulation.

The mechanisms of in vivo commensal control of Clostridioides difficile virulence

This study identifies how commensal bacteria influence Clostridioides difficile virulence through metabolic and regulatory mechanisms in a mouse model.

An RNA-binding protein secreted by Listeria monocytogenes activates RIG-I signaling

This study identifies Zea, an RNA-binding protein secreted by L. monocytogenes, which stabilizes bacterial RNAs and activates RIG-I signaling to enhance interferon β production and modulate virulence.

Structure and assembly of a Clostridioides difficile spore polar appendage

This study investigates the structure and role of the polar appendage in Clostridioides difficile spores, showing its impact on germination and virulence.